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Keeping the student focused for long periods of time has been a challenge for teachers. The adoption of new teaching strategies in which students leave the position of listeners and share with the master the active position is already known and even adopted. These strategies give students the opportunity to have more interactive experiences, stimulate independent thinking, create discussions within the group thus contribute to the improvement of the teaching- learning process. In that sense, the use of educational games has been an alternative, as they can mitigate the difficulty that students have in making the parallel between the concepts theorists learned from situations of application of this knowledge. Monitor the growth of the literature and research patterns in a given thematic area is an important step to understand the development of this domain. Therefore, the bibliometrics has been an emerging area, as it aims to describe, evaluate and monitor research published in a given field of knowledge. In this way, it is defined as the general objective of this work to map and characterize publications on the use of games and related terms in Production Engineering from bibliometric research in the annals of the National Meeting of Production Engineering. In this research, techniques such as Citation, Bibliographic Coupling, Co-author, Co-citation and Co- word were used, from which bibliometric maps are generated with the aid of the VOSviewer software, in which, for example, the most used keywords are shown; institutions and authors who publish works in the field; the authors who are engaged bibliographically; those who work together; and those that appear simultaneously in the reference lists.

This study aims to evaluate the cellular effects of amino benzazolo [3,2- a] quinolinium drugs on normal lymphoblast cells and compare them to effects on A431 epitheloid carcinoma cells. The drugs under study are amino containing heterocyclic compounds possessing a positive charge that could facilitate their interaction with cell organelles specially mitochondria. The fluorescent properties could also facilitate observation of their cellular binding location. These compounds have also been demonstrated to have mutagenic and DNA binding capacity on A431 cancer cells. A431 and TK6 cells in culture where exposed for 48 hours to determine the IC50 dose, mitochondrial membrane permeability, ROS generation and fluorescent microscopy analysis of intracellular binding. IC50 was determined by trypan blue exclusion and mitochondrial membrane permeability applying the Nucleo counter 3000, JC-1 assay. For ROS determination the fluorescent dye 2,7-dichlorofluorescein diacetate (DCFH-DA) was applied. Determination of intracellular binding was performed applying confocal microscopy. In the TK6 normal lymphoblast cells the observed IC50 concentrations were as follow: ABQ38=310µM, and ABQ95=270µM. On A431 IC50s were as follows: ABQ38 = 32 µM and ABQ95 = 36 µM. Overall, normal TK6 demonstrated higher tolerance to the tested ABQS than A 431. Preliminary results on ROS generation indicated a stronger ROS release on A431 than on TK6 lymphoblast. Analysis of the effects of ABQS on the mitochondrial permeability where also consistent with the cytotoxicity observed, demonstrating higher levels of membrane permeability on treated tumor cells than on normal. Microscopy images of ABQS treated cells revealed location of these fluorescent compounds in organelles such as mitochondria and DNA. DNA fragmentation analyses are being implemented. This study presents preliminary evidence of an overall higher tolerance of normal TK6 lymphoblast to the tested fluorescent drugs in comparison to treated A431. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4474. doi:10.1158/1538-7445.AM2011-4474

O presente trabalho mostra o uso de ferramentas da qualidade para selecionar, estabelecer, ordenar e propor soluções para os problemas que interferem na satisfação dos clientes durante o atendimento de uma empresa do setor bancário da cidade de Governador Valadares-MG.

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