Viabilitas sel fibroblas BHK-21 pada permukaan resin akrilik rapid heat cured (Viability of fibroblast BHK-21 cells to the surface of rapid heat cured acrylic resins)

Yuliati, Anita

doi:10.20473/j.djmkg.v38.i2.p68-72

Cited by 11 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The value of absorbent from each well represents the number of cell viability in media culture. 25 Measurement of cell viability was performed using Optical Density formula as follows: 23,24 A material is categorized as non-toxic when the number of living cell or cell viability results greater than 60%.…”

Section: Methodsmentioning

confidence: 99%

Toxicological Screening of Ellagic Acid in Pomegranate Fruit and Hydroxyapatite Combination as Bone Graft Material on BHK-21 Fibroblast Cell

Wardhana¹,

Erlita²,

Nirwana³

et al. 2020

Dentino

View full text Add to dashboard Cite

Background: Bone graft is an alternative therapy for periodontitis and other bone destructive lesions. Several studies had revealed Ellagic Acid (EA) ability in increasing osteogenesis process. EA contains polyphenols, such as Ellagitannin, Gallotannin, and Anthocyanin, which demonstrate anti-inflammatory and antibacterial activity as well as growth factor stimulating effect. EA combination with bone graft material (hydroxyapatite) is anticipated to enhance bone osteogenesis yet no investigation was performed to identify its toxicity towards fibroblast cell. Objective: To analyze EA toxicity on fibroblast cell in vitro. Methods: This was a true experimental study using post-test only with control group design. Fibroblast cell was exposed with EA in eight different concentrations: 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5%. Control group comprised of cell control and media group. All groups were exposed to MTT Assay test and measured using Elisa Reader. Result: The calculation of cell viability value in EA groups at 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4% and 5% concentration were 88.2%, 92.3%, 97.5%, 89.5%, 84.2%, 90.7%, 88.9% and 89.4% respectively. Conclusion: All EA and hydroxyapatite combinations are not toxic towards BHK-21 fibroblast cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Toxicological Screening of Ellagic Acid in Pomegranate Fruit and Hydroxyapatite Combination as Bone Graft Material on BHK-21 Fibroblast Cell

Wardhana¹,

Erlita²,

Nirwana³

et al. 2020

Dentino

View full text Add to dashboard Cite

show abstract

“…The commonly used compound is MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) 6 . In this study human gingival fibroblast cells were used because fibroblast cells are the most important cells in human periodontal tissue 7 This study aimed to determine the concentration of EcengGondok leaf extract which can maintain the viability of human gingival fibroblast cells for 24 hours.…”

Section: Introductionmentioning

confidence: 99%

Viability Test of Water Hyacinth Leaf Extract (Eichornia Crassipes) on Human Gingival Fibroblast Cells

2020

MLU

View full text Add to dashboard Cite

Periodontal disease is a chronic inflammatory disease caused by colonization of bacteria that affects soft tissues and hard tissues that support the teeth, inflammation and rejuvenation of alveolar bone are signs of periodontal disease. Fibroblast also play a role in producing and maintaining extracellular matrix, cell proliferation and cell differentiation in response to prolonged tissue injury and chronic inflammation. The components of water hyacinth are very beneficial for health such as phenols, alkaloids, flavonoids, tannins. This study aimed to determine the concentration of EcengGondok leaf extract which can maintain the viability of human gingival fibroblast cells for 24 hours. The method human gingival primary cell culture was harvested, placed on a 96-well microplate. Each well on the microplate was given water hyacinth leaf extract with a concentration of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.25 mg/ml, 0.125 mg/ml, 0.0625 mg/ ml, 0.0312 mg/ml, 0.0156 mg/ml for 24 hours. MTT assay was carried out by adding MTT solution after 24 hours of incubation. Formazan optical density values are read by ELISA reader with a wavelength of 590 nm, viability is obtained by calculating the viability formula. Results are viability of human gingival fibroblast cells was good starting in the treatment group 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml and 0.0156 mg/ml. Conclusion the highest viability of human gingival fibroblast cells in the treatment group of 0.0156 mg/ml was 75.98%.

show abstract

“…In the dentistry field, bone graft materials have been used in the oral cavity, nevertheless, must be biocompatible. It means that bone graft materials can be accepted by the host, non-toxic, irritant, non-carcinogenic, and non-allergen [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture

2018

View full text Add to dashboard Cite

AimThis study aims to examine the toxicity of collagen extracted from gouramy fish scales (Oshpronemus gouramy) by evaluating its viability against baby hamster kidney fibroblasts-21.Materials and MethodsCollagen was extracted from gouramy fish scales (O. gouramy) with 6% acetic acid. Its results were analyzed using Fourier-transform infrared spectroscopy and freeze-dried technique. Its morphology then was analyzed with scanning electron microscope. Afterward, 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay was conducted to compare cells with and without fish scale collagen treatment.ResultsCollagen extracted from gouramy fish scales had no influence statistically on cultured fibroblast cells with a statistical significance (2-tailed) value of0.754 (p>00025).ConclusionCollagen extracted from gouramy fish scales has high viability against BHK21 fibroblast cells.

show abstract

Viabilitas sel fibroblas BHK-21 pada permukaan resin akrilik rapid heat cured (Viability of fibroblast BHK-21 cells to the surface of rapid heat cured acrylic resins)

Abstract: Acrylic resins are widely used in the fabrication

Cited by 11 publications

References 10 publications

Toxicological Screening of Ellagic Acid in Pomegranate Fruit and Hydroxyapatite Combination as Bone Graft Material on BHK-21 Fibroblast Cell

Toxicological Screening of Ellagic Acid in Pomegranate Fruit and Hydroxyapatite Combination as Bone Graft Material on BHK-21 Fibroblast Cell

Viability Test of Water Hyacinth Leaf Extract (Eichornia Crassipes) on Human Gingival Fibroblast Cells

Viability test of fish scale collagen (Oshpronemus gouramy) on baby hamster kidney fibroblasts-21 fibroblast cell culture

Contact Info

Product

Resources

About