Topical anti-inflammatory and anti-oxidative effects of porcine placenta extracts on 2,4-dinitrochlorobenzene-induced contact dermatitis

Abstract

BackgroundThe placenta is a reservoir enriched with growth factors, hormones, cytokines and minerals. While several beneficial effects of placenta extracts on wound healing, anti-aging and anti-inflammatory responses have been reported, relatively limited mechanistic exploration has been conducted to date. Here, we provide compelling evidence of anti-inflammatory and anti-oxidative activities of porcine placenta extracts (PPE) against contact dermatitis in vivo.MethodsA contact dermatitis mouse model was established by sensitizing the dorsal skin of BALB/c mice using the contact allergen, 2,4-dinitrochlorobenzene (DNCB), and molecular consequences of topical application of PPE were investigated. PPEs were pre-sterilized via γ-irradiation, which is a milder but more effective way of sterilizing biomolecules relative to the conventional autoclaving method.ResultsDNCB-induced skin lesions displayed clear contact dermatitis-like symptoms and topical application of PPE dramatically alleviated both local and systemic inflammatory responses. Inflammatory epidermal thickening was completely abrogated and allergen-specific serum IgE levels significantly reduced in the presence of PPE. Moreover, anti-oxidative activities of PPE were observed both in vitro and in vivo, which may lead to attenuation of inflammatory responses. Prolonged treatment with PPE strongly inhibited production of DNCB-induced reactive oxygen species (ROS) and subsequently prevented oxidative degradation of hyaluronic acid (HA), which triggers innate inflammatory responses.ConclusionOur findings supply valuable insights into the mechanisms underlying the anti-inflammatory effects of PPE and provide a functional basis for the clinical application of PPE in inflammatory diseases.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2396-1) contains supplementary material, which is available to authorized users.

Improvement of Skin Hydration Percentage by Intervention of Sheep Placenta Cream in Elderly Population at STW Cibubur Period September 2019

“…Epidermal thickening and allergen specific serum IgE were significantly reduced. (Heo, et al, 2018) Porcine placenta shows to have anti aging effects, whitening, and anti-wrinkle effects which are useful for treating skin condition that occurs in elderly. (Young, et al, 2010) A randomized controlled trial to compare the effects of topical placenta extract cream and gel in treatment of chronic wound shows that placental extract gel and cream have similar scores in Microscopic Angiogenesis Grading System (MAGS).…”

“…The WHO census estimates the increase in the elderly population in the world by 10% to 22% or from 800 million people to 2 billion people. The elderly population in Asia and Southeast Asia are 7% and 6% of the total demographic (Population Reference Bureau, 2016a, 2016b, 2017, 2018. The Population Reference Bureau (PRB) estimates that Indonesia will get a population surge to 365.3 million by 2030 which will further affect the surge in the elderly population and increase health problems that occur in the elderly.…”

Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or placental tissue promoted HUVECs angiogenesis in vitro

“…Furthermore, placental tissue-derived CM, similar to placental extract, has also been used in many disease treatments in recent years, such as in ischemic diseases, in scaffolds for engineered tissue [52], to treat osteoarthritis [53], for regenerating sciatic nerves [54], and in burn injuries, chronic ulcers, skin defects [55,56], climacteric symptoms [57], and hair loss [58]. These uses are mainly associated with the biological properties of the placental tissue, such as proangiogenic effects, wound protection [55,56], anti-in ammatory and antioxidative effects, anti-platelet aggregation activity [59,60], anti-aging, and low immunogenicity. The most fundamental reason is that the placenta contains abundant extracellular matrix (ECM) components and bioactive molecules [52,61].…”

NLRP3 Inflammasome and Allergic Contact Dermatitis: A Connection to Demystify

“…In the context of ACD, the treatment of keratinocytes with phenylenediamine (PPD) and 2,4-dinitrochlorobenzene (DNCB), increased the synthesis of HA through the induction of Hyaluronan Synthases (HS) 1 and 2, resulting in the formation of proinflammatory LMWHA fragments that interacted with TLR4 and activated the NF-κB signaling axis [ 55 , 56 ]. DNCB-induced LMWHA formation can also occur via ROS production [ 57 ]. Accordingly, several in vivo studies show that blocking ROS production and HA degradation (by antioxidants or hyaluronidase-inhibitors) prevents the sensitization and elicitation phases, in mice models of CHS [ 54 ].…”