Vacuum-Ultraviolet Circular Dichroism of Amino Acids as Revealed by Synchrotron Radiation Spectrophotometer

Matsuo, Koichi; Matsushima, Yosuke; Fukuyama, Takayuki; Senba, S.; Gekko, Kunihiko

doi:10.1246/cl.2002.826

Cited by 41 publications

(49 citation statements)

References 8 publications

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“…Figure 7 shows the VUVCD spectra of some nonpolar and polar amino acids measured over wavelengths from 260 to 140 nm at high concentrations of 2.010.0 w/v%. 24 D-and L-Isomers of alanine and proline exhibited symmetrically inverted spectra with positive and negative ellipticities, respectively, satisfying the theoretical expectations for optical isomers. The spectra of L-alanine, L-valine, L-isoleucine, and L-leucine in Figure 7a show two successive positive peaks around 200 and 180 nm.…”

Section: Structural Analysis Of Amino Acidssupporting

confidence: 76%

“…24 These high-energy electronic transitions are influenced by the solvent conditions around chromophores, which makes the VUVCD spectra useful for characterizing the hydration structures of amino acids. We measured the VUVCD spectra of several amino acids 24 and theoretically analyzed the solution structure (hydration effects) using a density functional theory (DFT) and a time-dependent DFT (TDDFT) method. 25 4.1 VUVCD Spectra of Amino Acids.…”

Section: Structural Analysis Of Amino Acidsmentioning

confidence: 99%

“…The VUVCD spectra of most L-amino acids exhibit two successive positive peaks around 200 and 180 nm, whose intensities and wavelengths are dependent on the types of side chains. 24 The theoretical reconstruction of these spectra would be useful not only for assigning the CD peaks but also for the solutionstructure analysis of amino acids, including the hydration state. As a typical example, the VUVCD spectrum of L-alanine was estimated using the DFT and TDDFT.…”

Section: Structural Analysis Of Amino Acidsmentioning

confidence: 99%

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Construction of a Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectrophotometer and Its Application to the Structural Analysis of Biomolecules

Matsuo

Gekko

2013

Bulletin of the Chemical Society of Japan

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A vacuum-ultraviolet (VUV) circular-dichroism (CD) spectrophotometer usable down to 140 nm was constructed using synchrotron radiation and applied to the structural analyses of various saccharides, amino acids, and proteins in aqueous solutions. Most monosaccharides and disaccharides exhibited a positive CD peak around 170 nm, depending on anomeric and axial/equatorial configurations of hydroxy groups, transgauche configurations of hydroxymethyl groups, and the type of glycosidic linkage. The VUVCD spectra of glycosaminoglycans sensitively reflected the characteristic contributions of constituent functional groups in the VUV region. L-Isomers of amino acids showed two successive positive peaks around 200 and 180 nm, depending on the types of side chains. The VUVCD spectrum of alanine theoretically calculated using a time-dependent density functional theory revealed the important role of hydration for stabilizing the alanine structure. The VUVCD spectra of native proteins down to 160 nm improved the predictive accuracy for the contents, numbers of segments, and sequences of ¡-helices and ¢-strands. These secondary-structure analyses were also successfully applied to various types of nonnative proteins. The obtained results demonstrate that synchrotronradiation VUVCD spectroscopy is a powerful technique for the structural analysis of biomolecules in aqueous solution, and hence could open a new field in structural biology.

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Section: Structural Analysis Of Amino Acidssupporting

confidence: 76%

Section: Structural Analysis Of Amino Acidsmentioning

confidence: 99%

Section: Structural Analysis Of Amino Acidsmentioning

confidence: 99%

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Construction of a Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectrophotometer and Its Application to the Structural Analysis of Biomolecules

Matsuo

Gekko

2013

Bulletin of the Chemical Society of Japan

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“…With this cell, we can measure the VUVCD spectra of biomaterials in the wavelength region down to 140 nm based on the higher energy transition of chromophores which cannot be obtained by a commercial CD spectropolarimeter. 10 This cell is available not only for VUVCD spectroscopy, but also for vacuum-ultraviolet absorption measurements. Simultaneous measurements of CD and UV spectra in the VUV region should open a new field in structural biology.…”

Section: Performance Of the Temperature-control Unitmentioning

confidence: 99%

Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer

et al. 2003

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“…isoleucine < leucine [65,68,69,70,71]. In this spectral range, |g → |e 1 and |g → |e 2 are the dominant transitions in CD spectra.…”

Section: Additional Considerations 54mentioning

confidence: 90%

Sum-Frequency Generation from Chiral Media and Interfaces

2006

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Professor Yuen-Ron Shen, Co-chair Professor Richard J. Saykally, Co-chair Sum frequency generation (SFG), a second-order nonlinear optical process, is electric-dipole forbidden in systems with inversion symmetry. As a result, it has been used to study chiral media and interfaces, systems intrinsically lacking inversion symmetry.This thesis describes recent progresses in the applications of and new insights into SFG from chiral media and interfaces.SFG from solutions of chiral amino acids is investigated, and a theoretical model explaining the origin and the strength of the chiral signal in electronic-resonance SFG spectroscopy is discussed. An interference scheme that allows us to distinguish enantiomers by measuring both the magnitude and the phase of the chiral SFG response is described, as well as a chiral SFG microscope producing chirality-sensitive images with sub-micron 2 resolution. Exploiting atomic and molecular parity nonconservation, the SFG process is also used to solve the Ozma problems.Sum frequency vibrational spectroscopy is used to obtain the adsorption behavior of leucine molecules at air-water interfaces. With poly(tetrafluoroethylene) as a model system, we extend the application of this surface-sensitive vibrational spectroscopy to fluorinecontaining polymers.

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Vacuum-Ultraviolet Circular Dichroism of Amino Acids as Revealed by Synchrotron Radiation Spectrophotometer

Cited by 41 publications

References 8 publications

Construction of a Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectrophotometer and Its Application to the Structural Analysis of Biomolecules

Construction of a Synchrotron-Radiation Vacuum-Ultraviolet Circular-Dichroism Spectrophotometer and Its Application to the Structural Analysis of Biomolecules

Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer

Sum-Frequency Generation from Chiral Media and Interfaces

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