TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

Castiglioni, Sara; Romeo, Valentina; Locatelli, Laura; Cazzaniga, Alessandra; Maier, Jeanette A. M.

doi:10.1038/s41598-018-34324-8

Cited by 24 publications

(29 citation statements)

References 45 publications

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“…Here, we confirm the relevant role of ROS, since exposure for a short time to low concentrations of H 2 O 2 suffices to upregulate RUNX2 even in the absence of the osteogenic cocktail. Indeed, ROS are fundamental in promoting cell differentiation partly through autophagy, which is emerging as the most potent accelerator of matrix mineralization and bone homeostasis [75]. Interestingly, we did not observe a modulation of ROS in bMSCs cultured in high-Mg conditions.…”

Section: Discussioncontrasting

confidence: 65%

Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D3

Mammoli

Castiglioni

Parenti

et al. 2019

IJMS

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Magnesium (Mg) is crucial for bone health. Low concentrations of Mg inhibit the activity of osteoblasts while promoting that of osteoclasts, with the final result of inducing osteopenia. Conversely, little is known about the effects of high concentrations of extracellular Mg on osteoclasts and osteoblasts. Since the differentiation and activation of these cells is coordinated by vitamin D3 (VD3), we investigated the effects of high extracellular Mg, as well as its impact on VD3 activity, in these cells. U937 cells were induced to osteoclastic differentiation by VD3 in the presence of supra-physiological concentrations (>1 mM) of extracellular Mg. The effect of high Mg concentrations was also studied in human bone-marrow-derived mesenchymal stem cells (bMSCs) induced to differentiate into osteoblasts by VD3. We demonstrate that high extra-cellular Mg levels potentiate VD3-induced osteoclastic differentiation, while decreasing osteoblastogenesis. We hypothesize that Mg might reprogram VD3 activity on bone remodeling, causing an unbalanced activation of osteoclasts and osteoblasts.

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Section: Discussioncontrasting

confidence: 65%

Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D3

Mammoli

Castiglioni

Parenti

et al. 2019

IJMS

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“… 62 , 63 Otherwise, some studies have reported that Mg ions can stimulate the proliferation of BMSCs and promote BMSCs differentiation into osteoblasts through regulation of transient receptor potential melastatin 7 (TRPM7) channel activity and acceleration of notch intracellular domain translocation. 64 – 66 It is worth mentioning that we did not detect the serum magnesium ion concentration, which can cause cardiovascular, renal, liver disease, as well as metabolism disorder if present in higher concentrations. 67 However, not only all the experimental animals were healthy before being sacrificed, but also there was no obvious inflammatory cell infiltration in H&E staining of bone tissue, which proved that all the composite scaffold has good biocompatibility.…”

Section: Discussionmentioning

confidence: 84%

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Meng

et al. 2020

J Tissue Eng

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Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

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“…This result is in agreement with data from cultured cells. Indeed, both TRPM7 or MagT1 silencing and Mg 2+ deficiency activate autophagy in human mesenchymal stem cells induced osteogenic differentiation [39]. Consistently, high concentrations of extracellular Mg 2+ inhibit autophagy in chondrocyte ATDC5 cells [40].…”

Section: Discussionmentioning

confidence: 89%

Magnesium Deficiency Alters Expression of Genes Critical for Muscle Magnesium Homeostasis and Physiology in Mice

et al. 2021

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Chronic Mg2+ deficiency is the underlying cause of a broad range of health dysfunctions. As 25% of body Mg2+ is located in the skeletal muscle, Mg2+ transport and homeostasis systems (MgTHs) in the muscle are critical for whole-body Mg2+ homeostasis. In the present study, we assessed whether Mg2+ deficiency alters muscle fiber characteristics and major pathways regulating muscle physiology. C57BL/6J mice received either a control, mildly, or severely Mg2+-deficient diet (0.1%; 0.01%; and 0.003% Mg2+ wt/wt, respectively) for 14 days. Mg2+ deficiency slightly decreased body weight gain and muscle Mg2+ concentrations but was not associated with detectable variations in gastrocnemius muscle weight, fiber morphometry, and capillarization. Nonetheless, muscles exhibited decreased expression of several MgTHs (MagT1, CNNM2, CNNM4, and TRPM6). Moreover, TaqMan low-density array (TLDA) analyses further revealed that, before the emergence of major muscle dysfunctions, even a mild Mg2+ deficiency was sufficient to alter the expression of genes critical for muscle physiology, including energy metabolism, muscle regeneration, proteostasis, mitochondrial dynamics, and excitation–contraction coupling.

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TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

Cited by 24 publications

References 45 publications

Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D3

Magnesium Is a Key Regulator of the Balance between Osteoclast and Osteoblast Differentiation in the Presence of Vitamin D3

Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Magnesium Deficiency Alters Expression of Genes Critical for Muscle Magnesium Homeostasis and Physiology in Mice

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