Transformation of persimmon with a pear fruit polygalacturonase inhibiting protein (PGIP) gene

Brassica napus possesses an expanded set of polygalacturonase inhibitor protein genes that are differentially regulated in response to Sclerotinia sclerotiorum infection, wounding and defense hormone treatment

“…Over-expression of either leads to enhanced resistance (Ferrari et al 2003) whereas anti-sense expression increased disease symptoms (Ferrari et al 2006). Cotton, Gossypium hirsutum, (James and Dubery 2001) and pear, Pyrus communis, PGIPs (Tamura et al 2004) were shown to inhibit fungal PG activity. The latter also increased resistance to B. cinerea when expressed in transgenic tomato, Lycopersicon esculentum, (Powell et al 2000) and grapevine, Vitus vinifera, (Aguero et al 2005).…”

Spatial distribution of polygalacturonase-inhibiting proteins in Arabidopsis and their expression induced by Stemphylium solani infection

“…Total proteins were prepared by homogenizing tissues (2 mL/g tissue) in 2-5 volumes of 1 M NaCl, 0.1 M sodium acetate, pH 6.0, 1% polyvinylpyrrolidone-40, 0.2% NaBisulfate, and homogenates were incubated for 1 h at 4°C and centrifuged for 15 min at 15,000g (Tamuraa et al, 2004). The supernatant was precipitated with 2 volumes cold acetone.…”

Development of Agrobacterium-mediated transient transformation in persimmon (Diospyros kaki Thunb.)

“…The Agrobacterium-mediated genetic transformation of fruit crops was firstly developed in walnut (McGranahan et al, 1988) and then further applied to apricot, citrus, apple, etc., (Moore et al, 1992;Rugini et al, 1991;James et al, 1989). The persimmon genetic transformation by Agrobacterium-mediated has first been reported by Tao et al (1994) and was developed and optimized further (Tao et al, 1997;Gao et al, 2000Gao et al, , 2001Tamura et al, 2004). However, it was due to the higher phenolic content which can easily cause callus browning and the adventitious bud regeneration is genotype-dependent (Tao and Sugiura, 1992;Tetsumura and Yukinaga, 2000;Choi et al, 2001), the existing transformation system has been limited to few persimmon cultivars, such as 'Jirou'.…”