Wound Healing Effect of Kaempferol in Diabetic and Nondiabetic Rats

Özay, Yusuf; Güzel, Sevda; Yumrutaş, Önder; Pehlivanoğlu, Burçin; Erdoğdu, İbrahim Halil; Yıldırım, Zühal; Türk, Bilge; Darcan, Sinan

doi:10.1016/j.jss.2018.08.009

Cited by 109 publications

(66 citation statements)

References 38 publications

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“…The tissue samples were fixed in formalin and embedded in paraffin. The the tissue sections with 5 μm thickness were taken and stained with HE [2].…”

Section: Wound Induction and Treatmentmentioning

confidence: 99%

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Antihyperglycemic and diabetic wound healing activity of smallanthus sonchifolius leaves extract

et al. 2018

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Diabetic patient tend to suffer from lower extremity complication that contribute to the occurence of diabetic foot ulcer. Smallanthus sonchifolius or yacon leaves extract has been used as an alternative medicine for diabetes and also showed antibacterial activity. This research aims to obtain the antihyperglycemic and diabetic wound healing activity of yacon leaves extract. Diabetic condition of rat was induced by streptozotocin (45 mg/kg bw) and nicotinamide (110 mg/kg bw). Rats were then allowed to develop diabetes for 21 days. A biopsy punch then was used to create a wound. Yacon leaves extract were administered by oral and topical for 14 day. The serum glucose level and diameter of the wound were measured every week, and histopatology study of the skin was conducted at the end of the study. Oral administration of yacon leaves extract (150 mg/kg bw) together with topical administration showed antihyperglycemic as well as diabetic wound healing activities comparable to the reference drugs (glibenclamide oral and nebacetin cream).

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“…The tissue samples were fixed in formalin and embedded in paraffin. The the tissue sections with 5 μm thickness were taken and stained with HE [2].…”

Section: Wound Induction and Treatmentmentioning

confidence: 99%

“…On of the key problem is high blood glucose level. High blood glucose level inhibits wound healing process associated with prolonged inflammatory phase [2].…”

Section: Introductionmentioning

confidence: 99%

Antihyperglycemic and diabetic wound healing activity of smallanthus sonchifolius leaves extract

et al. 2018

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“…In addition, methanol was found to extract many compounds rather than other solvents [37]. Flavonoids are well known for secondary metabolites that show a good significant contribution against wound-healing activities [38,39]. Kaempherol and luteolin are classified under flavonoid compounds that have been proved to exert wound-healing activities on diabetic and non-diabetic rats [38][39][40].…”

Section: Metabolite Profiling Of C Papaya Extract From Reflux Techniquementioning

confidence: 99%

Bioassay-Guided Different Extraction Techniques of Carica papaya (Linn.) Leaves on In Vitro Wound-Healing Activities

