UVA irradiation stimulates the synthesis of various matrix‐metalloproteinases (MMPs) in cultured human fibroblasts

Abstract

UVA irradiation leads to photoaging including clinical features such as wrinkle formation, reduced recoil capacity and blister formation of the skin. Besides synthesis of the extracellular matrix, its regulated degradation by various matrix-metalloproteinases (MMPs) determines the amount and the composition of the extracellular matrix within the dermis and the basement membrane of the dermo-epidermal junction. In this study we therefore ascertained whether UV irradiation could modulate the synthesis of MMPs with substrate specificities for dermal (collagen I, III, V) and basement membrane compounds (collagen IV, VII, proteoglycans, laminin) and whether synthesis of the counteracting tissue inhibitor of metalloproteinases (TIMP-1) was also affected. Following UVA irradiation specific mRNAs of MMPs 1, 2 and 3 were induced concomitantly up to 5-fold compared to mock irradiated controls. In contrast, TIMP-1 mRNA levels remained unaltered. Immunoprecipitation indicated that after UVA irradiation synthesis and secretion of MMPs 1, 2 and 3 into the supernatant increased. Taken together, our data show that UVA irradiation coordinately induced MMPs 1, 2 and 3 implying similar mechanisms in their regulatory pathways, while TIMP-1 synthesis was not altered. Hence, unbalanced synthesis of MMPs potentially contributes to the dissolution of dermal and basement membrane compounds finally leading to blister formation and cutaneous photoaging.

Anti-inflammatory activities of light emitting diode irradiation on collagen-induced arthritis in mice (a secondary publication)

“…UV light is known to produce free oxygen radicals at the surface of the epidermal keratinocytes, promoting the production of cytokines such as TNF-a, IL-1ß and IL-6. These cytokines cause damage to dermal cells and extracellular matrix fibers 37,38) . In the present study visible light LEDs at 570 nm and near infra-red light LEDs at 940 nm were used.…”

Nobiletin Suppresses MMP-9 Expression through Modulation of p38 MAPK Activity in Human Dermal Fibrobalsts

“…Among them, gelatinase A (MMP-2) and gelatinase B (MMP-9) were increased by UV radiation in human dermal fibroblasts (hDFs) 8,9) and are responsible for degrading collagens, which are components of the epidermal basement membranes.…”

Synthesis and Protective Effects of Bis{4-[<i>N</i>,<i>N</i>-di-(carboxymethyl)amino]phenoxy}alkane Derivatives on UVA-Induced Production of MMP-1 in Human Skin Fibroblasts

“…5,6) Inhibition of MMP-1 activation and expression has been shown to be important to alleviate UV-induced photo-damage to skin cells. 7,19,20) Compounds 4a-e showed significant protection of Cell viability is summarized from Fig.…”

“…4) Increased MMP activity and reduced collagen I expression have been considered a cause of skin aging and are observed in naturally aged and photoaged skin. [4][5][6] Therefore, inhibition of MMP production may prove effective for the treatment of photoaged skin. 7) Reactive oxygen species (ROS) is considered to be the main contributor to the deleterious effects of UVA-induced skin damage.…”