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The effects of ozone at different concentrations (10, 30, 45 g/m 3 ) on fluidity and thermotropic properties of erythrocyte membranes were investigated by EPR using two spin probes: 5-doxylstearic acid (5-DSA) and 16-doxylstearic acid (16-DSA). The effect of ozone on the erythrocyte membrane fluidity was a dose-dependent process. The ozone at concentration of 10 g/m 3 caused rigidization of the membrane while at concentration of 45 g/m 3 increased fluidity both on the surface and in the deeper hydrocarbon region of the membrane. Temperature transitions close to the polar heads region (monitored by 5-DSA) were not sensitive to an increase in ozone concentration. In the case of 16-DSA, low temperature thermotropic transition (around 20°C) gradually decreased with the increase of ozone concentration. High temperature transition (around 40°C) significantly differed at the ozone concentration of 10 g/m 3 and 45 g/m 3 , being higher and lower, respectively, as compared to untreated cells. For the ozone concentration of 45 g/m 3 the disappearance of the low temperature break and the appearance of two breaks at 37°C and 16°C were observed. nism of therapeutic ozone action is still un-Abbreviations: 5-DSA, 5-doxylstearic acid; 16-DSA, 16-doxylstearic acid; EPR, electron paramagnetic resonance; NaCl/P i , phosphate-buffered saline.

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