Uricase from Bacillus fastidious loaded in alkaline enzymosomes: Enhanced biochemical and pharmacological characteristics in hypouricemic rats

Tan, Qunyou; Zhang, Jingqing; Wang, Na; Li, Xiaoling; Xiong, Hua; Teng, Yuee; He, Dongyi; Wu, Jianyong; Zhao, Chen; Yin, Huafeng; Zhang, Liangke

doi:10.1016/j.ejpb.2012.06.002

Cited by 30 publications

(36 citation statements)

References 34 publications

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“…The experimental rat model of hyperuricemia was developed using uricase inhibitor potassium oxonate and hypoxanthine (Hou et al, 2012;Tan et al, 2012aTan et al, , 2012b. Hyperuricemia was induced by giving intraperitoneal injection of potassium oxonate (200 mg/ kg) in 0.5% carboxylmethylcellulose aqueous solution and oral administration of hypoxanthine (500 mg/kg) in aqueous solution 1 h prior to the drug administration.…”

Section: Chemically-induced Hyperuricemic Animal Modelmentioning

confidence: 99%

Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Cheng

Murugaiyah

Chan

2015

Journal of Ethnopharmacology

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Section: Chemically-induced Hyperuricemic Animal Modelmentioning

confidence: 99%

Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Cheng

Murugaiyah

Chan

2015

Journal of Ethnopharmacology

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“…For example, the catalytic activity of the artificial metalloenzyme was about eight times higher than that of MSN‐Pt/uricase at pH 2.0. The rapid loss of uricase activity in the MSN‐Pt/uricase under the acid condition was due to the free uricase with alkaline optimal pH . Further study demonstrated that with the help of the silica layer, the artificial metalloenzyme even held the strong resistance ability toward the digestion of protease.…”

Section: Resultsmentioning

confidence: 96%

“…Having demonstrated the feasibility of the artificial metalloenzyme in cells, we further carried out in vivo studies. The establishments of hyperuricemia models based on mouse and rat have grown increasingly sophisticated . Here, we chose Kunming mouse to construct the hyperuricemia model.…”

Section: Resultsmentioning

confidence: 99%

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Artificial Metalloenzyme‐Based Enzyme Replacement Therapy for the Treatment of Hyperuricemia

Liu

Zhang

et al. 2016

Adv Funct Materials

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Enzyme replacement therapy (ERT) holds great potential for treating enzyme deficiency related diseases. However, the enzymic vulnerability and the undesirable toxic side effects have strongly limited its clinical applications. Here a novel artificial metalloenzyme‐based ERT with significant self‐protection and toxic self‐cleaning ability is developed for the treatment of hyperuricemia. The uricase and platinum nanoparticles (PtNPs) are closely confined in the pores of mesoporous silica nanoparticles and encapsulated within a silica defense layer to form a tandem catalytic system. This artificial metalloenzyme keeps a high uric acid (UA) degradation activity, as well as a strong resistance capability toward various insults, such as pH, high temperature, and protease. Besides, the H2O2 produced by UA can be simultaneously eliminated by the PtNPs, resulting in significant improvement on the mammalian cell viability. In vivo studies display prominent therapy effect and no obvious side effects during the treatment of hyperuricemia mice using this artificial metalloenzyme. These results provide new insights into development of efficient and friendly enzyme replacement therapy.

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“…The main objectives of the study were to preserve maximum enzyme density at cell environment and to quantitatively enhance the enzyme without its aggregation by undergoing additional conformational changes, which determines the enzymes efficacy and selectivity [68].…”

Section: Fig 3: Specificity Of Immuno-enzymosomes To Target Cellsmentioning

confidence: 99%

Enzymosomes: A Rising Effectual Tool for Targeted Drug Delivery System

et al. 2017

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The study aims to develop enzymosomes as an emerging novel drug delivery system for site-specific action. Enzymosomes utilises the specific nature of an enzyme, which is binding to a specific substrate at a controlled rate and catalysing product production step. An enzyme is encapsulated by coupling covalently to the surface of liposomes/lipid vesicles to form enzymosomes. Enzymes links through acylation, direct conjugation, physical adsorption, encapsulation methods to prepare enzymosomes with targeted action. Such novel drug delivery systems prove effective drug release and concomitantly reduces undesirable side effects of conventional treatment methods and hence showcase improvement in the long-term therapy of the disease. They are a promising substitute to conventional treatment therapies of gout, antiplatelet activities etc. Enzymosomes are newly designed supramolecular vesicular delivery systems to be useful as a tool in pharmaceutics for the raising of drug targeting and physicochemical properties and hence bioavailability. It shows beneficial effects of drugs with a narrow precision because targeting of these drugs to their site of action improves the drugs overall pharmacodynamics and pharmacokinetic profile. It also minimizes alterations in the normal enzymatic activity, thus enhancing half-life and achieve enzyme activity on targeted sites such as cancerous cells.

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Uricase from Bacillus fastidious loaded in alkaline enzymosomes: Enhanced biochemical and pharmacological characteristics in hypouricemic rats

Cited by 30 publications

References 34 publications

Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Artificial Metalloenzyme‐Based Enzyme Replacement Therapy for the Treatment of Hyperuricemia

Enzymosomes: A Rising Effectual Tool for Targeted Drug Delivery System

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