Background: Platelet concentrates are extensively utilized in the medical and dental field to promote tissue regeneration. The profusion of endogenous growth factors in platelets α-granules transmit their use for enhanced wound healing. However, little attention has been given to study their antimicrobial potential. This study was conducted to assess the antibacterial and antifungal property of platelet-rich fibrin (PRF) and PRF matrix (PRFM). Materials and Methodology: Blood samples were obtained from 16 participants, PRF and PRFM were processed as per the protocol prescribed by Choukroun et al . and Lucarelli et al ., respectively. The susceptibility test against microbiota in the root canal and Candida albicans was assessed through minimum inhibition zone by agar diffusion technique. Results: PRF showed an effective antibacterial property, however, did not perform well against C. albicans strains. PRFM did not show any antibacterial or antifungal properties. Conclusions: The antibacterial efficacy of PRF may prove beneficial when used in the revascularization procedure of immature necrotic teeth.

Dens invaginatus (DI) is one of the rare malformations of teeth which results from an infolding of the dental papilla during the development of teeth. This defect gives rise to a possible communication between the pulp and oral environment, thereby increasing the susceptibility to caries, pulpitis, and pulp necrosis. Thus, early detection and conservative management of this invaginatus is of utmost importance. The present case series describes a conservative endodontic treatment technique for the management of teeth with Type II DI using a guided endodontic approach with three-dimensional printed surgical stents. This technique provides a precise and minimally invasive approach in the conservative management of DI, without compromising the vitality of main pulpal tissue.

Background: Dental anxiety is a significant and challenging problem in patients seeking dental treatment. During any dental procedure patients often experience severe pre-operative and intra-operative anxiety which makes the procedure more difficult. Anxiety during endodontic therapy often leads to increased pain perceptions and instability of the physiological parameter throughout treatment. Aim: The purpose of this study was to evaluate the influence of music as a nonpharmacologic aid on anxiety, pain perception and physiological parameters (heart rate, systolic blood pressure, and diastolic blood pressure) at three different time period - before, during, and after endodontic treatment. Methods: A total of 100 patients were recruited in the present study. Before starting the endodontic treatment, the interviewer administered the Modified Corah Dental Anxiety Scale (MCDAS) to assess the baseline level of anxiety and Visual Analog Scale (VAS) for pain assessment. Patients were randomly divided into 2 groups: the first one listened to the music during endodontic treatment and the second one did not. Before, during, and after the endodontic procedure, the physiological parameters (i.e. heart rate, systolic and diastolic blood pressure) were recorded. All the data were collected and statistically analyzed. Results: Direct contrasts between patients listening or not listening to music showed that all the measured physiological parameters improved considering the overall period (during and after the root canal therapy) in the group of patients listening to music (P < .05). Conclusion: Within the limitation of the study, music administered to subjects with different levels of anxiety during endodontic treatment significantly decreased pain levels and improved physiological parameters during the endodontic procedures. Clinical Significance: Music and medicine always work together and the soothing effects of sounds and musical frequencies make this union an extraordinary tool of synergistic care. Music therapy can be considered as a valid nonpharmacologic aid to manage anxiety during endodontic treatment.

Aim: This study aimed to compare the surface roughness of two rotary nickel–titanium instrument systems before and after clinical use using atomic force microscope (AFM). Methods: Sixteen instruments of two rotary nickel–titanium systems were used in this study. Eight MTwo (VDW, Munich, Germany) instruments and eight Neoendo flex rotary files (Orikam Healthcare, India) were evaluated. New and the used rotary files of the two experimental groups were evaluated. Instruments were analyzed on a point at a 5-mm section from the tip of the instrument. Quantitative measurements according to the topographical deviations were recorded. The data were statistically analyzed with paired t-test and independent samples t-test. Results: The mean root mean square (RMS) values for new and used MTwo 25.06 files were 1.23 ± 0.176 nm and 1.58 ± 0.284 nm, respectively, and the difference between them was statistically significant (P < 0.05). The mean RMS values for new and used NeoEndo Flex Files 25.06 were 0.96 ± 0.34 nm and 1.76 ± 0.414 nm, respectively; the difference between them also was statistically significant (P < 0.05). Conclusion: According to the AFM analysis, the instruments underwent surface deterioration after clinical use and MTwo had better surface quality than NeoEndo Flex Files.