Transport of viral specimens.

Johnson, F. Brent

doi:10.1128/cmr.3.2.120-131.1990

Cited by 13 publications

(16 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As observed in the current study, isolation of spumavirus from five of eight diluents after 7 days at 4°C, compared with one isolate (i.e., virus maintained in EMEM‐10%) at 23°C, concurs with the recognized protective effect of refrigeration [8,14]. Organic constituents in the media, such as sucrose or FBS (i.e., the sucrose component in CTM; the FBS supplement in EMEM), further served as viral stabilizing agents [15,16]. Increased survival of spumavirus in CTM, EMEM‐2%, and EMEM‐10% rather than in PBS, EMEM‐0%, or distilled water after 7 days, was in accord with the expected protective effect of medium organic load.…”

Section: Resultssupporting

confidence: 83%

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Lotlikar

Lipson

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

The persistence of a previously characterized spumavirus strain (strain SV-522) was investigated utilizing various laboratory media and waters, including Eagle's minimal essential medium (EMEM) plus 0% fetal bovine serum (EMEM-0%), EMEM-2%, EMEM-10%, Chlamydia transport medium (CTM), phosphate-buffered saline, distilled, estuarine, and marine water, human serum, and the germicides, ethyl alcohol (70%) and sodium hypochlorite (10%). Experiments were performed at 4 degrees C and/or 23 degrees C. Infectivity endpoints were determined in stock aliquots upon initiation of testing and then after 3, 5, 7, and 10 days. The virus was reisolated from all diluents after 5 days at 23 degrees C and in EMEM-10% after 7 days. The virus was detected in CTM, EMEM-2%, EMEM-10%, and estuarine and marine waters after 7 days at 4 degrees C. Differences in the persistence of the virus may be ascribed to temperature and organic load. Water ionic strengths (e.g., estuarine vs. marine water) had no effect on modifying persistence of viral particles. Infectivity of spumavirus was undetectable after 30 s in 70% ethanol or 10% sodium hypochlorite. After 30 min at 23 degrees C, spumavirus infectivity in normal but not heat-inactivated human serum increased by almost 100-fold. Persistence of infectivity of primate spumavirus after 7 days in media and waters, and the agent's infectious potential in the human host, emphasize a need for cautious recognition during the manipulation of primate cells/organs and in the handling of primates themselves.

show abstract

Section: Resultssupporting

confidence: 83%

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Lotlikar

Lipson

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…This reflects the pattern of virus shedding (8). Samples which can be detected as positive must contain a sufficient number of viable viral particles capable of replication (6,8,10,23,24), making it difficult or even impossible to isolate influenza viruses from samples taken at later stages of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…3-4 days after sampling), and high virus concentrations are important for diagnosis. However, influenza viruses can be successfully detected in the samples transported at ambient temperatures and analysed several days after the sampling (8,23).…”

Section: Discussionmentioning

confidence: 99%

“…Choosing the most appropriate diagnostic tool depends on the sensitivity and specificity of the tests, time to obtain results, repeatability, the simplicity of the procedure and costs (18). However, the effectiveness of a particular diagnostic method in the direct diagnostics of influenza is also affected by various factors such as the age of the patient (19)(20)(21), early sample collection and rapid transport to the laboratory (8,22,23). The aim of our study was to assess the effect of patients' age and time to confirm influenza infection using four diagnostic methods (virus isolation, rapid test, RT-PCR, and real-time RT-PCR).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Factors Affecting the Success of Influenza Laboratory Diagnosis

Kissová¹,

Svitok²,

Klement³

et al. 2014

Cent Eur J Public Health

View full text Add to dashboard Cite

Influenza is one of the most common human infectious diseases, and has profound health and economic consequences. The laboratory diag- nosis of influenza virus infections plays an important role in the global surveillance of influenza. Therefore, there is a growing demand for highly sensitive and rapid methods for detecting influenza. The performance of particular diagnostic methods is affected by various factors. In this study, we assess the effects of patients' age and time to diagnosis on the probability of detecting influenza using four diagnostic methods (virus isolation, rapid test, RT-PCR and real-time RT-PCR). We examined 3,546 samples from central and eastern Slovakia during the influenza seasons from 2005-2006 to 2010-2011. In general, the probability of influenza detection significantly decreased with the time from onset of illness to sample collection (T1) as well as with patients' age (AGE). On the contrary, time from sample collection to delivery (T2) did not play a role in the prob- ability of influenza detection. As judged by odds ratios, the virus isolation method was most sensitive to T1, followed by the rapid test and RT-PCR methods. For the effect of AGE, the rapid test and virus isolation methods were more sensitive than PCR-based methods. The effects of T1 and AGE were independent of each other. Laboratories which participate in inifluenza surveillance should use several methods to enable rapid and accurate influenza A and B virus detection.

