Transgenic Over-Expression of Bovine α-Lactalbumin and Human Insulin-Like Growth Factor-I in Porcine Mammary Gland

Donovan, Sharon M.; Monaco, Marcia H.; Bleck, Gregory T.; Cook, J.B.; Noble, M.S.; Hurley, W.L.; Wheeler, Matthew B.

doi:10.3168/jds.s0022-0302(01)70221-4

Cited by 11 publications

(10 citation statements)

References 46 publications

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“…Therefore, our laboratory created transgenic (Tg) swine with lactation-and mammary-specific over-expression of IGF-I in milk under the control of the bovine ␣-LA promoter. 121 The Tg sows produce milk containing 0.6 to 1.5 mg/L of colostrum and approximately 0.7 mg/L of mature milk, compared with 0.1 and 0.01 mg/L of the colostrum and mature milk in non-Tg sows. 121 The intestinal development of piglets suckling non-Tg and IGF-I Tg sows was compared at days 3, 7, 14, and 21 postpartum.…”

Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

“…121 The Tg sows produce milk containing 0.6 to 1.5 mg/L of colostrum and approximately 0.7 mg/L of mature milk, compared with 0.1 and 0.01 mg/L of the colostrum and mature milk in non-Tg sows. 121 The intestinal development of piglets suckling non-Tg and IGF-I Tg sows was compared at days 3, 7, 14, and 21 postpartum. 127 Piglets suckling IGF-I Tg sows had greater mucosal weight throughout the suckling period and longer villi and lactase and sucrase activity at day 7 and day 21 postpartum.…”

Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

“…The first approach is to transgenically overexpress a growth factor in milk. 120,121 The opposite approach is to remove or reduce the component in milk by gene knock out 122 or pharmacologic means. 123 For example, newborns of transforming growth factor-␤ (TGF-␤) knockout mice do not survive, but can be saved by oral administration of TGF-␤ or foster mothers providing TGF-␤ via the milk, providing evidence that milk-borne TGF-␤ is biologically active in the neonatal mouse.…”

Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

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Role of human milk components in gastrointestinal development: Current knowledge and future NEEDS

Donovan¹

2006

The Journal of Pediatrics

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Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

Section: Complexity Of Delineating the Role Of Human Milk Components mentioning

confidence: 99%

See 1 more Smart Citation

Role of human milk components in gastrointestinal development: Current knowledge and future NEEDS

Donovan¹

2006

The Journal of Pediatrics

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“…Transgenic pigs (TG) were generated by microinjection of pronuclear-stage porcine embryos using a bovine a-LA/human IGF-I gene construct as previously described (Bleck et al, 1998;Donovan et al, 2001). The gene construct contained approximately 2.0 kb of bovine a-LA 5¢ flanking region, the 2.0 kb a-LA coding region and 329 bp of the a-LA 3¢ flanking region.…”

Section: Production Of Transgenic Pigsmentioning

confidence: 99%

Mammary Specific Transgenic Over-expression of Insulin-like Growth Factor-I (IGF-I) Increases Pig Milk IGF-I and IGF Binding Proteins, with no Effect on Milk Composition or Yield

et al. 2005

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IGF-I regulates lactation by stimulating mammary mitogenesis, inhibiting apoptosis, and partially mediating the effects of growth hormone on lactogenesis. Herein, lactation performance during first and second parity was assessed in transgenic swine (TG) that over-expressed human IGF-I in milk under the control of the bovine alpha-lactalbumin promoter, regulatory regions and signal peptide coding sequence. Milk samples were collected throughout lactation (farrowing to d24) from TG sows and non-transgenic littermates (CON) and IGF-I, IGF-II, and IGFBP determined. Colostral (<24 h postpartum) IGF-I content was 26-fold greater (p<0.001) in TG sows (949+/- 107 microg/L; range 228-1,600 microg/L) than CON (36+/-17.8 microg/L) and was 50- to 90-fold greater (p< 0.001) in mature milk (d2-24 postpartum). There was no effect of parity on milk IGF-I content. Milk IGF-II concentration was unaffected by IGF-I over-expression. Low molecular weight IGFBP (IGFBP-2 and -5) in the milk of TG sows were higher (p=0.02) than CON in the early postpartum period, but did not differ in mature milk. Milk yield, determined by weigh-suckle-weigh, was similar in TG and CON as was litter weight gain. Milk nutrient composition was not significantly affected by IGF over-expression. Thus, mammary specific transgenic over-expression of IGF-I significantly increased milk IGF-I and IGFBP content, but did not impact lactation performance in swine.

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“…Transgenic swine overexpressing bovine α-lactalbumin had higher milk lactose content in the early lactation and 20%-50% greater milk yield on day 3, 6 and 9 of lactation than nontransgenic gilts, and weight gain of piglets sucking α-Lac gilts was greater from day 7 and 21 postpartum than control piglets [1] . It interacts with UDP-galactosyltransferase to form the lactose synthetase and thus might be a key protein for lactogenesis.…”

mentioning

confidence: 91%

An expression profile of human α-lactalbumin in the milk of transgenic mouse

Liu

Wei

et al. 2004

Sci. China Ser. C.-Life Sci.

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Five female transgenic mice were produced by microinjection using a construct made up of a 7.3-kb-5′ flanking region and a 2.0-kb coding region of human α-lactalbumin, as well as a 227-bp 3′ -flanking region from bovine growth hormone gene. A founder female expressed human α-lactalbumin as much as 0.3 g per liter of its milk, approximately a 3-fold increase in the total α-lactalbumin concentration of the transgenic mouse milk. Compared with the normal mice, the expression profile of the hα-Lac transgene in the transgenics is different during the lactation, showing low level in the first 3 days and becoming increased from day 4, then gradually reaching and stabilizing at the highest level from day 13. In addition, the milk yielding volume in the transgenics tended to be higher than in normal mice, suggesting higher concentrations of α-lactalbumin might boost more milk output.

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Transgenic Over-Expression of Bovine α-Lactalbumin and Human Insulin-Like Growth Factor-I in Porcine Mammary Gland

Cited by 11 publications

References 46 publications

Role of human milk components in gastrointestinal development: Current knowledge and future NEEDS

Role of human milk components in gastrointestinal development: Current knowledge and future NEEDS

Mammary Specific Transgenic Over-expression of Insulin-like Growth Factor-I (IGF-I) Increases Pig Milk IGF-I and IGF Binding Proteins, with no Effect on Milk Composition or Yield

An expression profile of human α-lactalbumin in the milk of transgenic mouse

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