Type VI collagen is associated with microfibrils and oxytalan fibers in the extracellular matrix of periodontium, mesenterium and periosteum

Abstract

Type VI collagen was immunolocalized in several soft connective tissues at the light and electron microscopic level. Positive labeling was found in all tissues examined, periodontal ligament, gingiva, mesenterium and periosteum. The labeled structures could be divided into 2 categories: microfibrils intermingling with collagen fibrils, and those that formed bundles (oxytalan fibres and elastin‐associated microfibrils). Control sections incubated with antibody pre‐absorbed to purified type VI collagen, or with non‐immune antibody, proved to be negative. Our observations indicate that the structural organization of type VI collagen varies from small microfibrillar structures associated with the collagen and elastin fibre systems to highly ordered parallel arrays of oxytalan bundles.

Development of the Tarsometatarsal Skeleton by the Lateral Fusion of Three Cylindrical Periosteal Bones in the Chick Embryo (Gallus gallus)

“…Fibrillin is a component of extracellular microfibrils present characteristically in the periosteal fibrous layer (Everts et al, 1998;Yamazaki et al, 2007a), and this layer surrounds an inner osteogenic layer of the periosteum (Caplan and Pechak, 1987;Bruder and Caplan, 1989). Our results of fibrillin immunohistochemistry, therefore, represented a progressive back-to-back fusion of two fibrous layers and subsequent regression of the merged layer between adjacent bone cylinders.…”

“…In this study focusing on embryonic development of the chick TMT skeleton, we have examined morphological details of its longitudinal and radial growth as well as the process of lateral fusion, by using (1) whole mount specimens for bone and cartilage staining and micro CT analysis, (2) isolated bones for measurement and scanning electron microscopy (SEM), and (3) tissue sections for histology and immunofluorescence staining of fibrillin to delineate periosteum; fibrillin is a component of microfibrils rich in the developing periosteum (Everts et al, 1998;Yamazaki et al, 2007a). Temporospatial changes observed in bone morphology and its microstructures indicate that a rapid growth of the TMT skeleton are closely related to bone vascularization and that a lateral fusion to generate a compound TMT bone is a chronologically late event in the embryonic TMT development.…”

Type II and VI collagen in nasal and articular cartilage and the effect of IL-1α on the distribution of these collagens

“…Type II collagen is essential for the structural integrity of the tissue, whereas the other collagens are likely to play a role in cellular attachment, fibril formation and/or the three dimensional organization of cartilage (Billinghurst et al 1997). Of these collagens, type VI collagen is of considerable interest since it is assumed to function as an anchoring meshwork that connects cells to their surrounding environment (Everts et al 1998; Kuo et al 1997). …”

Efeitos do envelhecimento nos componentes fibroelásticos da junção vésico-uretral de ratos wistar.

“…Alguns trabalhos inferem possíveis funções das fibras oxitalânicas como ancoragem, manutenção da elasticidade, guia para migração celular, estabilização de vasos sanguíneos e regularização do fluxo sanguíneo (BEERTSEN et al, 1974;EVERTS et al, 1998;FULLMER et al, 1974;SAKAI et al, 1986;SIMS et al, 1973SIMS et al, , 1975TAKAGI et al, 1989).…”

“…A análise deste estudo, representado pelas figuras de 10 a 33, demonstra que as fibras elásticas maduras são formadas por componente material amorfo eletrodenso, compacto e abundante (elastina), as fibras elaunínicas por escasso material amorfo (elastina) e componente microtubular em maior quantidade e as oxitalânicas compostas apenas por componente microtubular formando feixes e dispostos paralelamente não estando associados a elastina. (GAWLIK, 1965;GOLDFISCHE et al, 1983), pele (COTTA-PEREIRA et al, 1975), periodonto (FULLMER e LILIE, 1958FULLMER, 1978;SAWADAculean et al, 2006, SCULEAN et al, 1997, ducto deferente (PANIAGUA et al, 1983), cápsula esplênica (SACHETTI, 1996), musculatura lisa (CARVALHO et al, 1997), coração (SOUZA et al, 1996), junção vésico-uretral (AWAD et al, 1976DASS et al, 1999;LÁPIDES, 1958), uretra (BUMP et al, 1988), próstata (KARLINSKI et al, 1976, fáscia transversal iguinal (RODRIGUES, Jr. et al, 1990), pulmão (NIEWOEHNER et al, 1994), colo vesical (ROTHER et al, 1996), neste estudo também confirmamos a distribuição das fibras do sistema elástico na junção vésico-uretral concordando com os achados de Lápides ( Everts et al (1998) ;Fullmer et al, (1974) Gawlik (1965Sakai et al, 1986;Sims et al (1973Sims et al ( , 1975Takagi et al (1989) relataram que as fibras que compõem o sistema elástico são evidenciadas, na microscopia de luz, pelos métodos de coloração hematoxilina férrica (Verhoeff), resorcinafucsina (Weigert) e resorcina-fucsina com prévia oxidação (Weigert-Oxona), corando fibras elásticas maduras, fibras elásticas maduras e elaunínicas, fibras elásticas maduras, elaunínicas e oxitalânicas, respectivamente. Em nosso trabalho, também foi possível identificar e tipificar o sistema elástico, através da microscopia de luz com o uso dessas colorações específicas, em que foi possível realizar a evidenciação das fibras elásticas maduras, elaunínicas e oxitalânicas na JVU, sendo assim observamos que essas fibras estão localizadas em toda a camada muscular da JVU não estando organizadas em feixes, cadeias ou lâminas como aquelas encontradas na pele …”