Turbo methanol extract inhibits bone resorption through regulation of T cell function

Balakrishnan, Babita; Indap, Madhavi; Singh, Surya Pratap; Krishna, C. Murali; Chiplunkar, Shubhada V.

doi:10.1016/j.bone.2013.10.008

Cited by 6 publications

(7 citation statements)

References 39 publications

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“…Estrogen deficiency in menopausal women leads to the common form of osteoporosis known as postmenopausal osteoporosis. The decline in estrogen level not only affects osteoclastogenesis and osteoblastogenesis but also regulates the activity of T cells (5, 6). Receptor activator of the nuclear factor kappa B Ligand (RANKL) and Macrophage- Colony Stimulating Factor (M-CSF) are essential key factors produced by T cells and osteoblasts that promote osteoclasts proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…displayed a potent suppressive effect on osteoclast differentiation without any cytotoxicity (13). Earlier work from our group has shown that a mollusk Turbo brunneus can inhibit bone resorption by regulating T cell activity (6). Based on this background, we have further investigated the anti-osteoclastogenic effect of Hexane fraction derived from the crude methanolic extract of Turbo brunneus (TME) and its mechanism of action in osteoclast related RANK-RANKL pathway.…”

Section: Introductionmentioning

confidence: 99%

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Hexane Fraction of Turbo brunneus Inhibits Intermediates of RANK-RANKL Signaling Pathway and Prevent Ovariectomy Induced Bone Loss

et al. 2019

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Osteoporosis is a “silent disease” characterized by fragile and impaired bone quality. Bone fracture results in increased mortality and poor quality of life in aged people particularly in postmenopausal women. Bone is maintained through the delicate balance between osteoclast-mediated bone resorption and osteoblast-mediated bone formation. The imbalance is caused most often by overly active osteoclasts due to estrogen deficiency. Natural products have long been used to prevent and treat osteoporosis since they have fewer side effects. The marine environment is a potential source of biologically and structurally novel biomolecules with promising biological activities but is less explored for the treatment of bone-related diseases. The present study aims to evaluate the antiosteoporotic effect of Hexane fraction of Turbo brunneus methanolic extract (HxTME) and to investigate its role in RANK-RANKL signaling pathway using in vitro osteoclasts cultures and in vivo ovariectomized (OVX) Swiss mice model. The present study demonstrated that the HxTME significantly inhibited RANKL induced osteoclast differentiation and maturation in vitro. HxTME completely downregulated the mRNA expression of key transcription factors such as NFATc1, c-FOS, and osteoclasts related genes involved in osteoclastogenesis. In vivo studies also depicted the effectiveness of HxTME in ovariectomized mice by preserving bone microarchitecture, mineral content, and inhibiting bone loss in treated mice as analyzed by Histomorphometry, MicroCT, and Raman spectroscopy. Oral administration of HxTME fraction resulted in the decreased percentage of F4/80+, CD11b+, and CD4+ RANKL+ T cells in OVX mice whereas pro-osteoclastic cytokine, IL6 was markedly reduced upon treatment with HxTME. On stimulation with PMA/Io and PHA, a significant decrease in proliferative response in the splenocytes of HxTME treated OVX mice was observed. Fatty acid profiling revealed that HxTME is rich in ω3 and ω6 polyunsaturated fatty acids (PUFAs), which have high nutraceutical properties and are known to play important role in growth, development and maintenance of health. Therefore, HxTME may be a good source of nutraceutical in the treatment of bone-related diseases particularly in postmenopausal osteoporosis and may be pursued as a potential candidate for treatment and management of osteoporosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hexane Fraction of Turbo brunneus Inhibits Intermediates of RANK-RANKL Signaling Pathway and Prevent Ovariectomy Induced Bone Loss

et al. 2019

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“…Estrogen deficiency leads to an increase in the immune function, which augments T cell activation [ 35 ]. Balakrishnan et al reported that lymphocytes from mice in an OVX group showed higher expressions of CD3, CD4, and CD8 than sham-operated controls [ 36 ]. Dendritic cells (DCs) are the most powerful antigen-presenting cells, and OVX induces the activation of both CD4 + and CD8 + cells by antigen presentation of DCs.…”

