One hundred thirty one clinical bacterial samples were collected from skin, pus, urine and blood from patients at Al-Salam General Teaching Hospital in Mosul city during March until July/ 2012, The present study included identification using API ID 32 STAPH system to the species

Serological detection of IgG antibodies to antigens one soil isolate 0f thermophilic actinomycetes belonged to the species Thermoactinomyces vulgaris in (70) sera of patients with farmer's lung disease and hay fever patients compared with control normal peoples in the same environmental condition were carried out using ELISA method. The results indicated that (19) sera from symptomatic patients having IgG level between (1.007-1.626) I.U/ml, four of them having farmer's lung disease and the remaining have hay fever as diagnosed by clinical. The IgG level between (0.293-0.944) I.U/ml indicated negative and which was found in(16) patients two of them having farmer's lung disease and the remaning have hay fever. The IgG level of normal people were devided in two groups: first account positive control was between (1.089-2.147) I.U/ml and the second account negative was between (0.819-0.959) I.U/ml. The present study has demonstrated the usefulness of estimation of specific IgG antibodies activity to thermophilic actinomyces by ELISA for the diagnosis of farmer's lung disease as a screening test of large number of samples with sufficient high sensitivity.

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Microorganisms play an important role in causing diseases, sinusitis is one of these diseases which cause a health problems to many people. This study therefore was oriented to nominate the species of microorganisms isolated from Acute, Subacute and Chronic sinusitis. The causative organisms were identified by biochemical and physiological tests, and their susceptibility to selective antibiotics were tested, the Colony Forming Unit (CFU/cm 3) and the number of pus cells were also determined. The following bacterial species were isolated from sinusitis patients: Klebsiella pneumoniae (24%), Streptococcus viridans (9%), Staphylococcus aureus (8%), Bacillus spp. (6.7%), Staphylococcus epidermidis (6.1%), Streptococcus pneumoniae (4.9%), Proteus vulgaris (4%), Escherichia coli (3%), Moraxella catarrhalis (2%), Streptomyces somaliensis (1.2%), Corynebacterium diphtheriae (0.6%), Coryn. Pseudodiphtheriticum (0.6%), Coryn. Haemolyticum (0.6%), Coryn. Pyogenes (0.6%), Nocardia asteroids (0.6%), Listeria monocytogenes (0.6%), Candida albicans (0.6%). On the other hand Haemophilus influenzae could not be isolated. Chronic sinusitis dominated in male cases (11-15) years old and in female (16-20) years old. The antibiotic biogram showed that Ciprofloxacin has an activity against all bacterial isolates which were resistant to Colxacillin, Penicillin G and Ampicillin and produced β-lactamase.