Toxicity Test LC<sub>50</sub> of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Methode

Rasyid, Maya Indra; Yuliani, Hilka; Angraeni, Lia

doi:10.1088/1755-1315/515/1/012052

Cited by 7 publications

(10 citation statements)

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“…The results of the data were transformed into the probit analysis to determine the lethal concentration 50 (LC 50 ) value of the extract. Calculation reference to Rasyid et al (2020) was determined by looking at the percent of individual deaths in each tube, as follows:…”

Section: Brine Shrimp Lethality Test (Bslt)mentioning

confidence: 99%

“…Furthermore, LC 50 values were measured at a concentration of 5,221 ppm. According to Rasyid et al (2020), the value of the toxicity of secondary metabolites of plants if LC 50 ≤ 30 ppm is very toxic, extracts with a value of 31 ≤ LC 50 ≤ 1,000 ppm are toxic, whereas if the LC 50 > 1,000 ppm this means nontoxic. The LC 50 obtained in this study is nontoxic.…”

Section: Eaesl-mediated Toxicity On a Salina Shrimp Larvaementioning

confidence: 99%

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Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Rahayu¹,

Chang²,

Supiyani³

et al. 2023

J App Pharm Sc

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Cancer is a malignant tumor with characteristics of uncontrolled cell growth, poor metastasis, leading to the dysfunction of organs and death of patients. Many efforts have been made, including the exploration of natural products. The purpose of this study was to determine the cytotoxicity of the Dillenia suffruticosa leaf extract against the cell lines MCF-7 and HepG2. The method used is an experimental research design with a completely randomized design. The treatment group consisted of the leaf extract of 500, 1,000, 2,000, 5,000, and 9,000 ppm for the brine shrimp lethality test (BSLT). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test used 10, 15, 20, 25, and 50 ppm concentrations and control (without extract). The data on the BSLT test was determined by analyzing the lethal concentration 50 (LC 50 ) value using the probit analysis, while the MTT test was determined by analyzing the IC 50 and analysis of variance test. The BSLT test results showed an LC 50 value of 5,221 ppm, which means that the leaf extract is not toxic. Simpor leaf extract at 25 ppm with IC50 of 88.52 was seen as the best concentration for liver cancer treatment. It can be concluded that D. suffruticosa shows cytotoxicity against the cell lines MCF-7 and HepG2 and can be used as a candidate for breast and liver cancer treatment.

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Section: Brine Shrimp Lethality Test (Bslt)mentioning

confidence: 99%

Section: Eaesl-mediated Toxicity On a Salina Shrimp Larvaementioning

confidence: 99%

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Rahayu¹,

Chang²,

Supiyani³

et al. 2023

J App Pharm Sc

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“…It is because the concentration of the test extract shows the number of toxic compounds. The mortality rate of larvae was caused by the extract that given against A. salina larvae (Rasyid et al, 2020). No dead larvae were found in the control, meaning there is no toxic sample extract in its vial while the tested fraction is toxic, hence, the complete test results can be seen in Table 2.…”

Section: Anti-cancer Activity Testmentioning

confidence: 99%

“…is the value that shows the concentration of toxic compounds causing organism mortality up to 50%. LC50 focuses on the total mortality of tested animal, therefore, the LC50 is used in the short-term test, because A. salina Leach larvae has simple digestion system (Rasyid et al, 2020). The toxicity level of the extract was categorized as follows: LC50 ≤ 30 mg/L = Very toxic; LC50 ≤ 1.000 mg/L = Toxic; LC50 > 1.000 mg/L = Non-toxic (Meyer et al, 1982).…”

Section: Anti-cancer Activity Testmentioning

confidence: 99%

Anti-cervical Cancer Test of the Ethyl Acetate Fraction of Terminalia catappa Linn Fruit Flesh against HeLa Cells

