Vertical Bone Augmentation Using Bone Marrow–Derived Stem Cells

Namli, Halide; Özgür, Erdoğan; Gönlüşen, Gülfiliz; Kahraman, Onur Evren; Aydin, Halil Murat; Karabağ, Sevil; Tatlı, Ufuk

doi:10.1097/id.0000000000000334

Cited by 13 publications

(10 citation statements)

References 31 publications

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“…Recently, several studies have explored the effects of mesenchymal stem cells, which have the capability to differentiate into bone, cartilage, and muscle, on bone regeneration [67]. The use of stem cells with a synthetic or xenogenic bone graft enhanced bone regeneration when compared with a bone graft alone, possibly due to the osteogenic and osteoinductive properties of stem cells [89]. Previous studies have shown that a combination of stem cells with bone graft material improved bone regeneration [1011].…”

Section: Introductionmentioning

confidence: 99%

Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

Kang

Park

Kim

et al. 2019

J Periodontal Implant Sci

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Purpose Increased bone regeneration has been achieved through the use of stem cells in combination with graft material. However, the survival of transplanted stem cells remains a major concern. The purpose of this study was to evaluate the viability of transplanted mesenchymal stem cells (MSCs) at an early time point (24 hours) based on the type and form of the scaffold used, including type I collagen membrane and synthetic bone. Methods The stem cells were obtained from the periosteum of the otherwise healthy dental patients. Four symmetrical circular defects measuring 6 mm in diameter were made in New Zealand white rabbits using a trephine drill. The defects were grafted with 1) synthetic bone (β-tricalcium phosphate/hydroxyapatite [β-TCP/HA]) and 1×10 5 MSCs, 2) collagen membrane and 1×10 5 MSCs, 3) β-TCP/HA+collagen membrane and 1×10 5 MSCs, or 4) β-TCP/HA, a chipped collagen membrane and 1×10 5 MSCs. Cellular viability and the cell migration rate were analyzed. Results Cells were easily separated from the collagen membrane, but not from synthetic bone. The number of stem cells attached to synthetic bone in groups 1, 3, and 4 seemed to be similar. Cellular viability in group 2 was significantly higher than in the other groups ( P <0.05). The cell migration rate was highest in group 2, but this difference was not statistically significant ( P >0.05). Conclusions This study showed that stem cells can be applied when a membrane is used as a scaffold under no or minimal pressure. When space maintenance is needed, stem cells can be loaded onto synthetic bone with a chipped membrane to enhance the survival rate.

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Section: Introductionmentioning

confidence: 99%

Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

Kang

Park

Kim

et al. 2019

J Periodontal Implant Sci

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“…The surface area of the rabbit calvaria is larger compared with that of the rat and up to four experimental groups may be compared in the same animal (58,59). Compared with the rat model, the rabbit model is also advantageous because it is possible to take an equal amount of autologous bone as a control group (47). The rabbit calvaria onlay model for block bone material is easier to apply compared with the VGBR model; block bone material is placed on the calvarial bone and fixed with screws.…”

Section: Different Experimental Models Of Vertical Bone Augmentationmentioning

confidence: 99%

Animal models of vertical bone augmentation (Review)

et al. 2021

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Vertical bone augmentation is an important challenge in dental implantology. Existing vertical bone augmentation techniques, along with bone grafting materials, have achieved certain clinical progress but continue to have numerous limitations. In order to evaluate the possibility of using biomaterials to develop bone substitutes, medical devices and/or new bone grafting techniques for vertical bone augmentation, it is essential to establish clinically relevant animal models to investigate their biocompatibility, mechanical properties, applicability and safety. The present review discusses recent animal experiments related to vertical bone augmentation. In addition, surgical protocols for establishing relevant preclinical models with various animal species were reviewed. The present study aims to provide guidance for selecting experimental animal models of vertical bone augmentation. Contents 1. Introduction 2. Different experimental models of vertical bone augmentation 3. Discussion 4. Conclusion

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“…In contrast, other studies [28, 74–77] have shown that, owing to the strong hydrophobicity of this material, it does not promote cell adhesion. Surface treatments or association with biomolecules, such as collagen, alginate, or polypeptides, are therefore necessary to facilitate the interaction between the cells and the biomaterial [75]. In fact, Wang et al demonstrated that the incorporation of MSCs in nanoparticles mixed with calcium phosphate cements (CPC), inserted into cranial defects in nude rats, promoted bone regeneration [28].…”

Section: Scaffolds With Stem Cellsmentioning

confidence: 99%

“…In fact, Wang et al demonstrated that the incorporation of MSCs in nanoparticles mixed with calcium phosphate cements (CPC), inserted into cranial defects in nude rats, promoted bone regeneration [28]. Thus, these scaffolds are suitable for bone regeneration, showing good results in newly formed bone volumes in animal studies [75, 78]. …”

Section: Scaffolds With Stem Cellsmentioning

confidence: 99%

Emerging Perspectives in Scaffold for Tissue Engineering in Oral Surgery

Ceccarelli

Presta

Benedetti

et al. 2017

Stem Cells International

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Bone regeneration is currently one of the most important and challenging tissue engineering approaches in regenerative medicine. Bone regeneration is a promising approach in dentistry and is considered an ideal clinical strategy in treating diseases, injuries, and defects of the maxillofacial region. Advances in tissue engineering have resulted in the development of innovative scaffold designs, complemented by the progress made in cell-based therapies. In vitro bone regeneration can be achieved by the combination of stem cells, scaffolds, and bioactive factors. The biomimetic approach to create an ideal bone substitute provides strategies for developing combined scaffolds composed of adult stem cells with mesenchymal phenotype and different organic biomaterials (such as collagen and hyaluronic acid derivatives) or inorganic biomaterials such as manufactured polymers (polyglycolic acid (PGA), polylactic acid (PLA), and polycaprolactone). This review focuses on different biomaterials currently used in dentistry as scaffolds for bone regeneration in treating bone defects or in surgical techniques, such as sinus lift, horizontal and vertical bone grafts, or socket preservation. Our review would be of particular interest to medical and surgical researchers at the interface of cell biology, materials science, and tissue engineering, as well as industry-related manufacturers and researchers in healthcare, prosthetics, and 3D printing, too.

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Vertical Bone Augmentation Using Bone Marrow–Derived Stem Cells

Abstract: BMSCs delivered with a collagen/β-TCP linked scaffold can provide improved new bone formation that is comparable with autogenous bone block graft through vertical guided bone regeneration technique.

Cited by 13 publications

References 31 publications

Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

Animal models of vertical bone augmentation (Review)

Emerging Perspectives in Scaffold for Tissue Engineering in Oral Surgery

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