Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.

Pettersson, Bertil; Rippere, Karen E.; Yousten, Allan A.; Priest, Fergus G.

doi:10.1099/00207713-49-2-531

Cited by 81 publications

(41 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis yielded two major fatty acid types, namely, anteiso-C 15 and C 16 : 0 (Table 2). Straight-chain C 14 : 0 fatty acid was more common in the nematode-associated isolates than has been reported for most other Paenibacillus species (Shida et al, 1997a, b;Nakamura et al, 1996;Pettersson et al, 1999;Elo et al, 2001), an exception being P. borealis (Elo et al, 2001). While all the isolates shared a similar overall pattern, there were some differences between them in the actual levels of some fatty acids.…”

mentioning

confidence: 69%

See 1 more Smart Citation

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Enright

McInerney

Griffin

2003

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

NoteCharacterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. Endospore-forming bacteria were isolated from insect-pathogenic nematodes, Heterorhabditis spp., from three diverse geographical locations. Spindle-shaped sporangia of these bacteria adhere to the free-living infective stage of the nematode, which carries them to new insect hosts, where the bacterium reproduces. These isolates were characterized based on phenotypic and chemotaxonomic properties and 16S rRNA gene sequences. Analysis of the 16S rRNA gene placed the isolates within the genus Paenibacillus. The isolates shared higher sequence similarities with each other (95?1-100 %) than they did with any other named species within the genus (89?2-94 %). Paenibacillus macquariensis, Paenibacillus azoreducens, Paenibacillus amylolyticus and Paenibacillus durus were among the species with highest sequence similarity to these isolates. The isolates shared a high degree of phenotypic similarity and were easily distinguished from closely related members of the genus. Anteiso-C 15 : 0 and C 16 : 0 were among the major fatty acid types and the DNA G+C content was approximately 44 mol% in all isolates. DNA-DNA similarity studies revealed genomic heterogeneity among the isolates, such that they are likely to represent more than one species. Two of the isolates (both from a Heterorhabditis megidis isolate from Estonia) are phenotypically distinguishable from the others and are proposed as a single species, Paenibacillus nematophilus sp. nov. The type strain for this novel species is NEM1a T (=DSM 13559 T =NCIMB 13845 T ). The other isolates, although closely related to the proposed species, are likely to represent at least one, but most likely two, novel species.The genus Paenibacillus includes several species that are associated with dead insects in one way or another. Amongst these are obligate insect pathogens such as Paenibacillus popilliae, Paenibacillus lentimorbus and Paenibacillus larvae (Pettersson et al., 1999), as well as non-pathogens/facultative pathogens such as Paenibacillus apiarus (Nakamura, 1996) and Paenibacillus alvei (Krieg, 1981). Enright et al. (2001) found a Paenibacillus sp. associated with an insectpathogenic nematode, Heterorhabditis megidis EU17. This nematode was isolated during a survey conducted in Estonia (Griffin et al., 1999).Heterorhabditis spp. nematodes are lethal pathogens of insects. Infective juveniles (IJs), the only infective stage of these nematodes, carry in their intestine a symbiotic bacterium belonging to the genus Photorhabdus (Forst et al., 1997). Upon infection of a suitable host insect, the symbiont is released into the haemocoel and causes the death of the insect within 48 h (Poinar, 1990). The Photorhabdus sp. then completely colonizes the dead insect cadaver, assisting in the breakdown of the insect tissues by secretion of an array of enzymes (Forst & Nealson, 1996). It is upon this bacterium and the ...

show abstract

mentioning

confidence: 69%

“…Amongst these are obligate insect pathogens such as Paenibacillus popilliae, Paenibacillus lentimorbus and Paenibacillus larvae (Pettersson et al, 1999), as well as non-pathogens/facultative pathogens such as Paenibacillus apiarus (Nakamura, 1996) and Paenibacillus alvei (Krieg, 1981). Enright et al (2001) found a Paenibacillus sp.…”

mentioning

confidence: 99%

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Enright

McInerney

Griffin

2003

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…Strain P2-1 T was closely related to Paenibacillus cineris KCTC 3998 T , P. favisporus KCTC 3910 T and P. rhizosphaerae KCTC 13015 T with 96.5, 96.4 and 96.3 % gene sequence similarity, respectively Velázquez et al, 2004;Rivas et al, 2005c). The novel strain was related to P. lentimorbus ATCC 14707 T , P. thiaminolyticus JCM 8360 T and P. naphthalenovorans DSM 14203 T with gene sequence similarity values of 90.4-91.8 % (Shida et al, 1997;Pettersson et al, 1999;Daane et al, 2002). Strain P2-1 T showed low DNA-DNA relatedness values with P. cineris KCTC 3998 T (34.2 %), P. favisporus KCTC 3910 T (38.4 %), and P. rhizosphaerae KCTC 13015 T (24.9 %).…”

