Since collagen derived products are highly used in food supplements due to its bioactive properties by influencing cellular and tissue health, flexibility with high effect of repairing and adaptation. It is necessary to carry out safety requirement of an oral chronic toxicity assessment. This study investigated the adverse effect of long term oral administration of collagen-® in pregnant rabbits. We have conducted a thirty days randomized controlled trial in pregnant rabbits. Rabbits were randomized into two groups; Group 1 (control group) were administrated with 1 ml of normal saline PO, SID while Group 2 (collagen-α group) were administered with 1 ml of collagen-α ® PO, SID for one month. Bodyweight gain, viscera weight and histopathological evaluation of the individual rabbit were recorded. The results revealed that the body weight gain and visceral tissues weight in the collagen-α group were significantly lower than the control group. Moreover, various histopathological changes were recorded in heart, lungs, liver and kidneys of the collagen-α group when compared with the non-significant changes that noticed in the control group. Under the conditions of the current study, a high risk of chronic toxic effect was observed in pregnant rabbits inoculated with 1 ml of oral dose of collagen alpha supplement. This result suggests that the adverse effect of collagen derived supplements is overbalancing the benefits.

The current study was carried out to investigate the toxic effects of aflatoxin B1 exposure on heart, lung, and testis of male Albino rats. Twelve healthy male Albino rats were used in the current study. They were divided equally into 2 groups (control and treated groups). The treated group was given aflatoxin B1 orally by a gastric tube at concentration 0.5 mg per kg of body weight for 7 day. The control group was given distilled water alone. At the end of experiment (1 week), the rats were killed under ether anesthesia and the internal organs(heart, lung, and testis) were collected immediately for histological examination. The histopathology examination in heart revealed that there is oedema between myocardial. In lung, there is a large number of inflammatory cells and dilated the alveolar sac with exudates. The testis revealed the excessive vacuolated cells and suppression spermatogenesis. In conclusion, oral dose of aflatoxin B1 exposure could induce a deleterious effect on the histological structure of the heart, lung, and testis of male Albino rats. High level of attention must be taken to prevent the possible contamination of our food with aflatoxin B1.

Creatine is a substance found naturally in body tissues, especially in the muscles and brain. Creatine is found in the diet, especially in seafood and red meat, and is commonly used to increase muscle mass and improve exercise performance in humans. This research was design to evaluate the effects of creatine administration on many physiological and biochemical parameters. In the method design, 24 mature adult male rabbits are divided at random into four groups as the following: G1 (Group of control): six mature adult of male rabbits orally administered distilled H2O (1ml/animal) by gavage daily, G2 mature adult of male rabbits were orally administration creatine monohydrate (1ml/kg BW) by gavage daily. G 3 mature adult of male rabbits were orally administration creatine monohydrate (2ml/kg BW) by gavage daily. While G4 adult male rabbits were orally administration creatine monohydrate (3ml/kg BW). After 30-days of administration, the rabbits were anaesthetized and blood samples were collected for determination thyroid hormones, liver enzymes, lipid profiles and kidney function (determined by urea, creatinine levels) and serum total protein levels, in the results, the groups that received 1, 2 and 3 ml of creatinine significantly increased but more over increased in group treated with 3 ml of creatinine in all studied parameters such as T4 hormones, liver enzymes, urea, creatinine levels and all lipid profile except TSH, T3, HDL, total protein concentration decreased significantly compared to the control group. In conclusions, the creatine has harmful effect on biochemical and physiological parameters in the creatine-treated group (3ml). In the conclusion that an adverse effect of creatine on the functions of the thyroid gland, liver and kidneys increases in these organs with increase doses.