Worms are still a serious problem for poor and developing countries. Children, especially school-age children, are more at risk of infection. Efforts need to be made to prevent the effects of worms. Prevention can be done through a promotive approach. This observational study with a pre-posttest and cross-sectional approach is aimed at evaluating the effectiveness of health education on healthy and helminthic behavior and analyzing the impact of helminthiasis on the deficit in the diet and children’s learning achievement. The number of samples is 60 students from five grade 3 and 4 elementary schools in North Pontianak, West Kalimantan. The sampling technique was carried out by proportional random sampling. Worm infection in elementary school students was 16.7%, anemia was 55%, and learning achievement scores were less than the average grade of 55%. There was a significant difference in health counseling towards a decrease in the worm number (p=0.046). There was a significant relationship between healthy living behavior and helminthiasis (p=0.005). There was a significant relationship between helminthiasis and anemia (p=0.017). There is a relationship between helminthiasis and learning achievement in elementary school children (p=0.017). There is a relationship between anemia and learning achievement (p=0.005). It is necessary for public health centers to provide treatment services for worms and health education about the effects of helminthiasis on health and learning achievement. The school should provide hand washing facilities in schools, and parents should play an active role in improving clean and healthy lifestyle habits at home.

Microscopic examination results in patients with filariasis are often not identified by the presence of microfilaria, so it needs to be checked by Polymerase Chain Reaction (PCR). One PCR method uses High-Resolution Melting (HRM). The purpose of this study was to compare qPCR-HRM with microscopic examination methods to determine the types of microfilaria found in patients with filariasis. 19 samples were examined using a microscopic method and qPCR-HRM. The results of microscopic examination found no type of microfilaria and in qPCR-HRM identified B.malayi and W.bancrofti with peak temperature melting 78.1-78.7 ℃ and 80.2-80.8 ℃. The results of the study based on the comparison of two methods show that the types of microfilaria W.bancrofti and B.malayi can be found using qPCR-HRM.

Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.

Lack of mineral and vitamin intake, especially from fruit and vegetable consumption at school age, can increase the risk of illness, impaired growth and development, and impaired adaptation to environmental hazards. This study aims to analyze the effect of educational media with processed noodles on the acceptance and consumption of vegetables and fruit in elementary school children. This type of research is quasi-experimental, with a pre and post design approach. Data collection techniques were carried out using a Comstock visual checklist sheet and a recall form to see vegetable and fruit consumption. The results show that using educational media with processed noodles can increase the acceptance of fruit and vegetable consumption in school-age children. The composition of nutrients in each processed vegetable and fruit noodle is quite complete and balanced.

The adhesion of Rifampicin-resistant TB to neutrophils plays an essential role in colonization. Several active compounds in papaya leaf and Miana leaf (Coleus scutellarioides L) are believed to regulate or prevent the formation of bacterial colonies. The purpose of this study was to determine the anti-bacterial effectiveness of extracts of papaya leaf (Carica Papaya L.) and Miana leaf (Coleus scutellarioides L) against bacterial isolates of Rifampicin-Resistant TB strain (RR). This research method is a in vitro laboratory experiment, and extracts of papaya leaf and Miana leaf (50, 25, 12,5%) were tested as anti-bacterial using the M-TB susceptibility test using the Proportion Method. The results showed the anti-bacterial ability of papaya leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 0% so that it was included in the Sensitive category, but at a concentration of 25% the resistance percentage value was 42.86% % and a concentration of 12.5%, the percentage value of resistance is 42.86% so that it is included in the category of resistance (Resistant > 1% and Sensitive < 1%). The anti-bacterial ability of miana leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 5.33%, at a concentration of 25%, with a resistance percentage value of 17.14%, and at a concentration of 12.5%, with a resistance percentage value of 100%, so all are included in the resistant category. The Conclusion 50% papaya leaf extract inhibits the formation of Rifampicin-resistant MTBC-resistant bacterial colonies, allowing its usage as a substitute ingredient in Rifampicin-resistant MTBC-resistant medications.