Utilization Of Nano Ethanolic Extract Combination Chamber Bitter (Phyllanthus Niruri L.) And Garlic (Allium Sativum L.) As A Natural Immunomodulator In Nanoherbal Development, In Silico And In Vitro Study

“…The ability of lectin compounds to regulate the immune system by enhancing macrophage phagocytosis has been demonstrated [30]. Toll-Like Receptors (TLR-1 and TLR-2) bind to lectin molecules, responding to the body's innate viral immunity.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine

Adnyana

Sudaryati²

2022

Trends Sci

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The antioxidant content of Baper tea was determined using phytochemical testing, and the potential of these contents as immunomodulatory agents and candidates for standardized herbal medications was investigated. A randomized design was used in this experimental study. Phytochemical assays are used to determine the antioxidant content of Baper tea, whereas organoleptic studies are performed using hedonic tests. A literature review was used to assess the content's potential as an immunomodulatory agent and candidate for standardized herbal medicine. The study lasted four months at the Biology and National Food and Drug Agency Laboratory in Bali Province. Data analysis is based on descriptive methods. Baper tea is prepared in a 2.1.1 ratio. According to the hedonic test results, 41 panelists (87.23 %) prefer Baper tea products based on their color assessment, flavor, aroma, community reception, and acceptability. The matrix and security test revealed a water content of 4.43 %, an IC50 of 24.27 ppm, α-glucosidase inhibitors of 12.18 ppm, a total phenolic content of 28.00 %, and a total flavonoid content of 15.57 %. Baper tea raw materials contain components that have the potential to act as immunomodulatory agents and candidates for standardized herbal medications based on local wisdom. HIGHLIGHTS There are very few standardized herbal medicinal products that are efficacious as immunomodulators in the market Shallot skin (Allium Cepa), morel berry leaves (Physalis angulata), and small gooseberry leaves (Phyllanthus urinaria) packaged in Baper Tea preparations contain bioactive compounds that have the potential to be used as immunomodulatory agents and candidate for standardized herbal medicine Baper tea contains very active antioxidant compounds at a formulation ratio of 2:1:1. With panelists' acceptance of Baper Tea reaching 87.23 % GRAPHICAL ABSTRACT

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“…Penelitian lanjutan baik menggunakan uji in siliko maupun uji in vitro diperlukan untuk pengembangan agen imunomodulator. Uji in siliko Molecular Docking perlu dilakukan diantaranya untuk memastikan adanya interaksi kandungan aktif dalam ekstrak tanaman dengan protein reseptor toll like receptor (TLR) (Darmawan et al, 2017).…”

Section: Konsentrasiunclassified

Aktivitas Imunomodulator Ekstrak Metanol dan Fraksi buah Talok (Muntingia calabura L.) pada Sel RAW 264.7

Sujono

Kusumowati

Munawaroh

2021

J. Pharm. Sci. Clin. Res.

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Buah talok (Muntingia calabura L) secara empiris telah diketahui mempunyai banyak manfaat bagi kesehatan, diantaranya adalah sebagai imunomodulator. Namun mekanisme aksinya sebagai imunomodulator belum diketahui secara pasti. Penelitian ini bertujuan untuk mengevaluasi efek imunomodulator ekstrak metanolik dan fraksi buah talok pada sel makrofag RAW 264.7. Sel RAW 264.7 diberi perlakuan dengan ekstrak metanolik buah talok (EMBT), fraksi heksan buah talok (FHBT), fraksi diklorometana buah talok (FDBT), fraksi etil asetat buah talok (FEABT) serta kelompok kontrol sel (KS), kontrol lipopolisakarida (LPS) dan deksametason. Viabilitas sel diukur dengan metode MTT assay dilanjutkan isolasi RNA, pembuatan cDNA dan PCR. Level ekspresi mRNA dari gen-gen seperti toll like receptor 4 (TLR4), interferon (IFN)-ɣ, interleukin (IL)-6, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-α pada sel RAW 264.7 yang diinduksi lipopolisakarida (LPS) dianalisis dengan metode reverse transcription-PCR. Hasil penelitian menunjukkan bahwa deksametason menghambat ekspresi iNOS, IL-6, TNF-α, TLR4, IFN- ɣ dan NF-kB dibandingkan dengan kontrol sel dan LPS. Hal ini mengindikasikan bahwa deksametason mempunyai aktivitas antiinflamasi. Ekstrak metanolik (EMBT) dan fraksi buah talok (FHBT, FDBT dan FEABT) menurunkan ekspresi gen iNOS, TNF-α, IL-6, IFN- ɣ dan NF-kB jika dibandingkan dengan kontrol sel dan LPS, hal ini menunjukkan adanya aktivitas antiinflamasi. Ekstrak metanolik dan fraksi buah talok mempunyai aktivitas imunomodulator melalui penghambatan inflamasi dengan menurunkan ekspresi gen iNOS, TNF-α, IL-6, IFN- ɣ dan NF-kB pada sel RAW 264.7 yang diinduksi LPS.

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Utilization Of Nano Ethanolic Extract Combination Chamber Bitter (Phyllanthus Niruri L.) And Garlic (Allium Sativum L.) As A Natural Immunomodulator In Nanoherbal Development, In Silico And In Vitro Study

Cited by 3 publications

References 1 publication

Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application

Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application

Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine

Aktivitas Imunomodulator Ekstrak Metanol dan Fraksi buah Talok (Muntingia calabura L.) pada Sel RAW 264.7

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