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There is a growing concern about the rapid rise in resistance of Staphylococcus aureus to antimicrobial agents. Our objective was to determine the prevalence and pattern of antibiotic sensitivity among Methicillinresistant and Methicillin-sensitive Staphylococcus aureus in Surat, South Gujarat, India. Covering the period of three months from August-2004 to October 2004, we processed the samples of Pus, Urine, Blood, high vaginal swabs, Sputum, throat swabs, drains and ear swabs received from New Civil Hospital, Surat. Total 135 Staphylococci were isolated, out of which, 48 (35.55%) were coagulase positive. These coagulase positive Staphylococci isolates were screened for Methicillin-resistance by a slide latex agglutination kit for the rapid detection of PBP2' (Penicillin binding protein 2a). Sensitivity to amikacin, erythromycin, clindamycin and tetracycline were also carried out following Kirbey Bauer disc diffusion method. Methicillin resistance among the Staphylococcus aureus isolates was 39.5%. Resistance to all antibiotics tested among the Methicillinresistance and Methicillin-sensitive, staphylococci was found to be 26.3% and 6.8% respectively, which is statistically signiÞ cant. Methicillin-resistance is a useful marker in selecting appropriate antimicrobial agents for treatment of infections caused by S. aureus changing pattern of resistance of S. aureus makes its periodic surveillance mandatory.

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Background: Healthcare-associated infections (HAIs) are a major global threat to patient safety. Systematic surveillance is crucial for understanding HAI rates and antimicrobial resistance trends and to guide infection prevention and control (IPC) activities based on local epidemiology. In India, no standardized national HAI surveillance system was in place before 2017. Methods: Public and private hospitals from across 21 states in India were recruited to participate in an HAI surveillance network. Baseline assessments followed by trainings ensured that basic microbiology and IPC implementation capacity existed at all sites. Standardized surveillance protocols for central-line–associated bloodstream infections (CLABSIs) and catheter-associated urinary tract infections (CAUTIs) were modified from the NHSN for the Indian context. IPC nurses were trained to implement surveillance protocols. Data were reported through a locally developed web portal. Standardized external data quality checks were performed to assure data quality. Results: Between May 2017 and April 2019, 109 ICUs from 37 hospitals (29 public and 8 private) enrolled in the network, of which 33 were teaching hospitals with >500 beds. The network recorded 679,109 patient days, 212,081 central-line days, and 387,092 urinary catheter days. Overall, 4,301 bloodstream infection (BSI) events and 1,402 urinary tract infection (UTI) events were reported. The network CLABSI rate was 9.4 per 1,000 central-line days and the CAUTI rate was 3.4 per 1,000 catheter days. The central-line utilization ratio was 0.31 and the urinary catheter utilization ratio was 0.57. Moreover, 3,542 (73%) of 4,742 pathogens reported from BSIs and 868 (53%) of 1,644 pathogens reported from UTIs were gram negative. Also, 1,680 (26.3%) of all 6,386 pathogens reported were Enterobacteriaceae. Of 1,486 Enterobacteriaceae with complete antibiotic susceptibility testing data reported, 832 (57%) were carbapenem resistant. Of 951 Enterobacteriaceae subjected to colistin broth microdilution testing, 62 (7%) were colistin resistant. The surveillance platform identified 2 separate hospital-level HAI outbreaks; one caused by colistin-resistant K. pneumoniae and another due to Burkholderia cepacia. Phased expansion of surveillance to additional hospitals continues. Conclusions: HAI surveillance was successfully implemented across a national network of diverse hospitals using modified NHSN protocols. Surveillance data are being used to understand HAI burden and trends at the facility and national levels, to inform public policy, and to direct efforts to implement effective hospital IPC activities. This network approach to HAI surveillance may provide lessons to other countries or contexts with limited surveillance capacity.Funding: NoneDisclosures: None

Candida speciesis a part of commensalin healthy humans but they can cause opportunistic infections, especially in immunocompromised individuals, patient admitted in ICUs(Intensive care units) and HIV patients. biofilm act as protective shield of microorganism. Candida species form most common fungal biofilm, which is extremely difficult to treat. Purpose: The biofilm forming candida is difficult to eradicate with usual antifungal drug and often cause chronic infections. Understanding of biofilm process is very important for effective control strategies of biofilm associated infections and improvement in patient management. Method:The observational cross sectional prospective study was conducted on forty patients samples including urine, BAL/sputum, blood culture, body fluids, pus, swab, indwelling devicesand tissue showing candida species growth. Biofilm was detected using pre-sterilized 96 well polystyrene microtitre plate method. Results: Among 40 isolates, 22 isolates were detected positive, whereas 18 isolates were negative for biofilm formation. C. tropicalis had formed maximum strong biofilms among all species isolated. These biofilm acts resisting antifungal treatment and withstanding the competitive pressure from other organisms, these are difficult to treat.Changing trend with shift toward non albicans. Conclusion:Candida tropicalis as the predominant pathogen causing candidia infectionsand it had highest capacity to form biofilm.