This research was conducted to determine the antibacterial activity of BSF extract in vitro on the growth of Salmonella typhimurium, E. coli and Pseudomonas aureginosa. The experiment was carried out according to the Completely Randomized Design (CRD) consisting of six treatments and three replications for each treatment. The treatments were different concentration levels of BSF extract, i.e. 75, 125, 175, 225, 275 and 325 mg.ml−1. Chloramphenicol with concentration of 30 µg. discs paper−1 was used as a positive control and dimethyl sulfoxide (DMSO) as a negative control. BSF extract was made using maceration extraction method. The results of this study indicated that the antibacterial activity of BSF extract increased (P<0.05) in line with the increase level of BSF extract concentration. The average diameter of the inhibition zone for Salmonella typhimurium, E. coli and Pseudomonas aureginosa was 11.77 ± 0.03 mm, 11.15 ± 0.05 mm, and 11.15 ± 0.23 mm respectively, which was categorized as strong inhibition zone. In conclusion, the concentration of BSF extract of 325 mg.ml−1 is an effective concentration to inhibit the growth of the bacteria Salmonella typhimurium, E. coli and Pseudomonas aureginosa.

T here is a continuous increase in the growth of the animal husbandry industry in Indonesia, especially the poultry sector, due to the increasing demand for related products and population growth. The local village chicken plays an essential role in the lives of Indonesian and is associated with cultural development, especially in rural areas, where it is a major source of meat and eggs to fulfill the nutritional demands of the people. However, the development of local poultry in the country, especially village chicken, is still very slow compared to commercial chicken breeds. This condition is due to the relatively low genetic potential, inadequate management, and the use of traditional feeds with low quality (Nataamijaya, 2017). According to Budiansyah ( 2010), approximately 70-80% full-scale fabrication value of livestock business depends on the feed. Therefore, the optimal quality and quantity of feed are needed to improve growth.One of the effects of Covid-19 is the disruption in the supply of mostly imported protein source feedstuffs, such as fish and soybean meal. These ingredients are required to formulate a ration containing balanced nutrition to meet the needs of poultry for optimal production. Consequently, the price of these feed ingredients is increasing. According

ABSTRAKPenelitian bertujuan untuk mengkaji pengaruh penambahan ramuan herbal yang dikombinasikan dengan ekstrak kerang bakau (Polymesoda erosa) di dalam pakan terhadap kadar kolesterol, asam urat dan glukosa darah ayam buras. Penelitian menggunakan 100 ekor ayam buras yang dipelihara mulai dari umur satu hari (DOC) sampai dengan umur 16 minggu. Penelitian menggunakan rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan. Perlakuan terdiri atas R0 (pakan basal + 0% probiotik herbal + 0% ekstrak kerang bakau), R1 (pakan basal + 0,75% probiotik herbal + 1,5% ekstrak kerang bakau), R2 (pakan basal + 1,5% probiotik herbal + 3% ekstrak kerang bakau), R3 (pakan basal + 2,25% probiotik herbal + 4,5% ekstrak kerang bakau) dan R4 (pakan basal + 3% probiotik herbal + 6% ekstrak kerang bakau). Data yang diperoleh dari hasil penelitian dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa rataan kolesterol darah yang diperoleh adalah berturut-turut 185,00±7,30 (P0), 185,75±6,80 (P1), 153,25±19,06 (P2), 167,75±17,72 (P3), 214,00±19,18 (P4). Rataan asam urat dalam darah yang diperoleh adalah 3,95±0,58 (P0), 6,70±4,23 (P1), 4,10±0,64 (P2), 4,32±1,27 (P3), 6,30±2,27 (P4). Sedangkan rataan glukosa darah yang diperoleh adalah 231±20,19 (P0), 188,50±24,63 (P1), 223,50±32,19 (P2), 227,25±68,61 (P3), 236,50±28,43 (P4).   Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian ramuan herbal dan ekstrak kerang bakau dalam pakan dapat mempengaruhi kadar kolestrol darah, namun tidak mempengaruhi kadar asam urat dan glukosa darah ayam buras. Kadar kolesterol darah terendah diperoleh pada perlakuan R2 dan R3 yang nyata berbeda dengan perlakuan R0, R1 dan R4.Kata Kunci: ayam buras, ramuan herbal, kolesterol, asam urat, glukosa darah.ABSTRACTKeywords:  blood glucose, cholesterol, herbal concoction, uric acid, village chickenThe objectives of this research was to evaluate the effect of agricultural by product-based feed supplemented with herbal concoction (HC) and mud clams extract (MC) on blood cholesterol, uric acid and blood glucose concentration. Completely randomized design was used with 4 treatments and 4 replications.  Ninety day old chick of local village chicken was used in this experiment and kept up to 16 weeks during the experiment. The treatments consisted of basal feed (R0), basal feed + 0.75% HC + 1.5% MC (R1), basal feed + 1.5% HC + 3.0% MC (R2), basal feed + 2.25% HC + 4.5% MC (R3), basal feed + 3.0% HC + 6.0% MC (R4).  All data were analyzed using analysis of variance and different between treatments were proved using Duncan multiple range test. The results showed that the concentrations of blood cholesterol gained (mg/dl) were 185.00±7.30 (P0), 185.75±6.80 (P1), 153.25±19.06 (P2), 167.75±17.72 (P3), 214.00±19.18 (P4). The concentration of uric acid gained (mg/dl) were 3.95±0.58 (P0), 6.70±4.23 (P1), 4.10±0.64 (P2), 4.32±1.27 (P3), 6.30±2.27 (P4). Whereas, the glucose concentration gained (mg/dl) were 231.00±20.19 (P0), 188.50±24.63 (P1), 223.50±32.19 (P2), 227.25±68.61 (P3), 236.50±28.43 (P4). Based on variance analysis it was concluded that the treatments gave a significant effect (P<0.05) on blood cholesterol concentration, but had no effect (P>0.05) on uric acid and blood glucose concentration. Blood cholesterol concentrations were significant lower in treatment R2 and R3 compared to treatment R0, R1 and R4.Keywords:  blood glucose, cholesterol, herbal concoction, uric acid, village chicken

