Transfer of antibiotic resistance plasmids in pure and activated sludge cultures in the presence of environmentally representative micro-contaminant concentrations

Kim, Sungpyo; Yun, Zuwhan; Ha, Un-Hwan; Lee, Seokho; Park, Hongkeun; Kwon, Eilhann E.; Cho, Yunchul; Choung, Sungwook; Oh, Jun-Sik; Medriano, Carl Angelo; Chandran, Kartik

doi:10.1016/j.scitotenv.2013.08.100

Cited by 103 publications

(58 citation statements)

References 47 publications

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“…However, the persistence of the donor bacteria increased noticeably in non-aerated activated sludge microcosms when sulfamethoxazole or amoxicillin were applied at sub-inhibitory concentrations. Similar results were described by Kim et al, who found that ppb levels of tetracycline and sulfamethoxazole resulted in enhanced plasmid transfer frequencies in activated sludge [76]. Dr€ oge et al tested the potential of activated sludge concentrate to transfer conjugative plasmids to the 3-chlorobenzoate-degrading Pseudomonas sp.…”

Section: Monitoring Arb and Args In Wwtpssupporting

confidence: 60%

Antibiotic Resistance Elements in Wastewater Treatment Plants: Scope and Potential Impacts

Gatica

Kaplan

Cytryn

2015

The Handbook of Environmental Chemistry

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Antibiotic resistance is considered to be one of the most significant public health concerns of the twenty-first century. Although traditionally the propagation of antibiotic resistance was considered to be limited to hospitals and other clinical environments, there is a growing realization that it is also associated with anthropogenically impacted environmental reservoirs. Wastewater treatment plants are considered to be significant reservoirs of antibiotic resistance because they combine extremely high levels of fecal-and environmental-derived bacteria with residual concentrations of antibiotic compounds believed to induce selection. These bacteria are primarily congregated in dense biofilms that are "hot spots" for horizontal gene transfer, which can facilitate inter-and intraspecies transfer of antibiotic genes, potentially resulting in the development of multidrug-resistant strains. Several studies have demonstrated that although wastewater treatment plants significantly reduce bacterial concentrations, relatively high levels of antibiotic-resistant bacteria and resistance genes are still present in effluents released to aquatic and soil environments and that under certain circumstances these resistance elements may persist for long periods of time in downstream

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Section: Monitoring Arb and Args In Wwtpssupporting

confidence: 60%

Antibiotic Resistance Elements in Wastewater Treatment Plants: Scope and Potential Impacts

Gatica

Kaplan

Cytryn

2015

The Handbook of Environmental Chemistry

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“…Proper control experiments must be established to tease apart different effects of antibiotics. Without these data, reports may be misleading, such as studies suggesting antibiotics may promote the conjugation efficiency in pure and activated sludge cultures [92], and reduce the efficiency in sewage water [93]. Taken together, such reports suggest that HGT rates may differ depending on the environment.…”

Section: Quantifying Hgt In Complex Settingsmentioning

confidence: 99%

Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics

Lopatkin

Sysoeva

2016

BioEssays

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Horizontal gene transfer (HGT) is a major mechanism responsible for the spread of antibiotic resistance. Conversely, it is often assumed that antibiotics promote HGT. Careful dissection of the literature, however, suggests a lack of conclusive evidence supporting this notion in general. This is largely due to the lack of well-defined quantitative experiments to address this question in an unambiguous manner. In this review, we discuss the extent to which HGT is responsible for the spread of antibiotic resistance and examine what is known about the effect of antibiotics on the HGT dynamics. We focus on conjugation, which is the dominant mode of HGT responsible for spreading antibiotic resistance on the global scale. Our analysis reveals a need to design experiments to quantify HGT in such a way to facilitate rigorous data interpretation. Such measurements are critical for developing novel strategies to combat resistance spread through HGT.

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“…The characterization of these environmental isolates may provide information regarding the underlying mechanism favoring the successful environmental persistence of multidrug-resistant pathogens. Special attention has been given to wastewater due to the high abundances of ARB and ARGs deriving from human and animal feces and the presence of subtherapeutic antibiotic and heavy metal concentrations that favor the maintenance and proliferation of antimicrobial resistance in these aquatic ecosystems (11 – 13). Other human activities, such as international tourism and pilgrimage, further contribute to the spread of these emerging biological pollutants, as visitors might carry multiresistant infectious agents that can ultimately be disseminated via the local sewage system (14).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of NDM-Positive Escherichia coli from Municipal Wastewater in Jeddah, Saudi Arabia

Mantilla‐Calderon

Jumat

Wang

et al. 2016

Antimicrob Agents Chemother

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bThe emergence of resistance to last-resort antibiotics is a public health concern of global scale. Besides direct person-to-person propagation, environmental pathways might contribute to the dissemination of antibiotic-resistant bacteria and antibiotic resistance genes (ARGs). Here, we describe the incidence of bla NDM-1 , a gene conferring resistance to carbapenems, in the wastewater of the city of Jeddah, Saudi Arabia, over a 1-year period. bla NDM-1 was detected at concentrations ranging from 10 4 to 10 5 copies/m 3 of untreated wastewater during the entire monitoring period. These results indicate the ubiquity and high incidence of bla NDM-1 in the local wastewater. To track the bacteria carrying bla NDM-1 , we isolated Escherichia coli PI7, a strain of sequence type 101 (ST101), from wastewater around the Hajj event in October 2013. Genome sequencing of this strain revealed an extensive repertoire of ARGs as well as virulence and invasive traits. These traits were further confirmed by antibiotic resistance profiling and in vitro cell internalization in HeLa cell cultures. Given that this strain remains viable even after a certain duration in the sewerage, and that Jeddah lacks a robust sanitary infrastructure to fully capture all generated sewage, the presence of this bacterium in the untreated wastewater represents a potential hazard to the local public health. To the best of our knowledge, this is the first report of a bla NDM-1 -positive E. coli strain isolated from a nonnosocomial environment in Saudi Arabia and may set a priority concern for the need to establish improved surveillance for carbapenem-resistant E. coli in the country and nearby regions.

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Transfer of antibiotic resistance plasmids in pure and activated sludge cultures in the presence of environmentally representative micro-contaminant concentrations

Cited by 103 publications

References 47 publications

Antibiotic Resistance Elements in Wastewater Treatment Plants: Scope and Potential Impacts

Antibiotic Resistance Elements in Wastewater Treatment Plants: Scope and Potential Impacts

Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics

Isolation and Characterization of NDM-Positive Escherichia coli from Municipal Wastewater in Jeddah, Saudi Arabia

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