Two-photon-induced fluorescence of biological markers based on optical fibers

Lago, A.; Obeidat, Amjad T.; Kaplan, A. E.; Khurgin, Jacob B.; Shkolnikov, P. L.; Stern, Michael D.

doi:10.1364/ol.20.002054

Cited by 20 publications

(11 citation statements)

References 5 publications

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“…Recently, sensors either using photo-induced electron transfer (PET) [133] to supply proton-induced "switching off of fluorescence or working by two-photon-induced fluorescence (TPIF) [267] to avoid photodamage by UV excitation in biological molecules have been developed.…”

Section: Time-resolved Fluorescencementioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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Section: Time-resolved Fluorescencementioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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“…Step-index single-mode fiber delivery had been well characterized (Bird and Gu, 2002; Helmchen and others, 2002), but their performances with step-index multimode fiber were partially described (Lago and others, 1995). The use of pulse compressor to compensate for chromatic dispersion of these step-index single-mode fibers has been studied (Helmchen and others, 2002).…”

Section: Introductionmentioning

confidence: 99%

Ultrafast optical pulse delivery with fibers for nonlinear microscopy

Kim

Choi

Yazdanfar

et al. 2008

Microscopy Res & Technique

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Nonlinear microscopies including multiphoton excitation fluorescence microscopy and multipleharmonic generation microscopy have recently gained popularity for cellular and tissue imaging. The optimization of these imaging methods for minimally invasive use will require optical fibers to conduct light into tight space where free space delivery is difficult. The delivery of high peak power laser pulses with optical fibers is limited by dispersion resulting from nonlinear refractive index responses. In this paper, we characterize a variety of commonly used optical fibers in terms of how they affect pulse profile and imaging performance of nonlinear microscopy; the following parameters are quantified: spectral bandwidth and temporal pulse width, two-photon excitation efficiency, and optical resolution. A theoretical explanation for the measured performance of these is also provided.

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“…[1][2][3] It also permits the system to be operated in novel imaging modes such as confocal microscopy, [1][2][3][4] differential interference microscopy, 5 interference microscopy, 6,7 double-pass microscopy, 8 single-photon fluorescence microscopy, 9 and two-photon fluorescence microscopy. [10][11][12] Among these, two-photon fluorescence microscopy based on an optical fiber coupler 12 is a promising tool for endoscopic imaging because of the feature of deep penetration associated with two-photon excitation 13 and the compactness of the system. In addition, a two-photon fluorescence microscope based on a fiber coupler exhibits higher axial resolution than microscopes using a largearea detector.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional optical-transfer-function analysis of fiber-optical two-photon fluorescence microscopy

Bird

2003

J. Opt. Soc. Am. A

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The three-dimensional optical transfer function is derived for analyzing the imaging performance in fiber-optical two-photon fluorescence microscopy. Two types of fiber-optical geometry are considered: The first involves a single-mode fiber for delivering a laser beam for illumination, and the second is based on the use of a single-mode fiber coupler for both illumination delivery and signal collection. It is found that in the former case the transverse and axial cutoff spatial frequencies of the three-dimensional optical transfer function are the same as those in conventional two-photon fluorescence microscopy without the use of a pinhole.However, the transverse and axial cutoff spatial frequencies in the latter case are 1.7 times as large as those in the former case. Accordingly, this feature leads to an enhanced optical sectioning effect when a fiber coupler is used, which is consistent with our recent experimental observation.

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Two-photon-induced fluorescence of biological markers based on optical fibers

Cited by 20 publications

References 5 publications

Opto-Chemical and Opto-Immuno Sensors

Opto-Chemical and Opto-Immuno Sensors

Ultrafast optical pulse delivery with fibers for nonlinear microscopy

Three-dimensional optical-transfer-function analysis of fiber-optical two-photon fluorescence microscopy

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