Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species

Development of a Rapid, Efficient, Intelligent and Cost-Saving Tool to Diagnose Pasteurella Multocida by Using Whole Genome Sequence and Genotypes of Pasteurella Multocida From Different Hosts

“…Relying on only one or two typing systems is di cult to address the characteristics of P. multocida isolates from different host species and/or their association with different diseases. For example, P. multocida isolates from different host species might have the same capsular genotypes but possess different LPS genotypes and/or MLST genotypes; even those from different host species that share the same capsular, LPS, and MLST genotypes might carry different VFGs [27,23]. Therefore, we have proposed a combined "capsular: LPS: MLST" genotyping system that includes virulence genotyping to discriminate P. multocida isolates from different host species and/or those associated with different diseases [5].…”

“…Our PmGT platform also uses the machine-learning technology to predict the host species of P. multocida strains according to the whole genome sequence. Although many epidemiological and genomic studies have shown that P. multocida isolates from different hosts have preferences for different capsular/LPS/MLST genotypes and/or VFGs [18,19,5,27,6], not all P. multocida isolates could be typed through the capsular genotyping system and/or MLST genotyping system [19,18,5] (Supplementary Table S1), and in addition, we have determined that P. multocida strains with the same capsular: LPS: MLST genotypes can be isolated from different host species [23]. For example, capsular: LPS: MLST genotype B: L2: ST44 strains have been isolated from hemorrhagic septicemia cases in pigs (e.g.…”

“…Because P. multocida strains colonized wide spectrums of host species, we therefore intend to develop a model to predict the host tropism of emerging P. multocida strains based on their genome sequences. Many epidemiological and genomic studies on P. multocida have revealed that VFGs show a correlation with host species [5,19,27]. These ndings make it possible to determine the host tropism of a P. multocida strain by analyzing the VFGs carried by its whole genome sequence.…”

Caracterização genética e perfil de sensibilidade a antimicrobianos de isolados de >i<Pasteurella multocida>/i< oriundos da cavidade bucal de gatos domésticos sadios e com gengivite

“…A cápsula é considerada um dos principais fatores de virulência da P. multocida e foi identificada em 100% dos isolados estudados sendo identificada predominantemente como do tipo A. Vários estudos têm mostrado que esse tipo capsular é o mais frequente em isolados de P. multocida oriundos de diferentes espécies animais (ARUMUGAM et al, 2011;DAVIES;MACCORQUODALE;REILLY, 2004;EWERS et al, 2006;GARCIA et al, 2011;FURIAN et al, 2013), inclusive em gatos domésticos e em humanos UJVÁRI et al, 2019;UJVÁRI;MAKRAI;MAGYAR, 2019 MAMOREH, 2014) e de gatos . A importância das proteínas codificadas pelos genes supracitados é confirmada pela alta frequência que os mesmos são identificados em isolados de P. multocida (BETHE et al, 2009;DAVIES;MACCORQUODALE;REILLY, 2004;EWERS et al, 2006).…”

“…As cepas de P. multocida são classificadas em 16 sorotipos diferentes a partir da composição dos antígenos somáticos de LPS (HEDDLESTON; GALLAGHER; REBERS, 1972) e em oito genótipos(HARPER et al, 2015). Em gatos, já foram identificados os genótipos L1, L3 e L7 associados ao LPS (UJVARI; MAKRAI;MAGYAR, 2019). O LPS é um importante constituinte da membrana externa das bactérias Gram negativas e é formado por três componentes típicos, Estrutura da parede celular de bactérias Gram negativas Fonte: Portal eDiciplinas -USP.…”