The chaperonin GroEL is a large complex composed of 14 identical 57-kDa subunits that requires ATP and GroES for some of its activities. We find that a monomeric polypeptide corresponding to residues 191 to 345 has the activity of the tetradecamer both in facilitating the refolding of rhodanese and cyclophilin A in the absence of ATP and in catalyzing the unfolding of native barnase. Its crystal structure, solved at 2.5 Å resolution, shows a well-ordered domain with the same fold as in intact GroEL. We have thus isolated the active site of the complex allosteric molecular chaperone, which functions as a "minichaperone." This has mechanistic implications: the presence of a central cavity in the GroEL complex is not essential for those representative activities in vitro, and neither are the allosteric properties. The function of the allosteric behavior on the binding of GroES and ATP must be to regulate the affinity of the protein for its various substrates in vivo, where the cavity may also be required for special functions.

In many reliability design and model-based health management applications where load profiles are variable and unpredictable, it is desirable to have efficient cycle counting methods to identify equivalent full and half cycles within the irregular load profile. Conventional cycle-based lifetime models can then be applied directly to provide information about the life consumption of the products. The use of an off-line rainflow algorithm is a common solution for arbitrary loads, but it cannot be applied in real time in its original form. This paper presents an in-line coding algorithm which uses a stack-based implementation, and a recursive algorithm to pick out the equivalent full and half cycles of the irregular load profile. The method can be integrated easily within time-domain or serial data applications to generate equivalent full and half cycles as they occur. Thus it is of particular significance for life estimation in real-time applications where use of the traditional implementations of the counting algorithm is impractical. In comparison with the off-line traditional rainflow method, the on-line method doesn't require any knowledge of the time history of the load profile because it processes each minimum or maximum when it occurs. Therefore, it provides a more efficient cycle counting method using less memory storage, and making more efficient use of computational resources within the real-time environment.

No abstract

No abstract

Abstract-The influence of selected control strategies on the level of low-order current harmonic distortion generated by an inverter connected to a distorted grid is investigated through a combination of theoretical and experimental studies. A detailed theoretical analysis, based on the concept of harmonic impedance, establishes the suitability of inductor current feedback versus output current feedback with respect to inverter power quality. Experimental results, obtained from a purpose-built 500-W, three-level, half-bridge inverter with an --output filter, verify the efficacy of inductor current as the feedback variable, yielding an output current total harmonic distortion (THD) some 29% lower than that achieved using output current feedback. A feed-forward grid voltage disturbance rejection scheme is proposed as a means to further reduce the level of low-order current harmonic distortion. Results obtained from an inverter with inductor current feedback and optimized feed-forward disturbance rejection show a THD of just 3% at full-load, representing an improvement of some 53% on the same inverter with output current feedback and no feed-forward compensation. Significant improvements in THD were also achieved across the entire load range. It is concluded that the use of inductor current feedback and feed-forward voltage disturbance rejection represent cost-effect mechanisms for achieving improved output current quality.