Über die Phosphatasen der Hefe. [4. Mitteilung zur Kenntnis der Phosphatasen.]

Albers, H.; Albers, E.

doi:10.1515/bchm2.1935.235.1-2.47

Cited by 12 publications

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“…These autolytic trials had suggested that acid phosphatase becomes susceptible to inactivation (possibly proteolysis) either during or immediately after release from the cell. In the preparative method of Albers and Albers (1), ethylacetate was used to pretreat cells prior to extraction of acid phosphatase with malt enzymes. Ethylacetate inactivates the autolytic system of yeast (4).…”

Section: Resultsmentioning

confidence: 99%

“…Ethylacetate inactivates the autolytic system of yeast (4). Several ethylacetate treatments were performed and included the pro- portions and temperature used by Albers and Albers (1) in an attempt to prepare cell ghosts rich in both acid phosphatase and fB-fructofuranosidase. The rationale was that this material could then be used with a partially purified wall-degrading enzyme system.…”

Section: Resultsmentioning

confidence: 99%

“…Details are given in the text. Symbols: 0, ethylacetate-treated cells; A, reduced cells; 0, ethylacetate plus toluene treatment according to Albers and Albers (1). The dashed line is the predicted curve for 'co-release of the two enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…The dashed line is the predicted curve for 'co-release of the two enzymes. [1] in 0.1 M sodium acetate buffer [pH 5.0]) was subjected to one or two passes through the French pressure cell at 20,000 psi. One-half of each preparation was subsequently mixed with Triton X-100 to a 0.1% final concentration.…”

Section: Discussionmentioning

confidence: 99%

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Location of Acid Phosphatase and β-Fructofuranosidase Within Yeast Cell Envelopes

Arnold

1972

J Bacteriol

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After 16 hr of incubation in a low-phosphate, aerated medium, bakers' yeast was obtained with a high titer of acid phosphatase (EC 3.1.3.2) and ,B-fructofuranosidase (EC 3.2.1.26). All of the #-fructofuranosidase and 75% of the acid phosphatase were easily released by mechanical disruption in a French pressure cell. The cell wall suffered a limited number of cracks, but this was sufficient for the co-release of these enzymes. Both enzymes were subject to autolytic release, although correlation was inconclusive because of the relative instability of acid phosphatase. The data are consistent with the bulk of the two enzymes being located in the periplasmic space. Ethylacetate treatments yielded ghosts with high ,B-fructofuranosidase but low acid phosphatase activities. The surviving acid phosphatase was not representative of that in live cells. It was resistant to release by mechanical disruption and showed a high susceptibility to heat inactivation. The p3-fructofuranosidase in live cells and in ethylacetatetreated cells exhibited polydispersity in heat inactivation susceptibility; but the kinetics were indistinguishable, and facile release by mechanical disruption was shown in both cases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Location of Acid Phosphatase and β-Fructofuranosidase Within Yeast Cell Envelopes

Arnold

1972

J Bacteriol

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Über die zusammengesetzte Natur der Fermente, I. Mitteil. (zugleich VI. Mitteil. zur Kenntnis der Phosphatasen)

Albers

Beyer

Bohnenkamp

et al. 1938

Ber. dtsch. Chem. Ges. A/B

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d, l‐α‐Tocopherol‐phosphorsäure‐ester

Karrer

Bussmann

1940

Helvetica Chimica Acta

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Auch Versuche, 3,4-Dehydroa-tocopherol durch Erhitzen von 1,5 Teilen Trimethyl-hydrochinon, 2,9 Teilen 3,7,11,15-Tetramethylhexadecin-(1)-01-(3) (Formel I) und 0,5 Teilen Zinkchlorid im Bombenrohr auf 175O herzustellen, befriedigten in der Ausbeute nicht, wenn auch das Allophanat des 3,4-Dehydro-or-tocopherols dabei in geringer Menge erhalten wurde.Von den zahlreichen, fruher hergestellten Estern des d, I-a-Tocopherolsl) hatten die meisten sehr gute Vitamin-E-Wirkung gezeigt. Aus verschiedenen Grunden schien uns auch der Phosphorsaure-ester des d, Z-a-Tocopherols von Interesse zu sein. Er lasst sich ohne Schwierigkeiten durch Einwirkuag von Phosphoroxychlorid auf das in Pyridin geloste d, I-a-Tocopherol herstellen. Sein in Wasser leicht losliches Natriumsalz bildet schaumende, seifenahnliche Losungen.Benierkenswert ist die schwere Verseifbarkeit des d, I-or-Toeopherol-phosphorsaure-esters, die wohl auf die Besetzung der beiden ortho-Stellungen der veresterten Hydroxylgruppe durch Methylseitenketten zuruckzufuhren ist. Durch Koehen mit n. alkoholischwassriger Natronlauge wurde der Ester in y2 Stunde nur zu lo%, in 1 Stunde zu 19 yo, in 2 Stunden zu ea. 40 Yo verseift. Es war daher uberrasehend, dass der d, Z-a-Tocopherol-phosphorsaure-ester bzw. sein Natriumsalz ungefahr gleich starke, bei parenteraler Verabreichung sogar bessere V i b a m i n -E -W i r k u n g wie d , Z-a-Tocop h e r o 1 s el b s t b e s i t z t . Die biologische Prufung geschah einerseits bei Hrn. Prof. H . v. EuZer (Stockholm), andererseits im pharmakologisehen Laboratorium von 3'. Hoffmmn-La Roche & Co., A.G. (Basel).Wir danken aueh an dieser Stelle fur die Ausfuhrung der Tierversuche bes tens.Fur die nachstliegende Annahme, dass der d, I-a-Tocopherolphosphorsaure-ester im tierischen Organismus durch Phosphatasen verseift wird, konnte bisher keine Stiitze gefunden werden. Wir haben ihn, bzw. sein Natriumsalz, in vitro der Einwirkung von Nieren-phosphatase2), Serumphosphatase3) und Hefeph~sphatase~) ausgel j V . Demole, 0. Isler, B. H . Ringier, H . Salornon, P. Karrer, Helv. 22, 65 (1938). z, Dargestellt nach E.

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Über die Phosphatasen der Hefe. [4. Mitteilung zur Kenntnis der Phosphatasen.]

Cited by 12 publications

References 0 publications

Location of Acid Phosphatase and β-Fructofuranosidase Within Yeast Cell Envelopes

Location of Acid Phosphatase and β-Fructofuranosidase Within Yeast Cell Envelopes

Über die zusammengesetzte Natur der Fermente, I. Mitteil. (zugleich VI. Mitteil. zur Kenntnis der Phosphatasen)

d, l‐α‐Tocopherol‐phosphorsäure‐ester

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