Validation of a new experimental model of extrusive luxation on maxillary molars of rats: a histological study

Costa, Lidiane Cristina; Cantanhede, Luana Martins; Pereira, Erika Martins; Crivelini, Marcelo Macedo; Cuoghi, Osmar Aparecido; Pereira, Alex Luiz Pozzobon; Mendonça, Marcos Rogério de

doi:10.1007/s00784-017-2288-7

Cited by 4 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The same author, when studying the forces in nontraumatized teeth, also found no difference in root resorption data. In contrast, Costa (15) was only able to quantify root resorption differently when he induced tooth movement only 3 days after trauma.…”

Section: -Discussionmentioning

confidence: 92%

“…In this study, a device was used to perform the trauma that enabled the standardization of the magnitude and angle of force application, being these positive and differential characteristics of this method, which generated similar responses throughout the sample (15). This method of extrusive luxation resulted in a displacement of the dental element in the occlusal-mesial direction, allowing one to assess the effects of the association between the extrusive luxation and the subsequent induced tooth movement.…”

Section: -Discussionmentioning

confidence: 99%

“…In this study, a force magnitude of 50cN was employed using a NiTi closed spring, within the range recommended by the specialized literature. (15,23).…”

Section: -Discussionmentioning

confidence: 99%

“…The legs were trapped to restrict movement, the head still, and the mouth remained open during the procedures. Then, the trauma followed the methodology described by Costa et al (15) 2.3 -Induced tooth movement (ITM) Fifteen days after the EL, the ITM was started using a device according to the Heller & Nanda method (1979) (16) modi ed by replacing the steel spring with nickel-titanium and inserting a light-curing resin (Z100, 3M, St. Paul, MN, USA) in the cervical region of the incisor to improve wire retention. This procedure enabled the activation and deactivation of the spring, as well as the protection of the animal's mucosa.…”

Section: -Application Of Extrusive Luxationmentioning

confidence: 99%

“…This region was chosen because it allowed full visualization of the mesial-vestibular and intermediate roots of the right maxillary rst molar. The slides were identi ed and coded in order to establish the blinding of the examiner (14,15).…”

Section: -Scanning Of Histological Sectionsmentioning

confidence: 99%

See 4 more Smart Citations

Effects of Forces Used in Tooth Movement on the Periodontium of Teeth with Extrusive Luxation - Experimental Study in Rats

Carvalho

Amorim

et al. 2023

Preprint

View full text Add to dashboard Cite

Objectives This study assessed the effect of different types of orthodontic forces (continuous (C), continuous interrupted (Ci) and intermittent (I)) on the amount of tooth movement (TM) and root resorption (RR) mesio-vestibular and intermediate (RRmv, RRi, respectively) of teeth submitted (L) or not (nL) to extrusive luxation (EL). Data on hyaline areas were also assessed. Materials and Methods Forty-eight Wistar rats were randomly divided into 8 groups (n = 6) according to the combination of independent variables (the type of force and luxation): the moved [nL-C, nL-Ci, nL-I, L-C, L-Ci, L-I] and the non-moving [L-nM and nL-nM (control)]. The EL of the first right upper molar was performed under a force of 1500 cN for 10 seconds. After 15 days, TM was initiated with a force of 50 cN, with the three types of force (C, Ci, I). On the 14th day, the amount of TM was measured, and the animals were euthanized, the parts processed, included, cut at the cervical level of the molars, and submitted to staining with hematoxylin and eosin (HE). Results The results showed more significant movement for the C and Ci forces when compared to I in the groups submitted to luxation (p < 0.05). The luxation factor did not influence tooth movement, except for the Ci force (nL-Ci = 0.25 x L-Ci = 0.44) (p < 0.05). The amount of RR was not influenced by the type of force nor by the EL. Regarding the number of hyaline areas, it was not possible to visualize differences between the groups, under the conditions of this study. Conclusions The amount of tooth movement in teeth submitted to extrusive luxation was influenced by the type of force. In turn, the amount of root resorption was not influenced by the type of force or by the extrusive luxation. And in these induced experimental conditions were not sufficient to generate alterations in the hyaline areas. Clinical Relevance: The history of dental trauma and the type of force used during orthodontic treatment can influence the amount of tooth movement.

show abstract

Section: -Discussionmentioning

confidence: 92%

Section: -Discussionmentioning

confidence: 99%

“…In this study, a force magnitude of 50cN was employed using a NiTi closed spring, within the range recommended by the specialized literature. (15,23).…”

Section: -Discussionmentioning

confidence: 99%

Section: -Application Of Extrusive Luxationmentioning

confidence: 99%

Section: -Scanning Of Histological Sectionsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Forces Used in Tooth Movement on the Periodontium of Teeth with Extrusive Luxation - Experimental Study in Rats