Chasani

Widyaningsih

Rastuti

et al. 2022

molekul

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The ethyl acetate fraction from methanol extract of ketapang (Terminalia catappa Linn) fruit flesh has anti-cancer potential. Therefore, this study aims to obtain the active anti-cervical cancer agent from the ethyl acetate fraction of ketapang fruit flesh’s methanol extract. The first step carried out was fractionation of the ethyl acetate fraction of methanol extract using gravity column chromatography with an eluent of ethyl acetate: chloroform: glacial acetic acid (1:8:1). The second step was a toxicity test on fractions produced from column chromatography. The third step was the test of secondary metabolite and anti-cancer activity of fraction possessing the highest toxicity to Artemia salina Leach. Fractionation of the concentrated ethyl acetate fraction by column chromatography, yielded seven subfractions (F1-F7). Furthermore, the cytotoxic test on A. salinaLeach shrimp larvae generated the following LC50 data from the 1st to 7th subfraction in a row, namely 566.2814 ppm, 87.9077 ppm, 216.6232 ppm, 566.2814 ppm, 560, 6647 ppm, 279.9213 ppm, and 194.6674 ppm. The most active subfraction is fraction 2 which have two groups of positive compounds, i.e. phenolics and saponins were obtained from the secondary metabolite test. Data from the infrared spectroscopy performed showed the presence of groups –OH, C-H aromatic, C=O carbonyl, C=C aromatic, C=C alkene, C-H aliphatic, C-H alkane, and C-O. The value of the anti-cancer activity of fraction 2 was IC50 = 165.37 ppm, which was included in the fairly active category as an anti-cervical cancer agent.

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“…The BSLT method was based on an extract's toxicity test, which was simple, fast, inexpensive, and accurate 38 . The LC50 toxicity test used a sample of Artemia salina leach larvae at extract concentrations of 0, 10, 100, 250, 500, and 750 ppm (Figure 5).…”

Section: Toxicological Evaluation Brine Shrimp Lethality Assaymentioning

confidence: 99%

Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

et al. 2021

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Meistera chinensis is one of the new generations of the Zingiberaceae family. Zingiberaceae have a large number of species and still need research to reveal their chemical content. Meistera chinensis, a local plant that is widespread in Konawe Regency, Southeast Sulawesi. Empirically, M. chinensis is used as a flavor enhancer in food, aches, and increases body immunity. There are no reports of chemical content or its biological activity. In this study, the fruit was tested for phytochemicals, antioxidants using the ABTS test, total phenolic, total flavonoid content, and toxicity test with BSLT. Meistera chinensis was extracted with ethanol and fractionated by ethyl acetate solvent use vacuum liquid chromatography. Phytochemical screening was carried out qualitatively by using the calorimetric method. The fraction toxicity was monitored by a lethal test for brine shrimp (BSLT). These fractions for the ABTS method obtained fractions 1-8 (F1-F8) and ascorbic acid were used as controls. The results showed radical scavenging activities fraction of M. chinensis fruit was a very strong activity with IC50 of 42.7±3.53 mg/L (F8). The total phenolic and flavonoid contents were 30.72±1.07 mgGAE/g and 8.02±0.48 mgQE/g, respectively. The phytochemical evaluation contains terpenoids, saponins, phenolics, steroids, alkaloids, and flavonoids. The BSLT toxicity test was found to be very toxic with IC50 of 5.20±0.72 mg/L. These findings indicate that the fruit of M. chinensis acts as an antioxidant and toxicity agent.

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Toxicity Test LC₅₀ of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Methode

Cited by 7 publications

References 1 publication

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Anti-cervical Cancer Test of the Ethyl Acetate Fraction of Terminalia catappa Linn Fruit Flesh against HeLa Cells

Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

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Toxicity Test LC50 of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Methode

Cited by 7 publications

References 1 publication

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Anti-cervical Cancer Test of the Ethyl Acetate Fraction of Terminalia catappa Linn Fruit Flesh against HeLa Cells

Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

Contact Info

Product

Resources

About

Toxicity Test LC₅₀ of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Methode