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The effects of several concentrations of NO2, NaNO3, and NaNO2 respectively, upon strain L, mouse liver cells, and HeLa cells, were studied and a modified system designed to permit continuous exposure of cells to air pollutants is described. In NCTC medium 109 containing serum, cells tolerate concentrations of NO2 up to 4100 p.p.m. and some may even tolerate 8600 p.p.m. Removal of the serum lowers the lethal concentration of NO2 to less than 100 p.p.m. If the cells were covered only by a thin film of BSS (balanced salt solution) medium, a concentration of 100 p.p.m. NO2 proved toxic within [Formula: see text] hour. If, however, the NO2 concentration was reduced to 5 or 10 p.p.m., cells survived a daily 8-hour exposure but many, if not most, of the cells were dead after several days. The presence of as little as 25 mg% NaNO2 retarded proliferation. On the other hand, NaNO3 was tolerated well in the three cell lines tested; HeLa cells seemed to be the most sensitive of the cell strains with respect to these salts.

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