Tumbuhan sapu-sapu (Baeckea frutescens L.) merupakan salah satu jenis keanekaragaman hayati yang tumbuh dan persebarannya cukup banyak di Indonesia. B. frutescens L diketahui memiliki senyawa metabolit sekunder aktif yang dapat dimanfaatkan sebagai obat, antibakteri, dan antioksidan. Penelitian ini dilakukan untuk menganalisis kandungan senyawa metabolit sekunder yang terdapat pada B. frutescens L. Metode yang digunakan adalah ekstraksi dilanjutkan dengan pengujian fitokimia kualitatif yang terdiri dari tujuh pengujian yakni uji fenol, tanin, flavonoid, saponin, alkaloid, steroid dan terpenoid serta pengujian kuantitatif yakni pengujian total fenolik ekstrak B. frutescens L. Hasil penelitian menunjukkan bahwa didapatkan persentase bobot rendemen ekstrak daun B. frutescens L. sebesar ekstrak n-heksan 5,39%, ekstrak etil asetat 14,54% dan ekstrak etanol yakni 19,81%. Hasil pengujian fitokimia kualitatif menujukkan senyawa fitokimia yang terkandung di dalam tumbuhan B. frutescens L. yakni pada ekstrak n-heksan hanya terdapat senyawa steroid dan pada ekstrak etil asetat terdapat senyawa fenolik, tanin, flavonoid, dan alkaloid. Selain itu, pada ekstrak etanol terdapat senyawa fenolik, tanin, flavonoid, saponin, steroid, dan alkaloid. Pengujian total fenolik ekstrak daun tumbuhan B. frutescens L. untuk pelarut etil asetat yakni 0,24% dan pelarut etanol yakni sebesar 0,14% dihitung terhadap senyawa fenol asam galat. Hasil penelitian ini diharapkan dapat menjadi informasi untuk penelitian lebih lanjut mengenai pemanfaatan kandungan metabolit sekunder B. frutescens L.
This research aimed to identify giant calotrope leaf (Calotropis gigantea) originated from Alue Naga and Ulee Lheu coast using combination method of Fourier Transform Infrared (FTIR) spectroscopy and Principal Component Analysis (PCA). FTIR spectra analyzed with PCA to identify the effect of the treatments against the sample which are dry leaf maceration, fresh leaf maceration, and plain fresh leaf against similarity and difference pattern between giant colatrope leaves from different areas, namely Alue Naga coast and Ulee Lheu coast. The result showed that samples were grouped into 4 groups, namely Alue Naga and Ulee Lheu macerated dry leaf group, Alue Naga and Ulee Lheu macerated fresh leaf group, Alue Naga fresh leaf group, and Ulee Lheu fresh leaf group with a variance of 97%. It can be concluded that giant calotrope fresh leaf can be identified according to its location with FTIR-PCA method, but the macerated leaf cannot.
The purpose of this study is to identify laban leaf which originates from Ie Seu'um, Gunung Paro, and Lambaro, with a combination of Spectroscopy Fourier Transform Infrared (FTIR) and Principal Component Analysis (PCA) methods. FITR spectrum pattern from each sample was identified as multivariate using PCA and classified based on similarity or variety of the spectrum. The PCA analysis results of laban leaf samples are separated and forming both good groups as well as diversity percentage which is 96%. The extracted groups can be used to identify the difference between geothermal and nongeothermal location compared to the fresh sample
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