Penelitian ini bertujuan untuk melihat level dan waktu inkubasi terbaik pemberian EM4 terhadap nilai nutrisi kulit ari kedelai. Penelitian akan dilaksanakan di Laboratorium Nutrisi dan Makanan Ternak Jurusan Peternakan Fakultas Peternakan Universitas Halu Oleo, Kendari. Penelitian ini menggunakan metode percobaan laboratorium dengan Rancangan Acak Lengkap (RAL) dengan pola faktorial. Faktor pertama adalah pemberian level EM-4 , yaitu 0 cc, 1 cc, 2 cc dan 3 cc, faktor kedua ialah waktu inkubasi yang berbeda, yaitu inkubasi 0 jam, inkubasi 24 jam, inkubasi 48 jam, dan inkubasi 72 jam yang masing-masing perlakuan diulang 3 kali. Peubah yang diamati adalah bahan kering, abu, protein kasar, dan serat kasar. Hasil penelitian ini menunjukkan bahwa pemberian level EM-4 dan waktu inkubasi yang berbeda pada tepung kulit ari biji kedelai memberikan perbedaan nyata (P<0.05) pada nilai kadar abu dan serat kasar, tetapi tidak berbeda nyata (P>0.05) pada nilai bahan kering dan protein kasar, namun ada peningkatan atau perbaikan protein kasar dari tepung kulit ari biji kedelai pada level pemberian 3 cc EM-4 dan waktu inkubasi 72 jam.

Ninety head day old chick (DOC) of local village chicken was used in this experiment to evaluate the effect of agricultural by product-based feed supplemented with herbal probiotics (HP) and mud clams extract (MC) on production performance of local village chicken. A completely randomized design was used with 5 treatments and 4 replications. The treatments consisted of control (R0), 0.75%HP+1.5%MC (R1), 1.5%HP+3%MC (R2), 2.25%HP+4.5%MC (R3), and 3.0%HP+6.0%MC (R4). All chicken was kept in a group of five flocks equipped with feeding and water supply apparatus. The results showed that local village chicken got R2 treatment gave a significantly higher response in feed consumption (32.5gd-1) compared to R0 (23.1gd-1), R1 (23.9gd-1), R3 (24.0gd-1), and R4 (21.3gd-1). The daily gain of local village chicken was also significantly higher in R2 treatment (7.4g-1) compared to R0 (4.6gd-1), R1 (4.9gd-1), R3 (4.6gd-1), and R4 treatment (4.1gd-1). However, feed conversion resulted from those treatments did not show a significant difference. The lower feed conversion gain in this experiment has existed in R2 treatment (4.0) followed by treatment R1 (4.9), R0 (5.0), R4 (5.2), and treatment R3 (5.4). Therefore, it was concluded that the treatments had a significant effect on feed consumption and daily gain but had no effect on feed conversion of local village chicken. The inclusion level of 1.5% herbal probiotics and 3% mud clams extract gave better effect on both feed consumption and daily gain of local village chicken.