ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC 50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat F E ) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3',4'-pentahidroksi flavonol.Kata kunci : Eucresta horsfieldii Lesch Benn., antioksidan, flavonol ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC 50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates F E ) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC 50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat F E ) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3',4'-pentahidroksi flavonol.Kata kunci : Eucresta horsfieldii Lesch Benn., antioksidan, flavonol ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant acti...

Background: Grapes (Vitis vinifera) are a source of antioxidants with high content of polyphenols and anthocyanins. Grape skin is rich in flavonoids. Anthocyanins and flavonoids have the effect of lowering blood triglyceride levels. Purposes: This study aimed to determine the effect of using green grape extract (GGE) in lowering triglyceride levels in the blood. Methods: Five groups of white male rats (Rattus novergicus) were divided into a negative control group (KN), positive control (KP), rats with a GGE dose of 100 mg/200g BW as P1, rats with a GGE dose of 250mg/200g BW as P2, and rats with GGE dose of 500 mg/200g BW as P3. Each group was given a hypercholesterolemic diet for five weeks. Groups P1, P2, and P3 were given GGE according to their respective doses for 14 days, then measured triglyceride levels in the blood. Results: The results showed that GGE 500mg/200g BW significantly reduced triglyceride levels in all treated mice. Conclusion: These results indicate that GGE has a great potential to treat dyslipidemia by lowering triglyceride levels in the blood.

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.

Tumbuhan Kepuh (Sterculia foetida L.) merupakan salah satu tumbuhan yang berpotensi sebagai sumber obat baru yang kaya akan senyawa  yang digunakan sebagai obat-obatan modern, intermediet farmasi dan senyawa utama dalam bahan obat alami. Pengobatan tradisional di Bali sering disebut Usada yang memuat  tentang ilmu mendiagnose penyakit, bahan-bahan obat, dan doa-doa. Masyarakat juga mengenal yang namanya ayurweda yaitu pemnfaatan obat herbal dan terapi yoga. Pengembangan pengobatan tradisional menjadi obat herbal (fitofarmaka), diperlukan analisis terhadap aktivitas senyawa metabolit sekunder yang ada pada tumbuhan Kepuh (Sterculia foetida L.). Selain itu juga perlu diketahui pengaruh pemberian terapi yoga pada pengobatan tradisional. Penelitian ini menggunakan metode deskriptif dengan menggunakan metode review artikel terstruktur. Berdasarkan data primer yang diperoleh dari beberapa jurnal ilmiah diketahui aktivitas biologis yang dimiliki metabolit sekunder yang ada pada tumbuhan Kepuh (Sterculia foetida L.) adalah  antibakteri, antioksidan, antijamur, antiprotozoal, sitotoksik, trombolitik, anti-rematik, analgesik, antipiretik, antidiabetes, insektisida, antifeedant, dan efek mikrobisida. Sehingga secara ilmiah tumbuhan Kepuh (Sterculia foetida L.) dapat digunakan sebagai sumber obat baru yang kaya akan senyawa  yang digunakan sebagai obat-obatan modern, intermediet farmasi dan senyawa utama dalam bahan obat alami. Begitupula pengaruh pemberian terapi yoga pada pengobatan tradisional yang merupakan pengobatan secara non farmakologis mampu membantu proses penyembuhan pada penderita penyakit tertentu. Akan tetapi perlu dilakukan penelitian lanjutan terhadap pemanfaatan tumbuhan Kepuh sebagai obat herbal yang dikombinasi dengan terapi yoga.

Background: Pharmacoecomics has been defined as the description and analysis of the costs of drug therapy to health care systems and society. Pharmacoeconomics research identifies, measure, and compares the costs and consequences of pharmaceutical product and service. Within this framework, research and methods related to cost-minimization, cost-effectiveness, quality-of-life and other humanistic assessments are include. In-essence, pharmacoeconomics analysis uses tools for examining the impact of alternative drug therapies and other medical interventions Based on this background, the author is interested to explain about the role of Pharmacoecomics on health service in pharmacist students at Bintang Persada School, Denpasar. Purpose: The purpose of this activity is as a form of implementation of Tri Dharma Stikes Bali Wisnu Dharma Denpasar. Methods: Participants who took part in this activity were 83 students using the pretest and posttest methods. Results: The results obtained were an increase in the pretest score (before being given education) which was 62.11% to 83.22% in the posttest result (after being given education). Conclusion: There is a significant relationship between posttest scores, which increases after education is held, so pharmacoeconomics education needs to be given and pharmaceutical products utilization in health service followed by cost utillity evaluation and cost effectiveness as well as impact evaluation of quality adjusted life years of patients.