Section: Discussionmentioning

confidence: 99%

Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract ofHovenia dulcis) on the Ovariectomized Mice

Hwang

Kim

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Protective effect of new oriental medicine (Kyungokgo mixed with water extract of Hovenia dulcis, KOGHD) was assessed on the bone loss induced mice by ovariectomy. In the in vivo experiments, antiosteoporosis effect of KOGHD was investigated using ovariectomized osteoporosis mice model. After 6 weeks of treatment, the mice were euthanized, and the effect of Kyungokgo (KOG) and KOGHD on body weight, spleen weigh, thymus weight, uterine weight, serum biochemical indicators, bone weight and length, immune cell population, bone morphometric parameters, and histological stains was observed. Our results showed that KOGHD prevented the deterioration of trabecular microarchitecture caused by ovariectomy, which were accompanied by the lower levels of bone turnover markers and immune cell population as evidenced by the inhibition of RANKL-mediated osteoclast differentiation without cytotoxic effect on bone marrow derived macrophages (BMMs). Therefore, these results suggest that the Hovenia dulcis (HD) supplementation in the KOG may also prevent and treat bone loss.

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“…rigida which are unique 24 member-macrolides showed antiosteoporotic activity by inhibiting osteoclasts [37]. Our earlier investigation has shown that mollusc Turbo brunneus inhibits the bone resorption by acting on T cells to reduce the TNF α secretion which is responsible for osteoclast maturation and also revealed a potent antiosteoclastogenic activity [38]. …”

Section: Discussionmentioning

confidence: 99%

Methanol Extract ofEuchelus asperPrevents Bone Resorption in Ovariectomised Mice Model

Balakrishnan¹,

Chiplunkar

Indap³

2014

Journal of Osteoporosis

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Marine molluscs are widely distributed throughout the world and many bioactive compounds exhibiting antiviral, antitumor, antileukemic, and antibacterial activity have been reported worldwide. The present study was designed to investigate the beneficial effect of methanol extract of Euchelus asper (EAME) on estrogen deficiency induced osteoporosis in ovariectomised mice model. Forty-two female Swiss albino mice were randomly assigned into Sham operated (Sham) group and six ovariectomised (OVX) subgroups such as OVX with vehicle (OVX); OVX with estradiol (2 mg/kg/day); OVX with EAME of graded doses (25, 50, 100, and 200 mg/kg/day). Bone turnover markers like serum alkaline phosphatase (ALP), serum acid phosphatase (ACP), serum calcium, and histological investigations of tibia and uterus were analysed. Metaphyseal DNA content of the femur bone was also studied. Antiosteoclastogenic activity of EAME was examined. Administration of EAME was able to reduce the increased bone turnover markers in the ovariectomised mice. Histomorphometric analysis revealed an increase in bone trabeculation and restoration of trabecular separation by EAME treatment. Metaphyseal DNA content of the femur of the OVX mice was increased by EAME administration. EAME also showed a potent antiosteoclastogenic behaviour. Thus, the present study reveals that EAME was able to successfully reduce the estrogen deficiency induced bone loss.

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Turbo methanol extract inhibits bone resorption through regulation of T cell function

Cited by 6 publications

References 39 publications

Hexane Fraction of Turbo brunneus Inhibits Intermediates of RANK-RANKL Signaling Pathway and Prevent Ovariectomy Induced Bone Loss

Hexane Fraction of Turbo brunneus Inhibits Intermediates of RANK-RANKL Signaling Pathway and Prevent Ovariectomy Induced Bone Loss

Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract ofHovenia dulcis) on the Ovariectomized Mice

Methanol Extract ofEuchelus asperPrevents Bone Resorption in Ovariectomised Mice Model

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