No abstract

No abstract

Total DNA was extracted from the leaves of Atractylodes lancea DE CANDOLLE, A. ovata DE CANDOLLE and A. japonica KOIDZUMI ex KITAMURA of various origins and hybridized with digoxigenin-labeled rice ribosomal DNA after digestion with eight different restriction endonucleases. The resulting restriction fragment length polymorphism (RFLP) profiles allowed us to distinguish the three Atractylodes species when DNA was digested with Sac I. Although atractylon was detected in the rhizomes of some of the cultivated strains of A. lancea, their RFLP profiles clearly indicate that these plants are not hybrids of A. ovata or A. japonica. RFLP analysis also revealed the presence of intraspecific variation in DNA sequence of rRNA locus among A. lancea as well as A. japonica.

No abstract

Shoot formation was indticcd from the tip tissue of Rehmannia cil f. bueichingen.tis on hormone-free Murashige-Skoog medium within 4 weeks of culture. Shoots were propagated on Murashige-Skoog medium supplemented with l.Umg I ben zy lade nine for 4 weeks. PropagiUed shoots rooted on the hormone-free Murashige-Skoog medium during 4 weeks of culture. Total DNA was extracted from the leaves of a F, hybrid and its parents. R. glulinosa f. huekliingensis and R. glutinosa var, purpitrcit. Analysis of random-amplified polymorphic DNA (RAPD) using II) arbitrary oligonucleotide lO-mers. showed the genetic homogeneity ofthe above three species. The F, hybrid was genetically intermediate between both parental plants, compared with the genetic distance between the F, hybrid and individual parents. Furthermore, the comparison of the band patterns between the F, hybrid, obtained from ihe crossing cleariy showed thai parts ofthe bands of both parents. R. glutino.ta f. hueUlitngensis and R. ijhuinosa var. piirpurea, were introduced into the F, hybrid.