Vi Antigens of the Enterobacteriaceae. IV. Purification and Properties of Vi Antigen of S. paratyphi C.

Baker, Edgar E.; Whiteside, Roberta E.

doi:10.3181/00379727-105-26100

Cited by 10 publications

(23 citation statements)

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“…Each of these bacteria must contain very similar Vi antigens (henceforth referred to only as antigen) for they show complete cross reactivity in agglutination and quantitatively cross absorb Vi antibody (Kauffmann, 1941;Felix, 1952). More recently Webster et al (1954), Landy, Webster and Sagin (1954), and Baker et al (1959) demonstrated certain quantitative differences in purified antigen preparations from different sources, but pointed out that these variations could represent differences in state of purity, or changes introduced during purification.…”

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confidence: 99%

PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

1960

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confidence: 99%

PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

1960

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“…This is not unexpected since the extraction of Vi antigen from bacterial cells is normally done by initially shaking whole bacterial cells in 0-15 M NaCl solution (Landy & Lamb, 1953;Baker et al 1959;Wong & Feeley, 1972). We have demonstrated the presence of such released soluble Vi antigen from the supernatant of different batches of Vi bacterial cell suspensions using a precipitation reaction against a commercial anti-Vi antiserum.…”

Section: Discussionmentioning

confidence: 99%

Vi serology in screening of typhoid carriers: improved specificity by detection of Vi antibodies by counterimmunoelectrophoresis

Chau

Tsang

1982

J. Hyg.

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SUMMARYA purified soluble Vi antigen was used in counterimmunoelectrophoresis (CIE), passive haemagglutination (HA) and solid phase radio-immunoassay (SPRIA) for detection of serum Vi antibody. Serum Vi antibody was detected in 13 of 14 chronic typhoid carriers by both CIE and HA. SPRIA studies showed that Vi antibodies in sera of these carriers were mainly of the IgG class. Successful treatment with amoxycillin in one carrier, who initially showed a positive Vi test, resulted in a reversion in 6 months to seronegativity as measured by Vi CIE. However, Vi antibody was also detected in 10 sera (3 %) from 329 control subjects by HA but not by CIE. When the CIE assay was applied to 1030 serum specimens obtained from hospitalized patients, it was able to detect one typhoid carrier, but one (0 1 %) non-carrier also gave a positive Vi CIE test. It is thus concluded that Vi CIE is more specific and no less sensitive than Vi HA for the detection of the typhoid carrier state. The finding by SPRIA that the anti-Vi antibodies present in typhoid carriers' sera were mainly of the IgG class further justified the use of CIE as a precipitation test for their detection.

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“…P. ballerup was used as antigen for the preparation of Vi antiserum, and Citrobacter strain 5396/38 was used for citrobacter antiserum. The rabbits were exsanguinated by cardiac puncture 1 If not pure, repeat precipitation with hexadecyltrimethylammonium bromide and ethanol, as above, until free of somatic antigens.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Vi Antigen and a Simple Method for Its Measurement

Wong

Feeley

1972

Applied Microbiology

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Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.

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Vi Antigens of the Enterobacteriaceae. IV. Purification and Properties of Vi Antigen of S. paratyphi C.

Cited by 10 publications

References 0 publications

PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

PRODUCTION OF Vi ANTIGEN ON A CHEMICALLY DEFINED MEDIUM BY A COLIFORM BACTERIUM

Vi serology in screening of typhoid carriers: improved specificity by detection of Vi antibodies by counterimmunoelectrophoresis

Isolation of Vi Antigen and a Simple Method for Its Measurement

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