Infectious diseases caused by bacteria have become a public health issue. Antibiotic therapy for infectious disorders, as well as antibiotic overuse, has resulted in antibiotic-resistant bacterial strains. Zeolitic imidazolate framework-8 (ZIF-8) possesses a wide surface area, high porosity, variable functionality, and potential drug carriers. We have established a clear method for making a nanoscale APE@ZIF-8 nanocomposite agent with outstanding antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and cephalosporin-carbapenem-resistant Escherichia coli (CCREC). We present a unique approach for encapsulating molecules ofAjuga parviflora extract (APE) with ZIF-8. APE@ZIF-8 has a positive charge. By electrostatic contact with the negatively charged bacterial surface of S. aureus and E. coli, APE@ZIF-8 NPs produce reactive oxygen species (ROS) that damage bacterial cell organelles. As a result, the APE@ZIF-8 nanocomposite offers limitless application potential in the treatment of infectious disorders caused by drug-resistant gram-positive and gram-negative bacteria.

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The current study was done to evaluate the chemical composition, antioxidant and antibacterial activities of Drymaria cordata extract in three solvents i.e. methanol (DCM), hexane (DCH) and water (DCW) against chloramphenicol-resistant Bacillus subtilis and β-lactams-resistant Pseudomonas aeruginosa. Phytochemical screening and Fourier transform-infrared spectroscopy (FTIR) showed the presence of phenols, amines, hydroxy group, and amino-related components. Gas chromatography-mass spectrometry (GCMS) depicts the presence of antibacterial compounds such as hexanedioic acid, hexadecanoic acid, nonadecadiene, hexadecen-1-ol, octadecadienoic acid and neophytadiene. DPPH free radicals scavenging assay exhibit maximum percentage inhibition in DCM (71.57 %) at the concentration of 1000μg/ml. The total antioxidant through phosphomolybdate assay was observed in DCM with values 834.44 ± 4.01 mg AAE/g of extract. For antibacterial activities, DCM shows best zone of inhibition (ZOI) (8 mm at 100 µg/ml to 15.17 mm at 1000 µg/ml) and minimum inhibitory concentration (MIC) (75 µg/ml) as compared to other extracts against Bacillus subtilis. Also, DCM shows zone of inhibition (ZOI) (6.83 mm to 13.17 mm) and minimum inhibitory concentration (MIC) (70 µg/ml) as compared to other extracts against Pseudomonas aeruginosa. These results indicate that the Drymaria cordata methanol extract possesses good antibacterial and antioxidant properties which justify its use against pathogenic bacteria.

Cancer is one of the world's fastest-emerging noncommunicable diseases and one of the major causes of death. Zeolitic imidazolate framework-8 (ZIF-8) is a subclass of metal-organic frameworks made up of organic linkers and inorganic nodes. Responsive nanocarriers with biocompatibility and precise drug release ability, superior stability under physiological conditions, pH responsiveness, and tunable drug release properties have emerged as prospective properties of ZIF-8. Among the different options proposed for cancer treatment, natural products are used to find novel hits, which emerge as one of the most successful methods with no adverse effects. Here, we developed a novel nanoscale ZIF-8 core encapsulated drugs extracted from Ajuga bracteosa extract in its frameworks through a facile and efficient strategy. ZIF-8@ABE NPs show a sixfold improvement in instability compared with that of free ABE. It can internalize the NPs into cells and present targeted effects of inhibition of growth on the human Caucasian lung carcinoma cell line (A549) (adenocarcinoma). The sustained release behavior of ZIF-8@ABE has shown a higher efficacy than the free ABE. We envisage that such a biocompatible and biodegradable delivery system may promote the development and translation of hybrid superstructures into smart and personalized delivery platforms, but the limitations imposed by limited loading capacities have stymied the development of innovative multifunctional drug delivery systems.