Tri‐mode optical biopsy probe with fluorescence endomicroscopy, Raman spectroscopy, and time‐resolved fluorescence spectroscopy

Wood, Harry A. C.; Ehrlich, Katjana; Yerolatsitis, Stephanos; Kufcsák, Andras; Quinn, Tom; Fernandes, Susan; Norberg, Dominic; Jenkins, Nia C.; Young, Vikki; Young, Irene; Hamilton, Katie; Seth, Sohan; Akram, Ahsan; Thomson, Robert R.; Finlayson, Keith; Dhaliwal, Kevin; Stone, James M.

doi:10.1002/jbio.202200141

Cited by 4 publications

(1 citation statement)

References 44 publications

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“…The absence of tools for real-time quantitative and objective assessment of CRC has prompted the development of new optical technologies that could assist in clinical decision-making. Examples include hyperspectral imaging[6-8], re ectance [9], auto uorescence [10], or Raman spectroscopy [11]. Among these, auto uorescence spectroscopy stands out as a promising technology due to its inherent sensitivity to functional and structural changes in tissues.…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens

Herrando,

Fernández,

Azevedo

et al. 2024

Preprint

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Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report a characterization of the ex vivo autofluorescence lifetime fingerprint of colorectal tissues obtained from 73 patients that underwent surgical resection. We specifically target the autofluorescence characteristics of collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing a fiber-based dual excitation (375 nm and 445 nm) optical imaging system. Autofluorescence-derived parameters obtained from normal tissues, adenomatous lesions, and adenocarcinomas were analyzed considering the underlying clinicopathological features. Our results indicate that differences between tissues are primarily driven by collagen and flavins autofluorescence parameters. We also report changes in the autofluorescence parameters associated with NAD(P)H that we tentatively attribute to intratumoral heterogeneity, potentially associated to the presence of distinct metabolic subpopulations. Changes in autofluorescence signatures of tumors were also observed with lymphatic and venous invasion, differentiation grade, and microsatellite instability. Finally, we characterized the impact of radiative treatment in the autofluorescence fingerprints of rectal tissues and observed a generalized increase in the mean lifetime of radiated tumors, which is suggestive of altered metabolism and structural remodeling. Overall, our preliminary findings indicate that multiparametric autofluorescence lifetime measurements have the potential to significantly enhance clinical decision-making in CRC, spanning from initial diagnosis to ongoing management. We believe that our results will provide a foundational framework for future investigations to further understand and combat CRC exploiting autofluorescence measurements.

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Section: Introductionmentioning

confidence: 99%

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens

Herrando,

Fernández,

Azevedo

et al. 2024

Preprint

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show abstract

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens

Herrando,

Fernandez,

Azevedo

et al. 2024

Sci Rep

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Colorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report a characterization of the ex vivo autofluorescence lifetime fingerprint of colorectal tissues obtained from 73 patients that underwent surgical resection. We specifically target the autofluorescence characteristics of collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing a fiber-based dual excitation (375 nm and 445 nm) optical imaging system. Autofluorescence-derived parameters obtained from normal tissues, adenomatous lesions, and adenocarcinomas were analyzed considering the underlying clinicopathological features. Our results indicate that differences between tissues are primarily driven by collagen and flavins autofluorescence parameters. We also report changes in the autofluorescence parameters associated with NAD(P)H that we tentatively attribute to intratumoral heterogeneity, potentially associated to the presence of distinct metabolic subpopulations. Changes in autofluorescence signatures of malignant tumors were also observed with lymphatic and venous invasion, differentiation grade, and microsatellite instability. Finally, we characterized the impact of radiative treatment in the autofluorescence fingerprints of rectal tissues and observed a generalized increase in the mean lifetime of radiated adenocarcinomas, which is suggestive of altered metabolism and structural remodeling. Overall, our preliminary findings indicate that multiparametric autofluorescence lifetime measurements have the potential to significantly enhance clinical decision-making in CRC, spanning from initial diagnosis to ongoing management. We believe that our results will provide a foundational framework for future investigations to further understand and combat CRC exploiting autofluorescence measurements. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-74224-8.

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Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Adams

Kufcsák

Ehrlich

et al. 2023

IEEE Trans. Biomed. Eng.

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Tri‐mode optical biopsy probe with fluorescence endomicroscopy, Raman spectroscopy, and time‐resolved fluorescence spectroscopy

Abstract: We present an endoscopic probe that combines three distinct optical fibre technologies including: A high-resolution imaging fibre for optical endomicroscopy,

Cited by 4 publications

References 44 publications

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging: a preliminary study on surgical specimens

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

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