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Improvements have been made to a previously described multi-mycotoxin method that involved a membrane cleanup step. Using 2-dimensional thin layer chromatography and appropriate solvent systems, aflatoxin B1 can be detected in mixed feedstuffs and various ingredients at levels ranging from 0.1 to 0.3 μg/kg. Corresponding detection limits for ochratoxin A and sterigmatocystin are 5 to 20 μg/kg and for T-2 toxin and zearalenone 20 to 200 μg/kg.

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