Vitamin E induces regular structure and stability of human insulin, more intense than vitamin D3

Soleymani, Hossein; Saboury, Ali Akbar; Moosavi-Movahedi, Ali Akbar; Rahmani, Fatemeh; Maleki, Javad; Yousefinejad, Saeed; Maghami, Parvaneh

doi:10.1016/j.ijbiomac.2016.09.047

Cited by 12 publications

(11 citation statements)

References 65 publications

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“…INS fluorophore emissions were regulated in the range of 300-400 nm, with a maximum emission at 340 nm (Iannuzzi et al 2017b). The fluorescent intensity of insulin at a wavelength of about 303 nm decreased, widened, and produced a redshift (Soleymani et al 2016). This phenomenon was found in the interaction of vitamin E with human insulin.…”

Section: Resultsmentioning

confidence: 72%

“…Through the summary in Table 3, it can be observed that both of the thermodynamic parameters ΔH 0 were negative, ΔS 0 were obtained positive (− 16.514 kJ/mol and 22.65 J/mol•K). Based on three types of molecular interactions, INS and PHL intrigue can occur by hydrophobic force, hydrogen bond, and disperse forces at temperatures of 288 K, 298 K, and 308 K. ΔH 0 and ΔS 0 can affect protein denaturation (Soleymani et al 2016). Soleymani et al reported the results of the differential analysis calorimetric scanning (DSC) of human insulin.…”

Section: Thermodynamic Parameters and Interaction Force Of Phl And Insmentioning

confidence: 99%

“…The interaction of protein molecules in Thymus DNA with abiraterone was also studied using spectroscopic methods (Alsaif et al 2020a;Wani et al 2020a). Insulin has interacted with vitamin E (Soleymani et al 2016). There are three types of chromophore groups in proteins, which have different electron absorption band characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Interaction between phloretin and insulin: a spectroscopic study

Yanti

Agrawal

et al. 2021

J Anal Sci Technol

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Diabetes is among the top ten deadly diseases in the world. It occurs either when the pancreas does not produce enough insulin (INS) or when the body cannot effectively use the insulin it produces. Phloretin (PHL) has a biological effect that can treat diabetes. A spectroscopic study was carried out to explore the interaction between phloretin and insulin. UV/Vis spectroscopy, fluorescence spectroscopy, and circular dichroism spectropolarimeter were used in the study. UV/Vis spectra showed that the interaction between PHL and INS produced strong absorption at a wavelength of 282 nm. The fluorescence analysis results showed that the excitation and emission occurred at 280-nm and 305-nm wavelengths, respectively. Temperature changes did not affect INS emissions. However, the interaction of PHL–INS caused a redshift at 305 to 317 nm. Temperature affected the binding constant (Ka) and the binding site (n). Ka decreased with increasing temperature and increased the binding site. The thermodynamic parameters such as enthalpy (ΔH0) and entropy (ΔS0) each had a value of − 16,514 kJ/mol and 22.65 J/mol·K. PHL and INS interaction formed hydrogen bonds and hydrophobic interaction. The free energy (ΔG0) recorded was negative. PHL and INS interactions took place spontaneously. The quenching effect was dynamic and static. KD values were greater than KS. The higher the temperature, the less was KD and KS. The appearance of two negative signals on circular dichroism (CD) spectropolarimeter implies that phloretin could induce regional configuration changes in insulin. The addition of PHL has revealed that the proportion of α-helix in the insulin stabilizes its structure. Phloretin’s stabilization and enhancement of the α-helix structural configuration in insulin indicate that phloretin can improve insulin resistance.

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Section: Resultsmentioning

confidence: 72%

Section: Thermodynamic Parameters and Interaction Force Of Phl And Insmentioning

confidence: 99%

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Interaction between phloretin and insulin: a spectroscopic study

Yanti

Agrawal

et al. 2021

J Anal Sci Technol

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“…The role of vitamin E supplementation in the management of DKD remains to be fully clarified. While in vitro studies demonstrated metabolic effects of antioxidant supplementation, in particular regarding insulin biology and activity [9,10], clinical studies are far from well establishing the mechanisms by which supplementation can be useful. Undoubtedly, the renal protection of these compounds moves primarily from their anti-oxidant and anti-inflammatory potential.…”

Section: Diabetes Oxidative Stress and Inflammationmentioning

confidence: 99%

Antioxidant, Anti-Inflammatory, and Metabolic Properties of Tocopherols and Tocotrienols: Clinical Implications for Vitamin E Supplementation in Diabetic Kidney Disease

Vincenzo

Tana

et al. 2019

IJMS

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Diabetes mellitus is a metabolic disorder characterized by the development of vascular complications associated with high morbidity and mortality and the consequent relevant costs for the public health systems. Diabetic kidney disease is one of these complications that represent the main cause of end-stage renal disease in Western countries. Hyperglycemia, inflammation, and oxidative stress contribute to its physiopathology, and several investigations have been performed to evaluate the role of antioxidant supplementation as a complementary approach for the prevention and control of diabetes and associated disturbances. Vitamin E compounds, including different types of tocopherols and tocotrienols, have been considered as a treatment to tackle major cardiovascular outcomes in diabetic subjects, but often with conflicting or even negative results. However, their effects on diabetic nephropathy are even less clear, despite several intervention studies that showed the improvement of renal parameters after supplementation in patients with diabetic kidney disease. Then we performed a review of the literature about the role of vitamin E supplementation on diabetic nephropathy, also describing the underlying antioxidant, anti-inflammatory, and metabolic mechanisms to evaluate the possible use of tocopherols and tocotrienols in clinical practice.

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“…1 Also, the frequent monitoring of glucose is an essential part of diabetes management. 2,3 In this way, several kinds of electrochemical [4][5][6] and optical 1,7 methods were implemented for glucose monitoring. But among them, the colorimetric methods are preferred due to its simplification, convenience, continuous monitoring, practically and rapid determinations.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric nanomolar determination of glucose by using C-dots/$$\hbox {Fe}_{3}\hbox {O}_{4}$$ magnetic nanozyme

Sadravi

Honarasa

2019

J Chem Sci

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C-dots/Fe 3 O 4 magnetic nanocomposite was known as peroxidase-like nanozyme. In this work, C-dots/Fe 3 O 4 magnetic nanocomposite was used to determine glucose in the presence of glucose oxidase (GOx). The color change of 3,3 ,5,5-tetramethylbenzidine (TMB) was used as an indication of glucose in a colorimetric assay. The nanozymatic activity of C-dots/Fe 3 O 4 magnetic nanocomposite to detect glucose was depended on TMB and C-dots/Fe 3 O 4 nanozyme concentration and pH. The linear range and detection limit to determine glucose using C-dots/Fe 3 O 4 magnetic nanocomposite were obtained as 5 × 10 −8 to 1 × 10 −3 M and 50 nM, respectively. Also, the sensing system shows high selectivity towards the detection of glucose in the presence of fructose, lactose and maltose as interferences. The results indicate that this assay is simple, cheap, and highly sensitive and selective for glucose determination.

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Vitamin E induces regular structure and stability of human insulin, more intense than vitamin D3

Cited by 12 publications

References 65 publications

Interaction between phloretin and insulin: a spectroscopic study

Interaction between phloretin and insulin: a spectroscopic study

Antioxidant, Anti-Inflammatory, and Metabolic Properties of Tocopherols and Tocotrienols: Clinical Implications for Vitamin E Supplementation in Diabetic Kidney Disease

Spectrophotometric nanomolar determination of glucose by using C-dots/$$\hbox {Fe}_{3}\hbox {O}_{4}$$ magnetic nanozyme

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