Vaccination of broiler chickens with dispersed dry powder vaccines as an alternative for liquid spray and aerosol vaccination

Corbanie, E.A.; Vervaet, Chris; Eck, J. H. H. van; Remon, Jean Paul; Landman, W. J. M.

doi:10.1016/j.vaccine.2008.06.055

Cited by 22 publications

(21 citation statements)

References 17 publications

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“…This set-up was used in a previous experiment to aerosolize NDV (Corbanie et al, 2008) and the spray-head was used previously to aerosolize fluorescent microspheres (Corbanie et al, 2006). It was found that using this set-up the concentration of NDV in the isolator did not decrease when measured up until 20 min after nebulization (Corbanie et al, 2008) and fluorescent microspheres were present throughout the respiratory tract, including air sacs, at 20 min after administration (Corbanie et al, 2006). Furthermore, in a previous experiment we performed aerosol inoculation with live H9N2 AIV (Reemers et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Animals were vaccinated in groups in isolators (Beyer & Eggelaar) with a volume of 1.38 m 3 (1.94 m long, 0.75 m wide and 0.95 m high). The vaccine was aerosolized using a Walther Pilot I spray-head with 0.5 mm nozzle (Walther Spritz-und Lackiersysteme), as described previously (Corbanie et al, 2008). After aerosolizing the vaccine, chickens were left for 1 h in the isolator to inhale the vaccine.…”

Section: Experimental Set-upmentioning

confidence: 99%

See 1 more Smart Citation

A lack of antibody formation against inactivated influenza virus after aerosol vaccination in presence or absence of adjuvantia

Geus

Haarlem

Poetri

et al. 2011

Veterinary Immunology and Immunopathology

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a b s t r a c tIn the poultry industry, infections with avian influenza virus (AIV) can result in significant economic losses. The risk and the size of an outbreak might be restricted by vaccination of poultry. A vaccine that would be used for rapid intervention during an outbreak should be safe to use, highly effective after a single administration and be suitable for mass application. A vaccine that could be applied by spray or aerosol would be suitable for mass application, but respiratory applied inactivated influenza is poorly immunogenic and needs to be adjuvanted. We chose aluminum OH, chitosan, cholera toxin B subunit (CT-B), and Stimune as adjuvant for an aerosolized vaccine with inactivated H9N2. Each adjuvant was tested in two doses. None of the adjuvanted vaccines induced AIV-specific antibodies after single vaccination, measured 1 and 3 weeks after vaccination by aerosol, in contrast to the intramuscularly applied vaccine. The aerosolized vaccine did enter the chickens' respiratory tract as CT-B-specific serum antibodies were detected after 1 week in chickens vaccinated with the CT-B-adjuvanted vaccine. Chickens showed no adverse effects after the aerosol vaccination based on weight gain and clinical signs. The failure to detect AIV-specific antibodies might be due to the concentration of the inactivated virus.

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Section: Resultsmentioning

confidence: 99%

Section: Experimental Set-upmentioning

confidence: 99%

A lack of antibody formation against inactivated influenza virus after aerosol vaccination in presence or absence of adjuvantia

Geus

Haarlem

Poetri

et al. 2011

Veterinary Immunology and Immunopathology

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“…Other vaccination methods should be investigated, e.g., the dry aerosolization of vaccine virus (5,6).…”

Section: Discussionmentioning

confidence: 99%

Effects of Temperature, Relative Humidity, Absolute Humidity, and Evaporation Potential on Survival of Airborne Gumboro Vaccine Virus