show abstract

“…Статистический анализ данных осуществляли с помощью стандартных пакетов компьютерных программ «Excel» («Microsoft», США) и «Statistica 10» («StatSoft», США) и метода дисперсионного анализа (one-way, factorial и main effects ANOVA): определяли значимость различий между средними значениями и оценивали значимость влияния каждого из факторов на изменчивость признака. Различия считали статистически значимыми при p < 0,05 [5].…”

Section: A B C D E Funclassified

Infectious Activity of L. Pasteur Rabies Virus Vaccine Strain Frozen-Dried in Various Protective Media and Then Stored at Various Temperatures

Varianytsia

Vysekantsev

Pharmastandard-Biolik³

2018

Probl Cryobiol Cryomed

View full text Add to dashboard Cite

Реферат: В работе исследовали сохранность производственного вакцинного штамма вируса бешенства L. Pasteur после лиофилизации в различных защитных средах и последующего хранения при температурах 5,-20 и-80ºС. После хранения в течение 24 месяцев (срок наблюдения) наиболее высокие показатели инфекционной активности вируса во всех средах были отмечены при температуре-80ºС. Максимальную сохранность вируса обеспечивала защитная среда на основе питательной среды DMEM с добавлением 0,5% бычьего сывороточного альбумина, 1% желатина и 5% сахарозы. Инфекционная активность образцов, лиофилизированных в средах, содержащих 5% сахарозы или 3% желатина и 5% сахарозы, или 10% сахарозы, была значимо ниже. Установлено, что добавление 3% желатина в защитную среду значительно увеличивает время растворения препаратов по сравнению с другими средами и нормой регламента (не более минуты), что затрудняет их практическое использование. Время растворения образцов с 1% желатина и 5% сахарозы или 10% сахарозы было значимо больше, чем препаратов с 5% сахарозы, но соответствовало производственному регламенту. Полученные результаты свидетельствуют о необходимости подбора индивидуальных режимов сушки при лиофилизации вирусов в защитных средах с различным составом. Ключевые слова: вирус бешенства, лиофилизация, культура клеток, защитные среды, долгосрочное хранение, сохранность вируса, инфекционная активность вируса. Реферат: У роботі досліджували збереженість виробничого вакцинного штаму вірусу сказу L. Pasteur після ліофілізації в різних захисних середовищах та наступного зберігання за температур 5,-20 и-80ºС. Після зберігання протягом 24 місяців (термін спостереження) найбільш високі показники інфекційної активності вірусу в усіх середовищах були відзначені за температури-80ºС. Максимальну збереженість вірусу забезпечувало захисне середовище на основі живильного середовища DMEM із додаванням 0,5% бичачого сироваткового альбуміну, 1% желатину та 5% сахарози. Інфекційна активність зразків, ліофілізованих у середовищах, які містять 5% сахарози або 3% желатину і 5% сахарози, або 10% сахарози, була значуще нижчою. Встановлено, що додавання 3% желатину в захисне середовище значно збільшує час розчинення препаратів у порівнянні з іншими середовищами і нормою регламенту (не більше хвилини), що ускладнює їх практичне використання. Час розчинення зразків з 1% желатину і 5% сахарози або 10% сахарози був значуще більше, ніж препаратів з 5% сахарози, але відповідав виробничому регламенту. Отримані результати свідчать про необхідність підбору індивідуальних режимів сушки при ліофілізації вірусів у захисних середовищах з різним складом. Ключові слова: вірус сказу, ліофілізація, культура клітин, захисні середовища, довгострокове зберігання, збереженість вірусу, інфекційна активність вірусу.

show abstract

Transport of viral specimens.

Cited by 13 publications

References 43 publications

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Factors Affecting the Success of Influenza Laboratory Diagnosis

Infectious Activity of L. Pasteur Rabies Virus Vaccine Strain Frozen-Dried in Various Protective Media and Then Stored at Various Temperatures

Contact Info

Product

Resources

About