Vascular Endothelial Growth Factor-Capturing Aligned Electrospun Polycaprolactone/Gelatin Nanofibers Promote Patellar Ligament Regeneration

Yuan, Zhengchao; Sheng, Dandan; Shafiq, Muhammad; Jiang, Liping; Khan, Atta Ur Rehman; Hashim, Rashida; Chen, Yujie; Li, Baojie; Xie, Xianrui; Morsi, Yosry; Mo, Xiumei; Chen, Shiyi

doi:10.1016/j.actbio.2021.11.040

Cited by 57 publications

(31 citation statements)

References 56 publications

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“…An ideal biomaterial possesses essential properties such as hierarchical architectures and ECM composition similar to native tissues [ 41 ]. Nanofibers have been widely used in regenerative engineering due to their feasibility in modification, more interactions with tissue environment as well as delivery of regenerative signals to the tissues [ 42 ]. Among multiple modifications, cell membrane modified nanofibers exhibit more advantages such as strong hydrophilicity, high velocity of degradation and fruitfulness of bona fide surface molecules anchoring on living cells [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Immuno-activated mesenchymal stem cell living electrospun nanofibers for promoting diabetic wound repair

et al. 2022

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Diabetic wound is the leading cause of non-traumatic amputations in which oxidative stress and chronic inflammation are main factors affecting wound healing. Although mesenchymal stem cells (MSCs) as living materials can promote skin regeneration, they are still vulnerable to oxidative stress which limits their clinical applications. Herein, we have prepared (polylactic-co-glycolic acid) (PLGA) nanofibers electrospun with LPS/IFN-γ activated macrophage cell membrane. After defining physicochemical properties of the nanofibers modified by LPS/IFN-γ activated mouse RAW264.7 cell derived membrane (RCM-fibers), we demonstrated that the RCM-fibers improved BMMSC proliferation and keratinocyte migration upon oxidative stress in vitro. Moreover, bone marrow derived MSCs (BMMSCs)-loaded RCM-fibers (RCM-fiber-BMMSCs) accelerated wound closure accompanied by rapid re-epithelialization, collagen remodeling, antioxidant stress and angiogenesis in experimental diabetic wound healing in vivo. Transcriptome analysis revealed the upregulation of genes related to wound healing in BMMSCs when co-cultured with the RCM-fibers. Enhanced healing capacity of RCM-fiber-BMMSCs living material was partially mediated through CD200-CD200R interaction. Similarly, LPS/IFN-γ activated THP-1 cell membrane coated nanofibers (TCM-fibers) exhibited similar improvement of human BMMSCs (hBMMSCs) on diabetic wound healing in vivo. Our results thus demonstrate that LPS/IFN-γ activated macrophage cell membrane-modified nanofibers can in situ immunostimulate the biofunctions of BMMSCs, making this novel living material promising in wound repair of human diabetes. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Immuno-activated mesenchymal stem cell living electrospun nanofibers for promoting diabetic wound repair

et al. 2022

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“…Thus, during the past half a century, on the one hand, more and more types of materials (including natural polymers, synthesis polymers, phospholipid, surfactants, and even inorganic materials) have been tried as new drug carriers for adjusting the drug release performances [ 14 , 15 , 16 , 17 , 18 ]. Among them, polymeric excipients are the main stream [ 19 , 20 , 21 , 22 ]. On the other hand, new materials processing and treatment methods, particularly nanotechnologies, are continuously explored for encapsulating drugs into pharmaceutical excipients for increasing their therapeutic effects [ 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

et al. 2022

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One of the most important trends in developments in electrospinning is to combine itself with traditional materials production and transformation methods to take advantage of the unique properties of nanofibers. In this research, the single-fluid blending electrospinning process was combined with the casting film method to fabricate a medicated double-layer hybrid to provide a dual-phase drug controlled release profile, with ibuprofen (IBU) as a common model of a poorly water-soluble drug and ethyl cellulose (EC) and polyvinylpyrrolidone (PVP) K60 as the polymeric excipients. Electrospun medicated IBU-PVP nanofibers (F7), casting IBU-EC films (F8) and the double-layer hybrid films (DHFs, F9) with one layer of electrospun nanofibers containing IBU and PVP and the other layer of casting films containing IBU, EC and PVP, were prepared successfully. The SEM assessments demonstrated that F7 were in linear morphologies without beads or spindles, F8 were solid films, and F9 were composed of one porous fibrous layer and one solid layer. XRD and FTIR results verified that both EC and PVP were compatible with IBU. In vitro dissolution tests indicated that F7 were able to provide a pulsatile IBU release, F8 offered a typical drug sustained release, whereas F9 were able to exhibit a dual-phase controlled release with 40.3 ± 5.1% in the first phase for a pulsatile manner and the residues were released in an extended manner in the second phase. The DHFs from a combination of electrospinning and the casting method pave a new way for developing novel functional materials.

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“…It has drawn increasing attention recently because active ingredients (including those for detecting glucose) can be easily encapsulated into the polymeric nanofibers to form a new kind of functional nanocomposites ( Wang M. et al, 2021 ; Zhang M. et al, 2022 ; Zhou Y. et al, 2022 ). Based on this strategy, electrospun nanofibers have found potential applications in almost all types of scientific fields, such as energy, environment, medicine, tissue engineering, food, and agriculture ( Wang Y. et al, 2022 ; Zhang et al, 2022d ; El-Shanshory et al, 2022 ; Guo et al, 2022 ; Song et al, 2022 ; Yuan et al, 2022 ). Its popularity has a close relationship with its capability to create nanofibers, and also because this is a nano era ( Yu, 2021 ; Yu and Lv, 2022 ).…”

Section: Electrospun Glucose Sensorsmentioning

confidence: 99%

Electrospun nanofiber-based glucose sensors for glucose detection

Zhang

Liu

et al. 2022

Front. Chem.

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Diabetes is a chronic, systemic metabolic disease that leads to multiple complications, even death. Meanwhile, the number of people with diabetes worldwide is increasing year by year. Sensors play an important role in the development of biomedical devices. The development of efficient, stable, and inexpensive glucose sensors for the continuous monitoring of blood glucose levels has received widespread attention because they can provide reliable data for diabetes prevention and diagnosis. Electrospun nanofibers are new kinds of functional nanocomposites that show incredible capabilities for high-level biosensing. This article reviews glucose sensors based on electrospun nanofibers. The principles of the glucose sensor, the types of glucose measurement, and the glucose detection methods are briefly discussed. The principle of electrospinning and its applications and advantages in glucose sensors are then introduced. This article provides a comprehensive summary of the applications and advantages of polymers and nanomaterials in electrospun nanofiber-based glucose sensors. The relevant applications and comparisons of enzymatic and non-enzymatic nanofiber-based glucose sensors are discussed in detail. The main advantages and disadvantages of glucose sensors based on electrospun nanofibers are evaluated, and some solutions are proposed. Finally, potential commercial development and improved methods for glucose sensors based on electrospinning nanofibers are discussed.

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Vascular Endothelial Growth Factor-Capturing Aligned Electrospun Polycaprolactone/Gelatin Nanofibers Promote Patellar Ligament Regeneration

Cited by 57 publications

References 56 publications

Immuno-activated mesenchymal stem cell living electrospun nanofibers for promoting diabetic wound repair

Immuno-activated mesenchymal stem cell living electrospun nanofibers for promoting diabetic wound repair

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

Electrospun nanofiber-based glucose sensors for glucose detection

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