Zhao

Aarnink

Dijkman

et al. 2012

Appl Environ Microbiol

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Survival of airborne virus influences the extent of disease transmission via air. How environmental factors affect viral survival is not fully understood. We investigated the survival of a vaccine strain of Gumboro virus which was aerosolized at three temperatures (10°C, 20°C, and 30°C) and two relative humidities (RHs) (40% and 70%). The response of viral survival to four metrics (temperature, RH, absolute humidity [AH], and evaporation potential [EP]) was examined. The results show a biphasic viral survival at 10°C and 20°C, i.e., a rapid initial inactivation in a short period (2.3 min) during and after aerosolization, followed by a slow secondary inactivation during a 20-min period after aerosolization. The initial decays of aerosolized virus at 10°C (1.68 to 3.03 ln % min ؊1 ) and 20°C (3.05 to 3.62 ln % min ؊1 ) were significantly lower than those at 30°C (5.67 to 5.96 ln % min ؊1 ). The secondary decays at 10°C (0.03 to 0.09 ln % min ؊1 ) tended to be higher than those at 20°C (؊0.01 to 0.01 ln % min ؊1 ). The initial viral survival responded to temperature and RH and potentially to EP; the secondary viral survival responded to temperature and potentially to RH. In both phases, survival of the virus was not significantly affected by AH. These findings suggest that longdistance transmission of airborne virus is more likely to occur at 20°C than at 10°C or 30°C and that current Gumboro vaccination by wet aerosolization in poultry industry is not very effective due to the fast initial decay.

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“…From the start of the aerosol generation until 30 min after ending, the isolator ventilation was switched off. During aerosolization the relative humidity of the isolator air raised to 100% within a few minutes (Corbanie et al, 2008). Immediately and 10 min after ending the aerosol generation, air sampling was performed as described (Landman et al, 2004) in order to calculate the bacterial concentrations per m 3 of isolator air and subsequently the dose uptake of the birds by inhalation.…”

Section: Methodsmentioning

confidence: 99%

Reproduction of theEscherichia coliperitonitis syndrome in laying hens

Landman¹,

Heuvelink²,

Eck³

2013

Avian Pathology

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In five experiments, each consisting of four or six groups with seven or 14 brown laying hens per group, birds were inoculated with an Escherichia coli strain, isolated from a layer with the E. coli peritonitis syndrome (EPS) by different routes between 23 and 33 weeks of age. Aerosol-exposed hens inhaled 10 5.1 to 10 6.2 colony-forming units per hen; hens inoculated by other routes received 10 7.6 to 10 9.1 colony-forming units per hen. In one experiment, one-half of the birds of each group were injected intraperitoneally with sterile egg yolk simultaneously with E. coli. Dead and surviving birds were necropsied and bacteriological examination of the bone marrow was performed. The percentage of birds with EPS that died was 89 (159/179). Nearly all dead birds showed septicaemia (155/159 097%), while most had septicaemia and peritonitis (126/159 079%). Surviving hens with EPS (20/179 011%) showed chronic peritonitis and inactive ovaries. Taking all experiments together, exposure of hens by the intravenous, intratracheal and intraperitoneal routes induced EPS in 84% (41/49), 80% (55/69) and 76% (16/21), respectively, while aerosol and intravaginal exposure resulted in EPS percentages of 57% (32/56) and 49% (28/57), respectively. Except for orally inoculated groups (7/56 013% EPS), in all other groups the EPS rates differed significantly (P B0.01) from those of the placebo-exposed groups (0/42). Neither the age of hens nor the presence of free yolk in the abdomen influenced the EPS rate. The results of the present study are suggestive of the respiratory and vaginal origin of EPS in the field.

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Vaccination of broiler chickens with dispersed dry powder vaccines as an alternative for liquid spray and aerosol vaccination

Cited by 22 publications

References 17 publications

A lack of antibody formation against inactivated influenza virus after aerosol vaccination in presence or absence of adjuvantia

A lack of antibody formation against inactivated influenza virus after aerosol vaccination in presence or absence of adjuvantia

Effects of Temperature, Relative Humidity, Absolute Humidity, and Evaporation Potential on Survival of Airborne Gumboro Vaccine Virus

Reproduction of theEscherichia coliperitonitis syndrome in laying hens

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