Carvalho

Amorim

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Avaliação dos efeitos da castração associada ao traumatismo dentário na movimentação ortodôntica em ratos / Evaluation of the effects of castration associated with dental trauma on orthodontic movement in rats

Mezzaroba¹,

Rossi²,

Piana³

et al. 2022

BJDV

View full text Add to dashboard Cite

O sucesso da movimentação dentária em dentes traumatizados depende da severidade da injuria, sendo a reabsorção radicular uma consequência indesejável e possível de ocorrer antes, durante e após o tratamento ortodôntico. Além disso o status hormonal do paciente tem um grande impacto na conduta do profissional, uma vez que pode influenciar diretamente o sucesso do tratamento ortodôntico. O objetivo deste estudo foi avaliar os efeitos da movimentação dentária induzida e da deficiência de testosterona sobre a taxa de movimentação dentária e estrutura histológica de dentes traumatizados. Foram utilizados 24 ratos machos Wistar, separados em 4 grupos experimentais (n=6 animais/grupo): MDI: composto por animais que foram submetidos a movimentação dentária induzida (MDI); MDI+CAST: composto por animais que foram submetidos à MDI e orquidectomia bilateral; MDI+TD: composto por animais que foram submetidos à MDI e ao traumatismo dentário e MDI+CAST+TD: composto por animais que foram submetidos à MDI, ao TD e a orquidectomia bilateral. Após o período experimental os animais foram eutanasiados, pesados e as maxilas foram retiradas, fixadas em formol a 10%, descalcificadas, cortadas a 5 µm e coradas com hematoxilina e eosina. Ao final do período experimental não foram observadas diferenças estatisticamente significativas no ganho de peso corporal dos animais. A orquidectomia aumentou a taxa de MDI nos animais dos grupos MDI+CAST e MDI+CAST+TD, quando comparado aos demais grupos experimentais, sendo que a maior taxa de movimentação foi observada nos animais do grupo MDI+CAST+TD. Histologicamente, em todos os animais submetidos a movimentação ortodôntica foi observada a presença de células inflamatórias no ligamento periodontal e osteoclastos ao redor do osso alveolar. Em todos os grupos foram experimentais foram observadas lacunas de reabsorção radicular. Nos animais controle (MDI) as reabsorções foram leves afetando apenas a estrutura do cemento. Nos grupos MDI+CAST e MDI+TD foram observadas reabsorções radiculares moderadas, com degradação do cemento e dentina. No grupo MDI+CAST+TD foram observada reabsorções radiculares mais severas com extensas lacunas e degradação de dentina radicular. Conclui-se que a o déficit androgênico, induzido neste estudo pela castração dos animais, aumentou a taxa de movimentação dentária ortodôntica e a associação do traumatismo dentário com a orquidectomia potencializou os efeitos da movimentação dentária induzida sobre o processo de reabsorção radicular.

show abstract

Physiological and histological studies of dental response of Luxate tooth with application of VEGF

Al-khafaji

Faris²,

Al-Hijazi³

2022

ijhs

View full text Add to dashboard Cite

Background During luxate injury many dental tissue will be involved including pulp, periodontal ligament ,mucosa, hard dental tissue and alveolar bone with affecting the nerve and dental blood supplement. Growth factors may involved in healing process after replantation of tooth in its socket and the Vascular endothelial factor (VEGF ) is a potent one that increase angiogenesis and accelerate repairing and healing of dental pulp and the supporting tissues. The study was designed to illustrate the effect of application of growth factor on healing process of luxate molar tooth of rat. Materials & Methods Twenty male Wistar rats with luxate lower right first molar teeth were included in this study. The control were injected with normal saline (0.5μl ) around the apex of mesial root .Experimental group injected with VEGF (0.5μl ). Histological assessment of physiological responses of (dental pulp, cementum, periodontal ligament (PDL) and alveolar bone in both control and experimental groups with Receptor activator of nuclear factor kappa-Β ligand (RANKL) expression evaluation were recorded during the healing period in the examined tissue.

show abstract

Validation of a new experimental model of extrusive luxation on maxillary molars of rats: a histological study

Abstract: The validation of a new experimental method can produce more reliable evidence in further research.

Cited by 4 publications

References 20 publications

Effects of Forces Used in Tooth Movement on the Periodontium of Teeth with Extrusive Luxation - Experimental Study in Rats

Effects of Forces Used in Tooth Movement on the Periodontium of Teeth with Extrusive Luxation - Experimental Study in Rats

Avaliação dos efeitos da castração associada ao traumatismo dentário na movimentação ortodôntica em ratos / Evaluation of the effects of castration associated with dental trauma on orthodontic movement in rats

Physiological and histological studies of dental response of Luxate tooth with application of VEGF

Contact Info

Product

Resources

About