Triacylglycerol analysis of partially hydrogenated vegetable oils by silver ion HPLC

Advances in Silver Ion Chromatography for the Analysis of Fatty Acids and Triacylglycerols—2001 to 2011

“…Heptane-acetonitrile and heptaneacetone were successfully applied to the separation of positionally isomeric TAG and TAG containing oleoyl-and elaidyl-moieties in partially hydrogenated vegetable oils. 39 Heptane-acetone phases were "too weak", however, to elute TAG with more than two double bonds in well shaped peaks.…”

Theory and Practice of UHPLC and UHPLC–MS

“…The second type is hexane-based mobile phases with the addition of acetonitrile as the polar modifier (Adlof and List, 2004;Adlof, 1994Adlof, , 1995Dugo et al, 2004Lísa et al, 2013). In addition to these two main types, some other solvent combinations have been also reported as well, such as toluene, hexane and ethylacetate , methanol and acetonitrile (van der Klift et al, 2008), and 13 different eluent systems containing hexane, heptane, or isooctane with the addition of acetonitrile, propionitrile or butyronitrile (Muller et al, 2006), heptane with acetonitrile or acetone (Macher and Holmqvist, 2001), or acetone with acetonitrile (NikolovaDamyanova et al, 1995a,b). Hexaneeacetonitrile mobile phases have a unique property of possible regioisomeric resolution of unsaturated TG, which has not been reported for chlorinated mobile phases.…”

“…The retention behavior observed in our experiments in hexanee2-propanoleacetonitrile mobile phases (Lísa et al, 2009a,b;) is mostly in a good agreement with published data with few exceptions. In mobile phases consisting of hexaneeacetonitrile (Adlof, 1995;Dugo et al, 2004), heptaneeacetonitrile (Macher and Holmqvist, 2001), or hexanee2-propanoleacetonitrile (Lísa et al, 2009a,b;, the retention order of all TG follows the rule that an increasing number of DB means longer retention, while one exception is reported above for DMoMo (4) < DDSa (4) SaSaT (3). The explanation of changed retention patterns probably lies in the different mobile-phase composition, which affects the retention mechanism.…”

“…Chlorinated solvents have been used in numerous works reporting excellent overall separation, but we have not found any papers reporting the regioisomeric resolution. Almost all papers (Adlof and List, 2004;Adlof, 1995;Dugo et al, 2004Macher and Holmqvist, 2001;Lísa et al, 2009a,b;Adlof, 2007;Smith et al, 1981) published on the regioisomeric resolution of TG are based on mobile phases containing acetonitrile in hexane (or heptane), except for one work measured on a silver complexation column in toluene, hexane, and ethylacetate . When acetonitrile is replaced by other solvents (e.g., 2-propanol, ethanol, and ethylacetate) (Muller et al, 2006;Lísa et al, 2009a,b), peak shapes and the theoretical number of plates may even be increased for certain compositions, but the regioisomeric resolution is lost or at least dramatically reduced.…”

Quantitation of triacylglycerols in edible oils by off-line comprehensive two-dimensional liquid chromatography–atmospheric pressure chemical ionization mass spectrometry using a single column

“…However, the NARP system has a low selectivity for the separation of TAGs with the same ECN. The silver-ion HPLC system is based on the formation of weak silver ion complexes with the -electrons of the DBs present in the TAGs [10][11][12][13]. TAGs are separated according to the number and position of the DBs.…”