mentioning

confidence: 99%

“…Trees constructed by the NJ and MP methods for strain P2-1 T and all recognized species of the genus Paenibacillus are available as Supplementary Fig. S2 , 1997;Pettersson et al, 1999;Daane et al, 2002). Strain P2-1 T showed low DNA-DNA relatedness values with P. cineris KCTC 3998 T (34.2 %), P. favisporus KCTC 3910 T (38.4 %), and P. rhizosphaerae KCTC 13015 T (24.9 %).…”

mentioning

confidence: 99%

Paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from Thai soil

Akaracharanya¹,

Lorliam²,

Tanasupawat³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

A cellulolytic bacterium, strain P2-1 T , isolated from soil in Thailand, was characterized using a taxonomic approach based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, facultatively anaerobic, spore-forming and rod-shaped. It contained meso-diaminopimelic as the diagnostic diamino acid in the cell-wall peptidoglycan. The DNA G+C content was 52.7 mol%. The major isoprenoid quinone was MK-7. Anteiso-C 15 : 0 and iso-C 16 : 0 were the dominant cellular fatty acids. Phylogenetic analyses using the 16S rRNA gene sequence showed that the novel strain was affiliated to the genus Paenibacillus. Strain P2-1 T was closely related to Paenibacillus cineris KCTC 3998 T , P. favisporus KCTC 3910 T and P. rhizosphaerae KCTC 13015 T with 96.3-96.5 % gene sequence similarity. DNA-DNA relatedness, physiological characteristics and some biochemical characteristics clearly distinguished strain P2-1 T from related species of the genus Paenibacillus. Therefore, strain P2-1 T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus cellulositrophicus sp. nov. is proposed. The type strain is P2-1 T (5KCTC 13135 T 5PCU 305 T 5TISTR 1888 T ).The genus Paenibacillus as proposed by Ash et al. (1994) has undergone considerable changes as the number of recognized members of the genus has increased. At the time of writing, there were 94 recognized species in the genus. Members of the genus Paenibacillus have been isolated from very diverse environments such as soils, cattle faeces, dead honeybees, honeybee larvae, plant roots, food, warm springs, raw and heat-treated milk and from blood cultures (Berge et al., 2002;Chou et al., 2007;Roux & Raoult, 2004;Scheldeman et al., 2004;Shida et al., 1997; Velázquez et al., 2004). Some of these bacteria produce diverse assortments of extracellular polysaccharide-hydrolysing enzymes (Aÿ et al., 1998; Dasman et al., 2002;Zamost et al., 1991;Hespell, 1996;Lee et al., 2000;Morales et al., 1995;Nielsen & Sørensen, 1997). The cellulose-and xylan-degrading members of the genus Paenibacillus include the species P. barcinonensis, P. campinasensis, P. curdlanolyticus, P. favisporus, P. phyllosphaerae, P. xylanilyticus, P. panacisoli, P. siamensis, P. septentrionalis, P. montaniterrae, P. thailandensis and P. nanensis, as well as unidentified Paenibacillus spp. (Khianngam et al., 2009a, b;Ko et al., 2007;Pason et al., 2006;Rivas et al., 2005a Rivas et al., , b, c, 2006 Sánchez et al., 2005;Ten et al., 2006; Velázquez et al., 2004;Wang et al., 2008). In this paper, a novel cellulolytic bacterium isolated from Thai soil is described based on its phenotypic and chemotaxonomic characteristics and on 16S rRNA gene sequence analyses.A cellulolytic bacterial strain was isolated from a soil sample collected from Pua district in Nan province, in the northern part of Thailand. The novel strain was isolated using the spread plate method on CMC agar medium (l 21 : 1 g carboxymethylcellulose, 5 g peptone, 1 g yeast extr...

show abstract

“…jsp). Analysis of the sequence indicated that GPTSA 11 T contains the consensus signature sequence stretches PAEN 515F (59-GAGTAACTGCTCTCGGAATGACGGTACTTGA-GAAGAAAGCCCC-39) (Shida et al, 1997) and PAEN 862F (59-TCGATACCCTTGGTGCCGAAGT-39) (Pettersson et al, 1999), which are mostly found among different species within the genus Paenibacillus. Strain GPTSA 11 T showed the highest degree of sequence similarity with Paenibacillus apiarius DSM 5581 T (95?85 %), followed by Paenibacillus alvei IAM 1258 T (94?34 %), Paenibacillus cineris LMG 18439 T (93?87 %), Paenibacillus favisporus GMP 01 T (93?80 %), Paenibacillus chibensis JCM 11200 T (93?47 %) and Paenibacillus azoreducens CM1 T (93?40 %).…”

mentioning

confidence: 99%

Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India

Saha

Mondal

Mayilraj

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

Transfer of Bacillus lentimorbus and Bacillus popilliae to the genus Paenibacillus with emended descriptions of Paenibacillus lentimorbus comb. nov. and Paenibacillus popilliae comb. nov.

Cited by 81 publications

References 28 publications

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.

Paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from Thai soil

Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India

Contact Info

Product

Resources

About