Meistera chinensis is one of the new generations of the Zingiberaceae family. Zingiberaceae have a large number of species and still need research to reveal their chemical content. Meistera chinensis, a local plant that is widespread in Konawe Regency, Southeast Sulawesi. Empirically, M. chinensis is used as a flavor enhancer in food, aches, and increases body immunity. There are no reports of chemical content or its biological activity. In this study, the fruit was tested for phytochemicals, antioxidants using the ABTS test, total phenolic, total flavonoid content, and toxicity test with BSLT. Meistera chinensis was extracted with ethanol and fractionated by ethyl acetate solvent use vacuum liquid chromatography. Phytochemical screening was carried out qualitatively by using the calorimetric method. The fraction toxicity was monitored by a lethal test for brine shrimp (BSLT). These fractions for the ABTS method obtained fractions 1-8 (F1-F8) and ascorbic acid were used as controls. The results showed radical scavenging activities fraction of M. chinensis fruit was a very strong activity with IC50 of 42.7±3.53 mg/L (F8). The total phenolic and flavonoid contents were 30.72±1.07 mgGAE/g and 8.02±0.48 mgQE/g, respectively. The phytochemical evaluation contains terpenoids, saponins, phenolics, steroids, alkaloids, and flavonoids. The BSLT toxicity test was found to be very toxic with IC50 of 5.20±0.72 mg/L. These findings indicate that the fruit of M. chinensis acts as an antioxidant and toxicity agent.

Sampo ekstrak kubis kombinasi daun pandan wangi adalah sediaan kosmetik yang dibuat dari bahan dasar ekstrak kubis dan daun pandan wangi serta penambahan bahan lain yang dapat digunakan pada kulit kepala tanpa menimbulkan iritas. Ekstrak kubis dan daun pandan wangi memiliki senyawa yang berkhasiat menghambat pertumbuhan ketombe pada kulit kepala , penelitian ini bertujuan untuk membuat sediaan sampo antiketombe dari ekstrak kubis kombinasi daun pandan wangi yang dapat memenuhi uji evaluasi fisik sediaan. Penelitian ini dilakukan secara eksperimental dengan beberapa evaluasi fisik sediaan diantaranya yaitu uji organoleptik, berdasarkan warna, bentuk dan aroma sediaan, uji pH, uji tinggi busa, uji homogenitas, dan uji viskositas. Hasil penelitian menunjukan formula sampo antiketombe ekstrak kubis dan daun pandan wangi hasilnya menunjukka bahwa uji evaluasi fisik sediaan sampo antiketombe ekstrak kubis kombinasi daun panan wangi memenuhi syarat secara fisik, pada uji organoleptik warna yang dihasilkan dari ketiga formula yaitu formula A, formula B dan formula C berwarna hijau gelap disebabkan oleh zat aktif yang digunakan yaitu daun pandan wangi, aroma yang dihasilkan adalah aroma dari penambahan pewangi yang digunakan dalam sediaan, pewangi yang digunakan yaitu minyak mawar serta bentuk dari sediaan yaitu kental. Pada uji pH ketiga formulasi telah memenuhi syarat mutu pH kulit kepala yaitu 4,5 – 6,5. Formula yang dihasilkan menghasilkan tinggi busa yang baik. Sediaan sampo yang dibuat dari ketiga formula homogen dan kental.

