Zirconium–metalloporphyrin frameworks as a three-in-one platform possessing oxygen nanocage, electron media, and bonding site for electrochemiluminescence protein kinase activity assay

Zhang, Guangyao; Cai, Chang Qing; Cosnier, Serge; Zeng, Haibo; Zhang, Xueji; Shan, Dan

doi:10.1039/c6nr01206j

Cited by 71 publications

(50 citation statements)

References 60 publications

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“…sun et al localized surface plasmon resonance, 44,45 surface plasmon resonance (SPR), 46 electrochemiluminescence, [47][48][49][50] and mass spectrometry. 51,52 Among these methods, colorimetric assays based on the aggregation or redispersion of gold nanoparticles (AuNPs) are in particular prevalent because of their simple manipulation principle and easy detection procedure.…”

mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications.

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mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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“…[91] Shan et al utilized zinc metalloporphyrinic MOFs (MOF-525(Zn)) to develop an ECL biosensor for ah ighly sensitive protein kinase A( PKA) activity assay. [92] 3. 4…”

Section: Biosensing Of Biomacromoleculesmentioning

confidence: 99%

Porphyrin‐Based Metal–Organic Frameworks for Biomedical Applications

2020

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Porphyrins and porphyrin derivatives have been widely explored for various applications owing to their excellent photophysical and electrochemical properties. However, inherent shortcomings, such as instability and self‐quenching under physiological conditions, limit their biomedical applications. In recent years, metal–organic frameworks (MOFs) have received increasing attention. The construction of porphyrin‐based MOFs by introducing porphyrin molecules into MOFs or using porphyrins as organic linkers to form MOFs can combine the unique features of porphyrins and MOFs as well as overcome the limitations of porphyrins. This Review summarizes important synthesis strategies for porphyrin‐based MOFs including porphyrin@MOFs, porphyrinic MOFs, and composite porphyrinic MOFs, and highlights recent achievements and progress in the development of porphyrin‐based MOFs for biomedical applications in tumor therapy and biosensing. Finally, the challenges and prospects presented by this class of emerging materials for biomedical applications are discussed.

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“…[91] Shan et al nutzten Zink-metalloporphyrinische MOFs (MOF-525(Zn)) fürd ie Entwicklung eines ECL-Biosensors,u md ie hochempfindliche Proteinkinase-A (PKA)-Aktivitätzumessen. [92] Das DBP(Pt)-Netzwerk und RBITC haben die gleiche Aktivierungsenergie,j edoch interferieren die Phosphoreszenz und Fluoreszenz nicht miteinander,w odurch die Bestimmung des intrazellulären Sauerstoffgehalts ermçglicht wird. Die Dickdarmkrebszellen CT26 einer Maus wurden mit R-UiO-MOFs unter verschiedenen Sauerstoffdrücken (pO 2 )b ehandelt, nämlich 4mmHg (Hypoxie), 32 mmHg (Normoxie) und 160 mmHg (Sättigung), und durch Auswertung der ratiometrischen Lumineszenzbilder konnten Drücke von 5.1 AE 2.5, 27.3 AE 3.1 und 158 AE 11 mmHg berechnet werden.…”

Section: Biosensorik Von Biomakromolekülenunclassified