Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats

Jing, Xiaoping; Cheng, Weiwei; Guo, Sheng; Ya, Zou; Zhang, Ting; He, Li

doi:10.1016/j.biopha.2017.09.038

Cited by 35 publications

(23 citation statements)

References 29 publications

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“…Since the use of TGs showed that it has toxic effects to kidneys and the reproductive system, TGs were thus introduced for experimental spermatogenesis disorder modeling (Du et al, 2018). Experimental studies also find that TGs treatment in male and female rats can cause pathological damages to the testis, epididymis, ovary, and uterus, and TGs can induce sperm abnormality and hormone expression abnormalities (Jing et al, 2017;Guo et al, 2019). In this study, TGs administration in rats for 4 weeks showed that testis tissues exhibited histopathological damage and cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Wuzi Yanzong Pill—Based on Network Pharmacology and In Vivo Evidence—Protects Against Spermatogenesis Disorder via the Regulation of the Apoptosis Pathway

Chen

Ding

et al. 2020

Front. Pharmacol.

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The crisis of male infertility is an issue of human reproductive health worldwide. The Wuzi Yanzong pill (WZYZP) is a traditional Chinese medicine prescription that shows efficacy in kidney reinforcement and essence benefit to ameliorate male reproductive dysfunctions. However, the pharmacological mechanisms of the WZYZP on male infertility have not been investigated and clarified clearly. This study was designed to investigate the effects of the WZYZP on spermatogenesis disorder and explore its underlying pharmacological mechanisms. First, based on a network pharmacology study, 39 bioactive compounds and 40 targets of the WZYZP associated with spermatogenesis disorder were obtained, forming a tight compound-target network. Molecular docking tests showed tight docking of these compounds with predicted targeted proteins. The protein–protein interaction (PPI) network identified TP53, TNF, AKT1, Bcl-XL, Bcl-2, and IκBA as hub targets. The Kyoto Encyclopedia of Genes and Genomes pathway network and pathway-target-compound network revealed that the apoptosis pathway was enriched by multiple signaling pathways and multiple targets, including the hub targets. Subsequently, the chemical characterization of WZYZP was analyzed using liquid chromatography to quadrupole/time-of-flight mass spectrometry, and 40 compounds in positive ion mode and 41 compounds in negative ion mode in the WZYZP were identified. Furthermore, based on the prediction of a network pharmacology study, a rat model of spermatogenesis disorder was established to evaluate the curative role and underlying mechanisms of the WZYZP. The results showed that WZYZP treatment improved rat sperm quality and attenuated serum hormone levels, reversed histopathological damage of the testis, reduced cell apoptosis in testis tissues, and ameliorated the expression of the predicted hub targets (TP53, TNF-α, AKT1, NFκB, and IκBA) and the apoptosis related proteins (Bcl-XL, Bcl-2, Bax, Caspase 3, and Caspase 9). These results indicated that the WZYZP has a protective effect on spermatogenesis disorder, suggesting that it could be an alternative choice for male infertility therapy.

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Section: Discussionmentioning

confidence: 99%

Wuzi Yanzong Pill—Based on Network Pharmacology and In Vivo Evidence—Protects Against Spermatogenesis Disorder via the Regulation of the Apoptosis Pathway

Chen

Ding

et al. 2020

Front. Pharmacol.

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“…However, the therapeutic window of most TPs is narrow. Excessive doses can cause multisystem damage and other diseases, even death . It is reported that the bioactive components in TWHF also has a strong toxicity .…”

Section: Introductionmentioning

confidence: 99%

Separation and simultaneous determination of seven bioactive components in Tripterygium wilfordii Hook. F. and Tripterygium preparations by micellar electrokinetic capillary chromatography

Guo

Wang

et al. 2018

Electrophoresis

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A simple, comprehensive, and highly selective MEKC method has been developed for simultaneous analysis of seven bioactive components (triptolide, wilfortrine, wilfordine, wilforgine, wilforine, triptophenolide, and triptonide) in the root extracts of Tripterygium wilfordii Hook. F. (TWHF) and Tripterygium preparations (TPs). Optimal BGE consisted of 10 mM sodium tetraborate, 30 mM SDS, and 30% v/v methanol. The separation voltage was 20 kV and the temperature was 25°C. A DAD was used and the detection wavelength was at 218 nm. Under the optimum conditions, the baseline separation of seven components was achieved in less than 26 min. Excellent precision, good stability, and accuracy were obtained. For all analytes, linear calibrations were established within 10–100 μg/mL. The LOD and LOQ were within 1.2–4.2 μg/mL and 4.0–14 μg/mL, respectively. The developed method was suitable for the determination of key components in TWHF and TPs.

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“…Studies have shown that tripterygium glycoside tablets have significant therapeutic effects on nephrotic syndrome, IgA nephropathy, and chronic glomerulonephritis, but it is often accompanied by frequent adverse reactions [5]. Tripterygium glycoside tablets exert significant toxic effects on the reproductive system of male SD rats, and the toxicity is time-and dose-dependent [16,17]. Furthermore, tripterygium glycoside tablets lead to acute hepatic injury, myocardial damage, and gastrointestinal inflammatory changes [18,19].…”

Section: Discussionmentioning

confidence: 99%

Subacute combined degeneration of the spinal cord is associated with tripterygium glycoside tablet usage

et al. 2019

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Background Subacute combined degeneration (SCD) is a neurodegenerative disease caused by vitamin B 12 deficiency. The lesions mainly involve the posterior cord, lateral cord, and peripheral nerves. Occasionally, the lesions also involve brain white matter and optic nerves in severe cases. Reports of drug-induced impaired absorption and metabolism of vitamin B 12 resulting in SCD are scarce. Introduction A patient developed SCD after long-term use of tripterygium glycoside tablets in the treatment of glomerulonephritis. However, after discontinuation and vitamin B 12 treatment with tripterygium glycoside tablet, the symptoms of SCD were significantly resolved. Conclusion Drug-induced SCD is a less commonly reported cause of the disease. Tripterygium glycoside tablets can induce adverse reactions in the digestive system, causing damage to absorption and metabolism of vitamin B 12. Physicians should be aware of the possibility of tripterygium glycoside tablet-induced SCD after excluding more common causes such as inadequate dietary intake and impaired absorption due to gastrointestinal diseases or genetic disorders.

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Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats

Cited by 35 publications

References 29 publications

Wuzi Yanzong Pill—Based on Network Pharmacology and In Vivo Evidence—Protects Against Spermatogenesis Disorder via the Regulation of the Apoptosis Pathway

Wuzi Yanzong Pill—Based on Network Pharmacology and In Vivo Evidence—Protects Against Spermatogenesis Disorder via the Regulation of the Apoptosis Pathway

Separation and simultaneous determination of seven bioactive components in Tripterygium wilfordii Hook. F. and Tripterygium preparations by micellar electrokinetic capillary chromatography

Subacute combined degeneration of the spinal cord is associated with tripterygium glycoside tablet usage

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