Artemisia absinthium L. is a medicinal plant largely used in traditional medicine. The aim of this study was to estimate the content of polyphenols, and flavonoids compounds and also to evaluate the antioxidant and the anti-ulcer activities of the Aqueous extract from Artemisia absinthium L. aerial parts. The Folin-Ciocalteau and AlCl3 methods were applied in order to quantify the polyphenolic and flavonoids contents, respectively. However, DPPH   method was used to evaluate the in vitro antioxidant activity. Quantitative analysis of the yield and phenolic content of the aqueous extract of Artemisia absinthium showed that the yield of the aqueous extract was 19.32% and its phenolic content was 58.66 ± 2.16 μg GAE / mg dry extract for polyphenols and 6.85 μg QE / mg dry extract for the flavonoids. The antioxidant activity of the plant extract evaluated by the DPPH test is very important (IC50=45.48±0.37 µg/ml). Treatment of mice with the aqueous extract of Artemisia absinthium at a dose of 400 mg / kg significantly reduced the ulcerogenic effect of ethanol on the gastric wall with an estimated protection rate of 91%. These findings suggest that Artemisia absinthium L.  aqueous extract possessed good antiulcer and antioxidant potentials. This supports the traditional claims of this plant in folklore medicine. Keywords: Artemisia absinthium, polyphenols, antioxidant activity, gastric ulcer, ethanol.

Reactive oxygen (ROS) and nitrogen species (RNS) are produced in all cells and play important roles in physiology. The loss of the redox balance, either by an increase of oxidant molecules ROS and RNS or by decreased antioxidant system activities cause a state of oxidative stress. Several studies are going on worldwide directed towards finding natural antioxidants of plant origin. Plants containing phenolic compounds have been reported to possess strong antioxidant activity. The objective of this study is to evaluate total polyphenols and flavonoids contents (TPC and TFC) as well as examine the in vitro antioxidative properties from aqueous extract of Ammoides atlantica (AqE). TPC was estimated utilizing Folin-Ciocalteu's reagent. TFC was evaluated utilizing the aluminum chloride method. The antioxidant properties were evaluated using metal chelating and cupric ion reducing antioxidant capacity (CUPRAC) assays. Indeed, results showed that the AqE is rich in polyphenols (141.74±0.44 µg gallic acid equivalents/ mg of dry weight), and flavonoids (61.87±6.7 µg quercetin equivalent/ mg dry weight). These phytochemical compounds possess significant antioxidant activities. The results showed that AqE exhibited a good Metal chelating activity with an IC50 of 36.57±4.73 µg/ mL. CUPRAC assay showed that AqE extract exhibited high cupric ion reducing antioxidant capacity with an A0.5 of 8.58±0.13 µg/mL. These findings provide evidence that AqE of Ammoides atlantica is a potential source of antioxidant which have many benefits towards human health. Keywords: Ammoides atlantica, aqueous extract, phenolic compounds, metal chelating and cupric ion reducing antioxidant capacity.

This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants. Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.

Background: Matricaria chamomilla L. (M. chamomilla) is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. Our aims in this study was thus to quantify the phenolic, flavonoids contents in the flower of this plant, and also to evaluate the in vitro antioxidant potential and the in vivo analgesic activity. Methods: The total phenolic and flavonoid contents of the plant aqueous extract (MCAqE) were estimated using the Folin–Ciocalteu and AlCl3 colorimetric methods, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Analgesic activity was tested by acetic acid induced writhing model in mice. Results: Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 158.41±1.6 mg gallic acid equivalent/g of dry extract and 37.06±0.56 mg quercetin equivalent/g of dry extract, respectively. The antioxidant activity of the plant extract was important (IC50=3.08±0.25 mg/mL). MCAqE extract, at 400 mg/kg, showed analgesic activity (39.60±8.70%) against acetic acid induced pain in mice while the standard reference drug Diclofenac sodium exhibited 90.44±2.80% activity at 10 mg/kg dose.

Phlomis crinita is a plant species of the family Lamiaceae including more than 100 perennial herbs, shrubs, and sub-shrubs species native to the Mediterranean, Central Asia, and India. This species is commonly a good natural source of various secondary metabolites. Therefore, the present study was conducted to determine phenolic content and antioxidant activity of hydromethanolic (PC ME) and aqueous extracts (PC AQE) of aerial parts of P. crinita. Total polyphenols, flavonoids and tannins were quantified, respectively by the methods of Folin-Ciocalteu reagent, aluminum trichloride (AlCl3) and Bate-Smith method. The in vitro antioxidant activity was assessed using DPPH and ABTS•+ radical scavenging, β-carotene-linoleic acid, reducing power and ferrous ion chelating activity assays. PC ME showed high level of tannins (132,13 ±0.68 µg TAE/mg extract) and total phenolic content (82.71±0.79 µg GAE/mg extract), in addition a marked inhibiting oxidation activity of β-carotene/ linoleic acid (74.10%) was observed. Results showed also a higher iron-chelating activity of PC ME (0.20 mg/mL) compared to PC AQE (0.046 mg/mL). The plant extracts revealed a significant antioxidant activity as evidenced by the DPPH and ABTS radical scavenging activity (IC50 = 0.103 mg/mL for PC ME and 0.144 mg/mL for PC AQE) for DPPH assay and (IC50 = 0.0130 mg/mL for PC ME and 0.0187 mg/mL for PC AQE) , as well as the PC ME exhibits higher reducing power (IC50 =0.288mg/mL) than PC AQE (0.296 mg/mL). As a result, P.crinita is suggested as a promising and effective therapeutic medicinal plant for the treatment of several diseases.