Penelitian ini bertujuan untuk mengetahui daya hambat VCO terhadap pertumbuhan Candida albicans isolat vagina. Uji daya hambat VCO terhadap C. albicans isolat vagina dilakukan dengan metode difusi cakram. C. albicans yang diisolasi memiliki ciri-ciri koloni yeast, membentuk blastospora, pseudohifa, klamidospora, dan germ tube. VCO mampu menghambat pertumbuhan C. albicans isolat vagina. VCO pada konsentrasi 90% memiliki daya hambat tertinggi, dengan nilai zona hambat minimum 24,0 mm, lebih besar dibandingkan konsentrasi 75% (20 mm), 50% (9,7 mm), 25% (1,9 mm), dan kontrol negatif (0,0 mm). Namun nilai Zona Hambat tersebut lebih rendah secara bermakna dengan kontrol positif (Ketokenazol 2%) (p< 0,05). VCO mengandung berbagai zat aktif yang dapat bekerja sebagai anti fungi, seperti asam laurat, asam kaprilat, dan asam kaprat. Zat monolaurin dan monokaprin yang dihasilkan VCO dapat merusak membran sel jamur. Berdasarkan hasil tersebut VCO berpotensi digunakan sebagai obat alternatif untuk infeksi C. albicans Kata kunci: Daya hambat, VCO, Candida albicans, isolate vagina Abstratc The purpose of this study to determine the resistivity of VCO against the growth of vaginal isolates of Candida albicans. The VCO resistivity test against vaginal isolates of C. albicans is performed by the disc diffusion method. C. albicans was successfully isolated from the vaginal swab which is characterized by the yeast colonies, forming blastospores, pseudohifa, chlamydospora, and germ tube. Disc diffusion test results showed that VCO can inhibit the growth of C. albicans fungus. VCO at a concentration of 90% had the highest resistivity, with a minimum inhibit zone value of 24.0 mm, greater than VCO at concentrations of 75% (20 mm), 50% (9.7 mm), 25% (1.9 mm), and negative control (0.0 mm). However, the value of the Inhibitory Zone was significantly lower with positive control (Ketokenazol 2%) (p <0.05). VCO contains various active substances that can be working as anti-fungi, such as lauric acid, caprylic acid, and acid kaprat. Substances monolaurin and monokaprin which VCO produce can damage the structure of cell membrane fungus. Based on these results, the VCO is potentially used as an alternative medicine for infection of C. albicans

Averrhoa bilimbi L., popularly known as belimbing wuluh, is a common plant used as traditional medicine and cooking ingredients in Indonesia. Averrhoa bilimbi fruit contains many active substances as antibacterial such as tannin, formic acid, flavonoids, saponins, peroxides, and triterpenoids. Streptococcus pyogenes is a pathogenic bacteria that can cause respiratory infections. This study was carried out to investigate the inhibitory activity of Averrhoa bilimbi L. fruit extract at various concentrations on the growth of Streptococcus pyogenes. This study was a true experimental with post test only control design. The inhibitory activity was assessed by the KirbyBauer’s disc diffusion method with four different extract concentrations (20%, 40%, 60%, 80%), 96% EtOH as negative control, and Amoxicillin antibiotic as positif control. The EtOH extract showed the mean diameter of inhibition zone of 20% extract was 11.43 mm (strong), 40% extract was 19.13 mm (strong), 60% extract was 24.58 mm (very strong), 80% extract was 30.99 mm (very strong), and the negative control (96% EtOH) was 0.00 mm. Least Significant Difference test showed there were significant different of inhibition zone in each extract concentrations of Averrhoa bilimbi L. against Streptococcus pyogenes with the strongest inhibitory activity is 80% extract concentration. Keywords: Averrhoa bilimbi, Inhibitory activity, Streptococcus pyogenes

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.

Gonorrhea is a sexually transmitted disease caused by Neisseria gonorrhoeae. Gonorrhea infection varies greatly in the community, especially in sexually active women. Infection cases in women are often asymptomatic, that can causes complication can easily occur. The aims of this study are to identify and determine sensitivity of Neisseria gonorrhoeae bacteria against cefixime antibiotics. This study was using a descriptive method and used 30 samples. Neisseria gonorrhoeae was isolatd from the vaginal swab sample of Commercial Sex Workers at Puskesmas II Denpasar Selatan. The cultured bacteria on Thayer Martin media were then identified by gram staining, oxidase test, and catalase test. Neisseria gonorrhoeae sensitivity test against cefixime was performed by disc diffusion method. The results of identification showed that 6 (20 %) of 30 samples were Neisseria gonorrhoeae bacteria with cell characteristics in the form of diplococcus, gram-negative, oxidase and catalase positive. The results of the sensitivity test showed that all isolats of Neisseria gonorrhoeae were resistant to cefixime

Abstract. Urine is the rest of the body's metabolism that is released through the process of urinalization. Urine examination aims to determine the condition of the kidney and other urinary tracts. Delays in examinations in the laboratory often occur resulting in disturbed urine stability. This study aims to determine the effect of delays on the number of urine leukocytes in patients with urinary tract infections. This research with posttest only control group design using four control and treatment groups each consisting of 9 samples. The control group is the result of urine examination which is examined before 30 minutes while the treatment group is the result of urine examination after 1, 2 and 3 hours since being collected. The results showed an average difference between the control and treatment groups, especially there was a significant reduction in the 3-hour delay group.Keywords: urine leukocytes, delay in urine examination