Total alkaloid content in various fractions of <i>Tabernaemonata sphaerocarpa</i> Bl. (Jembirit) leaves

Salamah, Nina; Ningsih, D S

doi:10.1088/1757-899x/259/1/012017

Cited by 12 publications

(5 citation statements)

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“…The power of such factors for extraction of metabolites from Jembirit leaves was also reported by Salamah and Ningsih 34 . The significant influence of these parameters for alkaloid extraction in plant Swietenia mahagoni was presented by Ayuni and Nyoman 35 .…”

Section: Resultssupporting

confidence: 68%

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2019

IJPSR

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Merr.) is a genetically diverse plant known for its immense medicinal properties due to the presence of bioactive compounds among which alkaloids are most vital. So, the present study aimed to estimate the total alkaloid content in wild and in-vitro regenerated plant & callus tissue of Tinospora cordifolia. Different solvents, i.e., methanol, ethyl acetate, water, diethyl ether, and chloroform, have been tried and compared for their extraction efficiency. The presences of alkaloids were tested via Wagner's and Mayer's reagents. The quantitative estimation had been made by UV/Vis spectrophotometer using a dye-Bromocresol green. The methanol extracts of wild plant's stem showed the highest concentration (3.3%) followed by water (1.85%) and ethyl acetate (0.99%). The alkaloid content in chloroform and diethyl ether extracts of wild plant's stem was 0.49% and 0.28% respectively. The in-vitro regenerated plant extracts also showed the presence of alkaloids (though in minute amount) in all solvents viz. methanol (0.95%), diethyl ether (0.060%), chloroform (0.012%), water (0.80%) and ethyl acetate (0.67%). The methanol, water and ethyl acetate extracts of callus tissue hold alkaloids in the order-0.79%, 0.65%, and 0.39% but the diethyl ether and chloroform extracts displayed the absence of alkaloids. So, methanol was found to be the most suitable solvent for extraction of alkaloids. This work further proves that in-vitro regenerated plant and also the callus tissue of Tinospora cordifolia retain their parental characteristics of alkaloid biosynthesis, which can be enhanced further by altering the physical and hormonal parameters of the cultural environment.

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Section: Resultssupporting

confidence: 68%

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2019

IJPSR

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“…At pH<7 most compounds containing tertiary amine groups or quaternary ammonium salts, form with BCG yellow complexes extractable by chloroform (Khoi, 1983). This spectrophotometric procedure is simple and sensitive, and BCG can react with the alkaloids having nitrogen inside their structure (Fazel et al, 2010;Ajanal et al, 2012;Liu and Liu, 2015;Salamah and Ningsih, 2017). After the protonation in an acidic medium, the protonated nitrogen (quaternary ammonium cations) is the target for the reaction with the BCG compound.…”

Section: Resultsmentioning

confidence: 99%

Spectrophotometric and Chromatographic Determination of Alkaloids and Nicotine Contents in Lebanese Tobacco Leaves

Elchamieh,

Jaber,

Ibrahim

et al. 2024

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Nicotiana Tabacum; the annual herb; known as Tobacco from the Solanaceae family was known for its alkaloids and especially nicotine (NCT) content; smoking products, insecticides, anthelminthic activity and clinically proven therapeutics are examples of its uses. Herein two different methods were applied in an attempt to quantify the total alkaloids and NCT content in Nicotiana tabacum cultivated in Lebanon. Total alkaloids were investigated through the formation of a complex with bromocresol green under a simple spectrophotometric method. Whilst HPLC-DAD was the choice for the quantitation of NCT levels. The column was Lichrospher select B (5 µm, 250x4 mm), the temperature was set at 29 ⁰C and the wavelength at 260 nm. The mobile phase consisted of 2 M O-phosphoric acid and methanol (60:40, v/v) using isocratic elution at 1 mL/min. A linear relationship was proved under both instruments. The extraction yield of alkaloid totum ranges between 2.1 ± 0.25 and 6.8% ± 0.58 and alkaloids contents range from 12.14 ± 2.01 to 53.12 ± 4.54 mg of AE/g of extract for Ghandouriyeh and Danniye samples respectively. On the other side among the different areas which cultivated Tobacco in Lebanon, Danniye was found to have the highest NCT concentration of dry weight (2.64%) while Al-Hissa possesses the lowest content (0.75%). Even if the results were generally similar to other countries, the study showed a difference in values from one region to another.

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“…TLC densitometry is a quantitative analysis method that is simple, accurate, precise, easy, suitable for routine quality control (Misra et al, 2008). The wavelength for the detection of carpaine isolate at the densitometer was evaluated from the scanning of 200-700 nm alkaloid compounds (Sushmitha et al, 2018). The scanning results showed that the maximum wavelength produced was 501 nm.…”

Section: Resultsmentioning

confidence: 99%

Screening of Extraction Process and The Estimation of Total Alkaloids in Carica papaya Linn. Leaf

et al. 2020

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The papaya leaf decoctions have been widely used as a traditional treatment for malaria. The alkaloid of papaya leaves, carpaine, is reported to have the antiplasmodial activity in vitro. The industrial-scale commercial product development requires the quantification of the carpaine, and total alkaloids in the papaya leave as the raw materials. This study aims to optimize the extraction process leading to the highest yield and total alkaloids. The extraction process was carried out using different methods and solvents. The first method was maceration with the water-miscible solvent of ethanol: distilled water: HCl 37% (89: 10: 1 v/v/v). The second method was maceration with the water-immiscible solvent of dichloromethane solvents in pH 8-9 with ammonium hydroxide. The third method was digestion with acid water solvent of 0.05 N HCl. The identification of alkaloids employed the analysis using Thin Layer Chromatography (TLC) and Dragendroff reagent. The quantification of carpaine used a densitometer, while the spectrophotometric method was used to estimate the total alkaloids. The results showed that despite the extraction methods used, the tested extracts confirm the alkaloids content with the highest yield of 3.09% (84.72% of total alkaloids).

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Total alkaloid content in various fractions of Tabernaemonata sphaerocarpa Bl. (Jembirit) leaves

Cited by 12 publications

References 1 publication

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Spectrophotometric and Chromatographic Determination of Alkaloids and Nicotine Contents in Lebanese Tobacco Leaves

Screening of Extraction Process and The Estimation of Total Alkaloids in Carica papaya Linn. Leaf

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