INTISARI   Bintang laut bertanduk merupakan salah satu biota laut yang digunakan sebagai obat tradisional. Salah satunya adalah pengobatan infeksi. Infeksi merupakan salah satu penyebab penyakit yang sering terjadi di daerah yang beriklim tropis khususnya Indonesia. Salah satu infeksi yang sering terjadi adalah infeksi pada kulit yang disebabkan oleh bakteri Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol bintang laut bertanduk dapat menghambat pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental yang terdiri atas lima perlakuan dan tiga kali pengulangan dengan menggunakan metode pengujian paper disk. Sampel uji yang diteliti adalah bintang laut bertanduk (Protoreaster nodosus) yang dilarutkan dalam DMSO 10% hingga didapatkan varian konsentrasi 5%, 10%, dan 15% kemudian dilakukan uji daya hambat terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak bintang laut bertanduk menghambat pertumbuhan bakteri Staphylococcus aureus masing-masing konsentrasi 5%, 10% dan 15% yakni 5,99mm, 6,69mm dan 7,64mm. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bintang laut bertanduk(Protoreaster nodosus) dapat menghambat pertumbuhan bakteri Staphylococcus aureus secara signifikan.  Kata kunci : Zona Hambat,  Bintang Laut Bertanduk, Staphylococcus aureus.   ABSTRACT  Horned starfish are one of the marine biota used as traditional medicine. One of them is the treatment of infections. Infection is one of the causes of diseases that often occur in the tropics, especially Indonesia. One infection that often occurs is an infection of the skin caused by Staphylococcus aureus. This study aims to determine whether the horned starfish ethanol extract can inhibit the growth of Staphylococcus aureus bacteria. This type of research used in this study is an experimental study consisting of five treatments and three repetitions using a diskette testing method. The test sample studied was a horned starfish (Protoreasternodosus) dissolved in DMSO 10% to 5%, 10%, and 15% of the variant of concentration was obtained then the inhibitory test was performed on Staphylococcus aureus bacteria. The results showed that the horned starfish extract inhibited the growth of Staphylococcus aureus, each concentrations of 5%, 10% and 15% respectively 5.99 mm, 6.69 mm and 7.64 mm. Based on these results it can be concluded that the ethanol extract of the horned starfish (Protoreasternodosus) can significantly inhibit the growth of Staphylococcus aureus bacteria. Keywords: Inhibited zone, Horn Star, Staphylococcus aureus.

Zingiberaceae has a large number of species in Indonesia. One of the newly discovered generations of this class is Meistera chinensis. It is widely distributed in Konawe Regency, Southeast Sulawesi. There is no information on the chemical compound and pharmacological activity of this plant. This study aims to identify chemical compounds, antibacterial, antioxidant, and antifungal of extract from Meistera chinensis fruit. Meistera chinensis fruit dry powder was extracted by maceration process using ethanol 96% solvent. The extract was concentrated using a rotary evaporator. The antioxidant activity was determined by the radical reduction test of 2,2-diphenyl-1-picrylhydrazyl (DPPH) with vitamin C and trolox as controls. The antimicrobial and antifungal activities were tested using agar diffusion method on Escherichia coli ACTT 35218, Staphylococcus aureus ACTT 25023, Streptococcus mutans ACTT 25675, and the fungus Candida albicans. The results showed that the phytochemical screening of Meistera chinensis fruit extracts contained saponins, terpenoids, steroids, alkaloids, phenolics, tannin, and flavonoids. The extracts exhibited antioxidant activity by scavenging DPPH radicals in a dose-dependent pattern with IC50 47.62 ± 2.93, 8.84 ± 0.69, 11.45 ± 0.87 for Meistera chinensis fruit, ascorbic acid, and trolox respectively. The results of the analysis of variance on antibacterial and antifungal properties showed that there was a significant difference (p = 0.00 < 0.05) against fungal and bacterial growth. Besade on the results, we make the following observations that Meistera chinensis fruit can be used as herbal medicine for the development of natural antioxidants, antibacterial, and antifungal.

Meistera chinensis is one of the endemic plants of Southeast Sulawesi from the Zingiberaceae family which has a large number of species and an interesting potential to be developed as a source medicine. However, there is no report on neither chemical contents nor biological activities of the plant. The aim of this study was to identify the phytochemicals by Thin-Layer Chromatography (TLC) and toxicity of Meistera chinensis fruit using the Brine Shrimp Lethality Test (BSLT). The fruit of Meistera chinensis was macerated with ethanol 95% for 3x24 h, and the solvent was evaporated in a vacuum. The toxicity of the extract was determined by the brine shrimp lethality test use with concentrations of 10, 100, 250, 500, and 750 μg / mL according of death of Artemia Salina larvae and calculated by determining the LC50 score. The results showed that the phytochemical screening by UV-Vis 254 nm and 366 nm indicates presence phenolics, flavonoids, steroids, terpenoids, alkaloids, and saponins. In the BSLT assay of ethanol extracts of the Meistera chinensis fruit were found highly toxic to the Artemia salina with IC50 values of 5.02 ± 1.11 mg / mL and control 2.26 ± 0.60 mg / mL. These findings indicate that the extract of Meistera chinensis fruit is very potential to be used as a medicinal plant and can be developed as a natural anticancer product..