1997
DOI: 10.1099/0022-1317-78-3-505
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Two novel subgenomic RNAs derived from RNA 3 of tomato aspermy cucumovirus.

Abstract: Two abundant subgenomic RNAs, designated RNA 3B and RNA 5, were found to be associated with the V strain of tomato aspermy cucumovirus (V-TAV). Sequence determination showed that the 3h-terminal 323 nucleotides (nt) of RNA 3B was identical to RNA 5, whereas its 5h-terminal 163 nt was a direct repeat (one nt difference) of the 5h-half of RNA 5, and that both RNAs are completely homologous to the 3h-terminal untranslated region of TAV RNA 3. TAV RNAs 3B and 5 were also detected in the infection of a pseudorecomb… Show more

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Cited by 28 publications
(20 citation statements)
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“…This hotspot is exactly at the 59-end of the conserved motif Box-1 (Blanchard et al, 1996;Thompson et al, 2008), which is common to all subgroup II CMV strains and to TAV but is absent in subgroup I CMV strains (Palukaitis et al, 1992;Shi et al, 1997), and plays a key role in the production of subgenomic RNA5 (de Wispelaere & Rao, 2009;Thompson et al, 2008). Moreover, it has been reported as a hotspot for viral recombination between CMV RNA3 and TAV RNAs 1 and 2 (Suzuki et al, 2003) or TAV RNA3 (de Wispelaere et al, 2005), between TAV RNA3 and CMV RNAs 1 and 2 (Shi et al, 2007), and in Q-CMV (subgroup II) intragenomic recombinants (de Wispelaere & Rao, 2009).…”
Section: Discussion Effects Of 6 Nt Deletions In I17f-cmv Rna3mentioning
confidence: 99%
“…This hotspot is exactly at the 59-end of the conserved motif Box-1 (Blanchard et al, 1996;Thompson et al, 2008), which is common to all subgroup II CMV strains and to TAV but is absent in subgroup I CMV strains (Palukaitis et al, 1992;Shi et al, 1997), and plays a key role in the production of subgenomic RNA5 (de Wispelaere & Rao, 2009;Thompson et al, 2008). Moreover, it has been reported as a hotspot for viral recombination between CMV RNA3 and TAV RNAs 1 and 2 (Suzuki et al, 2003) or TAV RNA3 (de Wispelaere et al, 2005), between TAV RNA3 and CMV RNAs 1 and 2 (Shi et al, 2007), and in Q-CMV (subgroup II) intragenomic recombinants (de Wispelaere & Rao, 2009).…”
Section: Discussion Effects Of 6 Nt Deletions In I17f-cmv Rna3mentioning
confidence: 99%
“…It is interesting to consider whether the TLSs of other viruses are involved in capsid formation. Significantly, short subgenomic RNAs about 300 nt long from the 3Ј ends of CMV and tomato aspermy virus RNAs are very efficiently copackaged into virions with genomic RNAs (33,34), perhaps reflecting a TLS involvement in packaging in this sister genus to the bromoviruses.…”
Section: Discussionmentioning
confidence: 99%
“…The probe used to detect the genomic and subgenomic RNAs of TAV was a 32 Plabeled transcript complementary to nucleotides 2287-2386 of RNA 3 of V-TAV [6]. Direct RNA sequencing was performed with primers BJ20, BJ28 and BJ22 (Table 1) according to Fichot and Girard [7].…”
Section: Plant Inoculation and Progeny Viral Rna Analysesmentioning
confidence: 99%
“…Total RNAs were extracted from each infected plant at two weeks after inoculation and were analyzed by Northern blot hybridization as described [6]. The probe used to detect the genomic and subgenomic RNAs of TAV was a 32 Plabeled transcript complementary to nucleotides 2287-2386 of RNA 3 of V-TAV [6].…”
Section: Plant Inoculation and Progeny Viral Rna Analysesmentioning
confidence: 99%