et al. 2020

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Herbal plants are traditionally utilized to treat various illnesses. They contain phytochemicals that can be extracted using conventional methods such as maceration, soxhlet, and boiling, as well as non-conventional methods including ultrasonic, microwave, and others. Carica papaya leaves have been used for the treatment of dengue, fungal, and bacterial infections as well as an ingredient in anti-aging products. Phytochemicals analysis detected the presence of kaempferol, myricetin, carpaine, pseudocarpaine, dehydrocarpaine I and II, ferulic acid, caffeic acid, chlorogenic acid, β-carotene, lycopene, and anthraquinones glycoside. Conventional preparation by boiling and simple maceration is practical, simple, and safe; however, only polar phytochemicals are extracted. The present study aims to investigate the effects of three different non-conventional extraction techniques (ultrasonic-assisted extraction, reflux, and agitation) on C. papaya phytochemical constituents, the antioxidant capacity, and wound-healing activities. Among the three techniques, the reflux technique produced the highest extraction yield (17.86%) with the presence of saponins, flavonoids, coumarins, alkaloids, and phenolic metabolites. The reflux technique also produced the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging with an IC 50 value of 0.236 mg/mL followed by ultrasonic-assisted extraction (UAE) (IC 50 : 0.377 mg/mL) and agitation (IC 50 : 0.404 mg/mL). At tested concentrations (3.125 µg/mL to 500 µg/mL), all extracts do not exhibit a cytotoxicity effect on the human skin fibroblast, HSF1184. Interestingly, reflux and UAE were active fibroblast proliferators that support 85% (12.5 µg/mL) and 41% (6.25 µg/mL) better cell growth, respectively. Additionally, during the early 24 h of the scratch assay, the migration rate at 12.5 µg/mL was faster for all extracts with 51.8% (reflux), 49.3% (agitation), and 42.5% (UAE) as compared to control (21.87%). At 48 h, proliferated cells covered 78.7% of the scratch area for reflux extract, 63.1% for UAE, 61% for agitation, and 42.6% for control. Additionally, the collagen synthesis was enhanced for 31.6% and 65% after 24 and 48 h of treatment for reflux. An HPLC-MS/MS-QTOF (quadruple time-of-flight) analysis of reflux identified nine phytochemicals, including carpaine, kaempferol 3-(2G-glucosylrutinoside), kaempferol 3-(2"-rhamnosylgalactoside), 7-rhamnoside, kaempferol 3-rhamnosyl-(1->2)-galactoside-7-rhamnoside, luteolin 7-galactosyl-(1->6)-galactoside, orientin 7-O-rhamnoside, 11-hydroperoxy-12,13-epoxy-9-octadecenoic acid, palmitic amide, and 2-hexaprenyl-6-methoxyphenol. The results suggested that reflux was the best technique as compared to ultrasonic and agitation.

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“…Kaempferol (KP), a natural compound, is widely used in antioxidative, anti-inflammatory, antiallergic, and anticancer applications [13,14]. It has been reported that KP is an effective wound-healing agent in the treatment of both nondiabetic and diabetic wounds [15,16]. However, its poor water solubility, instability, and short half-life in vivo metabolism limit the clinical applications of KP [17].…”

Section: Introductionmentioning

confidence: 99%

Modification of Alginate Hydrogel Films for Delivering Hydrophobic Kaempferol

Zhang

Deng

et al. 2019

Journal of Nanomaterials

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The high hydrophilicity of alginate materials makes delivering a high load of hydrophobic drug difficult. To overcome this drawback, sodium alginate (SA) was modified using octylamine (OA), and then kaempferol (KP) was combined with the alginate solution through the hydrophobic interaction between OA and KP. The modified SA/KP (MSA/KP) hydrogel films were ionically cross-linked by immersing in calcium chloride solution. Subsequently, the products were analyzed via Fourier transform infrared spectroscopy, scanning electron microscopy, contact angle test, thermogravimetric analysis, swelling analysis, water vapor transmission, and mechanical property test. Results showed that compared with the SA hydrogel films, the MSA/KP hydrogel films exhibited an interporous structure, good swelling, and mechanical properties. Moreover, the drug release experiments demonstrated that the MSA/KP hydrogel films achieved a KP encapsulation efficiency of 70.4%, with a sustained KP release of up to 80 h. The cell viability experiments demonstrated that the MSA/KP hydrogel films posed no evident cytotoxicity to human umbilical vein endothelial cells.

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Wound Healing Effect of Kaempferol in Diabetic and Nondiabetic Rats

Cited by 109 publications

References 38 publications

Antihyperglycemic and diabetic wound healing activity of smallanthus sonchifolius leaves extract

Antihyperglycemic and diabetic wound healing activity of smallanthus sonchifolius leaves extract

Bioassay-Guided Different Extraction Techniques of Carica papaya (Linn.) Leaves on In Vitro Wound-Healing Activities

Modification of Alginate Hydrogel Films for Delivering Hydrophobic Kaempferol

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