Metalloproteinase-2 (MMP-2) and -14 (MMP-14) and the tissue inhibitor of metalloproteinases type 2 (TIMP-2) participate in epithelial-mesenchymal transition and tumor progression in many cancers. However, the correlation between these enzymes in gastric cancer and the metastatic potential to their respective lymph node needs to be determined. Here, we evaluated the expression of these enzymes in gastric carcinoma and lymph node metastases and their possible involvement in tumor progression. Histological samples from 83 patients with gastric cancer and their respective lymph nodes were used. MMP-2, MMP-14 and TIMP-2 immunoexpression was scored. TIMP-2 expression in tumor-associated macrophages occurred more frequently than in normal mucosa (P = 0.0128). Female tumor samples presented higher MMP-2 expression (P = 0.0248), while TIMP-2 occurred mainly in patients over 50 years old (P = 0.0034). MMP-2 was higher expressed in primary tumor macrophages than in neoplastic cells (P = 0.0118), and was also seen in macrophages from metastatic-affected lymph nodes of intestinal and diffuse histotypes (P = 0.0006). MMP-2, MMP-14 and TIMP-2 expression in mononuclear cells might be correlated with progression of gastric cancer. MMP-14 production by macrophages appears to be more involved in diffuse gastric cancer progression.

Este artigo está publicado em acesso aberto (Open Access) sob a licença Creative Commons, que permite uso, distribuição e reprodução em qualquer meio, sem restrições, desde que o trabalho seja corretamente citado. PERFIL NUTRICIONAL DE CRIANÇAS PORTADORAS DO TRANSTORNO DO ESPECTRO AUTISTA Nutritional profile of children bearing autism spectrum disorderPerfil nutricional de niños portadores de trastorno del espectro autista

PTEN is the most commonly inactivated tumor suppressor gene in primary prostate cancer (PCa) and its loss is associated with poor clinical outcomes. ERG rearrangement is a genomic alteration frequently found in PCa and its prognostic significance has yielded mixed results. Although the association of PTEN and ERG biomarkers has potential impact on clinical outcomes, studies examining the two genes simultaneously are scarce in Brazilian populations. In this study, we retrospectively examined the relationship between ERG expression and PTEN loss in 119 surgically treated prostate cancer patients from Northeastern Brazil through immunohistochemical analysis. ERG expression was found in 41.0% (48/117) of cases and the loss of PTEN detected in 38.1% (40/105) of samples. ERG-positive cases were significantly associated with lower prostate weight; ERG negatively correlated with Gleason score above 6. The lack of associations for PTEN loss alone in this cohort is counter to the literature, which shows that PTEN loss is usually associated with more aggressive disease. The overlapping of the two biomarkers revealed that samples with positive ERG expression without PTEN loss were associated with lower Gleason and lower Grade group. This study contributes with the discussion about the development of the molecular profiling of prostate cancer. The further development of similar studies could help in stratifying specific risk groups, leading to a more personalized therapeutic decision for prostate cancer treatment.

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Introdução: A sarcopenia atualmente é considerada como uma doença crônica não transmissível, consistindo na perda ou diminuição da musculatura esquelética, da potência e consequentemente da força. Objetivo: buscar correlações entre o consumo alimentar com a prevalência de sarcopenia em idosos de duas cidades do Ceará, observando se os hábitos alimentares deles influenciam no diagnóstico de sarcopenia. Métodos: estudo de caráter quantitativo, transversal descritivo e analítico realizado com 65 idosos residentes em duas cidades, Fortaleza e Icó. Foi aplicado questionário sociodemográfico, recordatório alimentar de 24 horas e o teste do Algoritmo. A análise estatística foi realizada por meio dos testes Qui-quadrado e Mann-Whitney. Resultados: A patologia esteve presente nas duas cidades, apresentando uma maior prevalência nos homens da cidade de Icó (23,1%) e nas mulheres da cidade de Fortaleza (42,8%), comparando o mesmo gênero entre os municípios. A ingestão de carboidratos e lipídios apresentou-se deficiente em ambas as cidades, enquanto a ingestão proteica ultrapassou a recomendação da RDA. Relacionando os micronutrientes, apenas a vitamina C apresentou diferença significativa (p<0,05) entre os grupos, sendo sua ingestão adequada nos idosos não sarcopênicos. Conclusão: Hábitos alimentares com maior ingestão de vitamina C foram associados à prevenção da sarcopenia.